The PR1001-R-1000 is the Red Dye variant of the Accuris Taq 2X Master Mix at the 1,000-reaction pack format — same Taq + buffer + dNTPs + MgCl₂ formulation as the base PR1001-1000, with inert red gel loading dye pre-formulated in the master mix for direct gel loading after PCR (no separate loading-dye supplementation step). The PCR product is ready to load directly onto the gel as soon as cycling completes — a meaningful workflow ergonomic for production PCR-to-gel benches and high-throughput screening labs. Ships as 20 × 1.25 mL tubes at the same $393 list price as the dye-free PR1001-1000 — Red Dye is bundled at no price premium.
Key Specifications
| SKU | PR1001-R-1000 |
| Type | Taq 2X Red Dye Master Mix — pre-formulated with red gel-loading dye |
| Reactions per pack | 1,000 reactions (pack format: 20 × 1.25 mL tubes) |
| Concentration | 2× master mix |
| Polymerase | Taq DNA Polymerase (standard, non-hot-start) |
| Buffer composition | PCR enhancers, 2 mM dNTPs, 6 mM MgCl₂, inert red gel loading dye |
| Direct gel loading | Yes — load PCR product directly onto gel after cycling, no separate loading-dye step |
| Smaller pack sister | PR1001-R-200 (200 reactions, 4 × 1.25 mL) |
| Dye-free sister | PR1001-1000 (same chemistry without Red Dye, same price) |
| Hot Start + Red Dye | PR1001-HSR-1000 (Hot Start + Red Dye combined master mix) |
| List price | $393 |
| Returns | Non-returnable — perishable reagent per Accuris reagent-return policy |
Why Red Dye in the Master Mix
The pre-formulated red gel loading dye delivers two workflow ergonomic benefits:
- Direct gel loading after PCR — when cycling completes, the PCR product is already prepared for gel loading. No separate loading-dye supplementation step (no dispensing 6× loading dye into each PCR tube before loading on the gel). The operator pipettes directly from the PCR tube to the gel well.
- Visual reaction tracking during setup — the red color is visible during master-mix dispensing and reaction setup, providing a visual indicator that helps detect missed wells or pipetting errors during high-throughput reaction setup.
The dye is inert — it doesn't interfere with PCR cycling, polymerase activity, or downstream analysis. The molecular weight of the dye is positioned for standard PCR product visualization: the dye migrates in the gel at a position that is visually trackable during the run without overlapping with PCR products in typical sizing ranges.
Red Dye vs Dye-Free — When to Pick Each
| Variant | Direct gel load? | Use case |
| PR1001-R-1000 (this product) | Yes | Production PCR-to-gel benches, high-throughput screening, teaching labs running gel-electrophoresis exercises after PCR |
| PR1001-1000 (no dye) | No (separate loading-dye supplementation step required) | Workflows where PCR product is not loaded on a gel (e.g., PCR product feeds directly into a downstream cloning, sequencing, or qPCR-quantitation step where the dye would interfere) |
Pick the Red Dye variant when the PCR-to-gel workflow is the standard endpoint — most routine PCR analytical workflows fall into this category. Pick the dye-free PR1001-1000 when downstream processing (cloning ligation, sequencing prep, fluorescent-detection qPCR) requires a clean PCR product without loading-dye interference.
Same price ($393) on both variants — choice is purely workflow-ergonomic, not cost-driven.
Pack-Size Family — PR1001-R Red Dye Master Mix
| SKU | Reactions | Pack format | Use case |
| PR1001-R-200 | 200 | 4 × 1.25 mL | Smaller pack — pilot or occasional gel-loading workflows |
| PR1001-R-1000 (this product) | 1,000 | 20 × 1.25 mL | Mid-throughput / production PCR-to-gel workflow |
Reagent Composition
Per the Accuris Life Science Reagents Catalog, each PR1001-R-1000 pack includes:
- Taq DNA Polymerase (pre-formulated in 2× master mix)
- Buffer — PCR enhancers, 2 mM dNTPs, 6 mM MgCl₂
- Inert red gel loading dye — formulated at the standard loading-dye concentration
Standard reaction setup: combine 25 µL of master mix + 25 µL of (primers + template + water) in a 50 µL reaction. After cycling, load PCR product directly onto the gel — the red dye is already at the standard loading-dye concentration in the 50 µL reaction.
Application Profile
Per the Accuris Life Science Reagents Catalog, Taq 2X Master Mix applications (which apply to the Red Dye variant as well):
- Colony PCR — direct PCR from bacterial colony for clone screening, gel-load directly to confirm clone insert size
- Fast PCR — short-amplicon PCR followed by direct gel analysis
- Genotyping — endpoint PCR followed by direct gel-load for SNP / indel call
- High-throughput PCR — production-scale screening with PCR-to-gel readout
- Routine PCR — any general-purpose endpoint PCR feeding into gel-electrophoresis analysis
The Red Dye master mix is specifically optimized for the PCR-to-gel workflow — the standard endpoint in most analytical PCR protocols.
When to Pick This Pack
Order PR1001-R-1000 when:
- Production PCR-to-gel workflow — labs running endpoint PCR with gel-electrophoresis as the readout at mid-to-high throughput
- Genotyping production benches — labs running cohort genotyping where every PCR product is gel-analyzed
- Teaching labs — molecular-biology teaching where direct-load saves operator time in cohort exercises
- High-throughput screening — production-scale screening workflows where the time saved on direct-load operations is meaningful across hundreds of reactions
- Multi-bench shared inventory — institutional procurement of standardized PCR master mix across multiple gel-loading benches
Ordering & Configuration
Storage & handling. Per Accuris kit instructions — typical PCR master-mix storage at −20 °C with limited freeze-thaw cycle tolerance. The 20-tube format supports rotating tubes through active use while keeping the rest in deep storage.
Downstream-workflow verification. Critical at order: confirm the PCR product is loaded on a gel (Red Dye variant is right) vs fed into cloning / sequencing / fluorescent qPCR (dye-free PR1001-1000 is right).
Hot-start variant consideration. For multiplex / GC-rich / low-copy workflows that also benefit from direct gel-loading, see PR1001-HSR-1000 (Hot Start + Red Dye combined).
Returns. Per the universe price-list category header: reagent products including PCR master mixes are non-returnable due to perishable nature.
Confirm at quote: workflow downstream-step (gel-load vs cloning / sequencing / qPCR), pack-size match (200 vs 1,000 reaction), Hot Start variant consideration, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
Accuris PR1001-R Red Dye Master Mix family:
- PR1001-R-200 — 200 reactions, 4 × 1.25 mL
- PR1001-R-1000 (this product) — 1,000 reactions, 20 × 1.25 mL
- PR1001-HSR-200 / PR1001-HSR-1000 — Hot Start + Red Dye combined master mix
- PR1001-1000 (dye-free) — same chemistry without Red Dye for non-gel downstream workflows
Pick the Red Dye variant for PCR-to-gel workflows; pick dye-free for non-gel downstream processing.
