The PR1000-HS-S is the trial / sample-size SKU of the Accuris Hot Start Taq DNA Polymerase family, 50 units of Hot Start Taq at 5 U/µL (10 µL total) for first-use evaluation of the Hot Start chemistry before committing to the larger production-scale packs (PR1000-HS-500 at $232 / PR1000-HS-1000 / PR1000-HS-6000 bulk). At $7.65 list price, the sample SKU enables labs to validate the Hot Start chemistry on their specific PCR target, particularly important for the multiplex / GC-rich / low-copy PCR workflows where Hot Start is the chemistry of choice.
Key Specifications
| SKU | PR1000-HS-S |
| Type | Sample / trial pack / Accuris Hot Start Taq DNA Polymerase |
| Quantity | 50 units (10 µL at 5 U/µL) |
| Polymerase concentration | 5 U/µL (standard Hot Start Taq concentration) |
| Hot-start mechanism | Antibody-mediated: polymerase inactive below ~95 °C, activated by initial denaturation step |
| Included buffer | 4 tubes of 5X buffer with PCR enhancers and 15 mM MgCl₂ |
| dNTPs | Not included (sold separately) |
| Trial reaction count | ~25–50 reactions at 1–2 U/reaction |
| Sister SKUs (production scale) | PR1000-HS-500 (500 units, $232), PR1000-HS-1000 (1000 units), PR1000-HS-6000 (6000 units bulk) |
| PCR mode | Hot Start (antibody-mediated activation) |
| List price | $7.65 |
Why a Hot Start Sample Pack: Workflow-Specific Validation
Hot Start chemistry is the right pick for specific PCR workflow scenarios rather than universal-replacement for standard Taq. The sample pack supports validation that the lab's workflow actually benefits from Hot Start before committing to bulk procurement:
| Workflow | Why Hot Start matters | Sample-pack validation step |
| Multiplex PCR | Multiple primer pairs in one reaction increase non-specific amplification risk; Hot Start prevents pre-activation extension | Run the multiplex panel with Hot Start vs standard Taq; confirm reduction in non-specific bands |
| GC-rich amplicons | GC-rich templates form secondary structures at low temperatures that Hot Start helps prevent | Run the GC-rich target; confirm improved on-target amplification |
| Low-copy PCR | Low-template PCR is sensitive to even small non-specific extensions; Hot Start preserves sensitivity | Run a serial dilution of template; confirm Hot Start sensitivity improvement vs standard Taq |
| Primer-dimer-prone setups | Primer pairs prone to dimerization benefit from Hot Start's pre-activation suppression | Compare primer-dimer band intensity Hot Start vs standard Taq |
Workflow benefit. The $7.65 sample evaluation cost supports the workflow-specific validation that justifies the Hot Start premium over standard Taq.
Hot Start vs Standard Taq vs HF Decision
| Feature | PR1000 Standard Taq | PR1000-HS Hot Start (this sample) | PR1000-HF High Fidelity |
| Polymerase concentration | 5 U/µL | 5 U/µL | 2 U/µL |
| Hot-start activation | None (active at all temps) | Antibody-mediated. Inactive below ~95 °C | None (proofreading instead) |
| Proofreading | No | No | Yes |
| Best for | Routine endpoint PCR | Multiplex, GC-rich, low-copy, primer-dimer-prone | Sequencing-grade, cloning, mutagenesis |
Pick Hot Start over standard Taq when: the workflow is multiplex, GC-rich, low-copy, or primer-dimer-prone. Hot Start eliminates the pre-activation non-specific extension that hurts sensitivity / specificity in these workflows.
Pick HF over Hot Start when: sequence accuracy matters more than activation control (HF has lower error rate; Hot Start has cleaner activation but standard error rate).
Per Brochure Hot Start Applications
Per Accuris Life Science Reagents Catalog, Hot Start Taq applications include:
- Colony PCR. When colony-screen primer pairs are prone to non-specifics
- Fast PCR. Hot Start prevents pre-activation extension during the rapid setup
- GC-rich DNA amplification. Hot Start handles GC-rich secondary structures better
- Low Copy PCR. Hot Start preserves sensitivity by eliminating low-template non-specifics
- Multiplex PCR. The primary Hot Start application; multiple primer pairs benefit from controlled activation
Application Profile
Hot Start chemistry first-time evaluation. Labs evaluating Accuris Hot Start Taq for the first time use the sample pack to validate fit before bulk procurement.
Multiplex assay validation. Labs developing multiplex assays validate Hot Start performance on the multiplex panel before scaling to production.
GC-rich target validation. Labs amplifying GC-rich targets validate Hot Start improvement vs standard Taq before committing.
Sales / training samples. Sales reps and training programs use the sample pack for hands-on customer demonstrations.
Educational laboratory PCR exercises. Molecular-biology coursework introducing hot-start chemistry concepts.
Method-development workflows. Protocol-development workflows where Hot Start is one of several enzyme options being compared.
Ordering & Configuration
Single-use sample SKU. The 50-unit pack supports a multi-condition validation run; not intended for routine production use.
Pair with dNTPs. Hot Start buffer does not include dNTPs (unlike HF buffer). Confirm dNTP inventory at order. dNTPs sold separately.
Confirm at quote: Hot Start chemistry evaluation intent (vs production-pack ordering), workflow application fit (multiplex / GC-rich / low-copy), production-pack interest (PR1000-HS-500 follow-on), and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris PR1000-HS Hot Start Taq family:
- PR1000-HS-S (this product): Sample, 50 units ($7.65), first-use evaluation
- PR1000-HS-500, 500 units ($232), small lab production pack
- PR1000-HS-1000, 1000 units, mid-throughput production pack
- PR1000-HS-6000, 6000 units, high-throughput bulk pack
Pick the sample for Hot Start chemistry evaluation; step to the 500 / 1000 / 6000-unit packs for production workflows.
