The PR1000-HS-S is the trial / sample-size SKU of the Accuris Hot Start Taq DNA Polymerase family — 50 units of Hot Start Taq at 5 U/µL (10 µL total) for first-use evaluation of the Hot Start chemistry before committing to the larger production-scale packs (PR1000-HS-500 at $232 / PR1000-HS-1000 / PR1000-HS-6000 bulk). At $7.65 list price, the sample SKU enables labs to validate the Hot Start chemistry on their specific PCR target — particularly important for the multiplex / GC-rich / low-copy PCR workflows where Hot Start is the chemistry of choice.
Key Specifications
| SKU | PR1000-HS-S |
| Type | Sample / trial pack — Accuris Hot Start Taq DNA Polymerase |
| Quantity | 50 units (10 µL at 5 U/µL) |
| Polymerase concentration | 5 U/µL (standard Hot Start Taq concentration) |
| Hot-start mechanism | Antibody-mediated — polymerase inactive below ~95 °C, activated by initial denaturation step |
| Included buffer | 4 tubes of 5X buffer with PCR enhancers and 15 mM MgCl₂ |
| dNTPs | Not included — sold separately |
| Trial reaction count | ~25–50 reactions at 1–2 U/reaction |
| Sister SKUs (production scale) | PR1000-HS-500 (500 units, $232), PR1000-HS-1000 (1000 units), PR1000-HS-6000 (6000 units bulk) |
| PCR mode | Hot Start — antibody-mediated activation |
| List price | $7.65 |
Why a Hot Start Sample Pack — Workflow-Specific Validation
Hot Start chemistry is the right pick for specific PCR workflow scenarios rather than universal-replacement for standard Taq. The sample pack supports validation that the lab's workflow actually benefits from Hot Start before committing to bulk procurement:
| Workflow | Why Hot Start matters | Sample-pack validation step |
| Multiplex PCR | Multiple primer pairs in one reaction increase non-specific amplification risk; Hot Start prevents pre-activation extension | Run the multiplex panel with Hot Start vs standard Taq; confirm reduction in non-specific bands |
| GC-rich amplicons | GC-rich templates form secondary structures at low temperatures that Hot Start helps prevent | Run the GC-rich target; confirm improved on-target amplification |
| Low-copy PCR | Low-template PCR is sensitive to even small non-specific extensions; Hot Start preserves sensitivity | Run a serial dilution of template; confirm Hot Start sensitivity improvement vs standard Taq |
| Primer-dimer-prone setups | Primer pairs prone to dimerization benefit from Hot Start's pre-activation suppression | Compare primer-dimer band intensity Hot Start vs standard Taq |
Workflow benefit. The $7.65 sample evaluation cost supports the workflow-specific validation that justifies the Hot Start premium over standard Taq.
Hot Start vs Standard Taq vs HF Decision
| Feature | PR1000 Standard Taq | PR1000-HS Hot Start (this sample) | PR1000-HF High Fidelity |
| Polymerase concentration | 5 U/µL | 5 U/µL | 2 U/µL |
| Hot-start activation | None — active at all temps | Antibody-mediated — inactive below ~95 °C | None — proofreading instead |
| Proofreading | No | No | Yes |
| Best for | Routine endpoint PCR | Multiplex, GC-rich, low-copy, primer-dimer-prone | Sequencing-grade, cloning, mutagenesis |
Pick Hot Start over standard Taq when: the workflow is multiplex, GC-rich, low-copy, or primer-dimer-prone — Hot Start eliminates the pre-activation non-specific extension that hurts sensitivity / specificity in these workflows.
Pick HF over Hot Start when: sequence accuracy matters more than activation control (HF has lower error rate; Hot Start has cleaner activation but standard error rate).
Per Brochure Hot Start Applications
Per Accuris Life Science Reagents Catalog, Hot Start Taq applications include:
- Colony PCR — when colony-screen primer pairs are prone to non-specifics
- Fast PCR — Hot Start prevents pre-activation extension during the rapid setup
- GC-rich DNA amplification — Hot Start handles GC-rich secondary structures better
- Low Copy PCR — Hot Start preserves sensitivity by eliminating low-template non-specifics
- Multiplex PCR — the primary Hot Start application; multiple primer pairs benefit from controlled activation
Application Profile
Hot Start chemistry first-time evaluation — labs evaluating Accuris Hot Start Taq for the first time use the sample pack to validate fit before bulk procurement.
Multiplex assay validation — labs developing multiplex assays validate Hot Start performance on the multiplex panel before scaling to production.
GC-rich target validation — labs amplifying GC-rich targets validate Hot Start improvement vs standard Taq before committing.
Sales / training samples — sales reps and training programs use the sample pack for hands-on customer demonstrations.
Educational laboratory PCR exercises — molecular-biology coursework introducing hot-start chemistry concepts.
Method-development workflows — protocol-development workflows where Hot Start is one of several enzyme options being compared.
Ordering & Configuration
Single-use sample SKU. The 50-unit pack supports a multi-condition validation run; not intended for routine production use.
Pair with dNTPs. Hot Start buffer does not include dNTPs (unlike HF buffer) — confirm dNTP inventory at order. dNTPs sold separately.
Confirm at quote: Hot Start chemistry evaluation intent (vs production-pack ordering), workflow application fit (multiplex / GC-rich / low-copy), production-pack interest (PR1000-HS-500 follow-on), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1000-HS Hot Start Taq family:
- PR1000-HS-S (this product) — Sample, 50 units ($7.65) — first-use evaluation
- PR1000-HS-500 — 500 units ($232) — small lab production pack
- PR1000-HS-1000 — 1000 units — mid-throughput production pack
- PR1000-HS-6000 — 6000 units — high-throughput bulk pack
Pick the sample for Hot Start chemistry evaluation; step to the 500 / 1000 / 6000-unit packs for production workflows.
