The PR1001-HS-S is the trial / sample-size SKU of the Accuris Hot Start Taq 2X master mix family — 5-reaction pack for first-use evaluation of the Hot Start master mix format before committing to the larger production-scale packs. list price, the sample SKU enables labs to validate the Hot Start master mix performance on their specific PCR target — particularly important for the multiplex / GC-rich / low-copy / primer-dimer-prone workflows where Hot Start is the chemistry of choice.
Key Specifications
| SKU | PR1001-HS-S |
| Type | Sample / trial pack — Accuris Hot Start Taq Master Mix |
| Pack quantity | 5 reactions |
| PCR mode | Hot Start Taq — antibody-mediated activation |
| Format | Single-tube 2X master mix — polymerase + buffer + dNTPs + MgCl₂ + hot-start antibody chemistry in single tube |
| Sister sample SKUs | PR1001-HSR-S (Hot Start + Red Dye), PR1000-HS-S (standalone polymerase 50 units sample) |
| Production-scale follow-on | PR1001-HS-200, PR1001-HS-1000 |
Three Hot Start Sample SKUs — Three Workflow Evaluation Paths
The Accuris Hot Start chemistry has three matched-price sample SKUs, each evaluating a different workflow:
| Sample SKU | Format | Best for evaluation of |
| PR1000-HS-S | Standalone polymerase (50 units / ~25–50 rxn) | Hot Start chemistry + multi-tube setup workflow |
| PR1001-HS-S (this product) | Master mix (5 rxn) | Hot Start chemistry + single-tube convenience workflow |
| PR1001-HSR-S | Master mix + Red Dye (5 rxn) | Hot Start chemistry + single-tube convenience + direct-gel-load workflow |
Procurement strategy. Labs new to Accuris Hot Start chemistry can order all three Hot Start samples for a complete Hot Start workflow evaluation across formats (standalone vs master mix) and dye variants (no Red Dye vs Red Dye).
Per Brochure Hot Start Master Mix Applications
Per Accuris Life Science Reagents Catalog, Hot Start Taq Master Mix applications include:
- Colony PCR — when colony-screen primer pairs are prone to non-specifics
- Fast PCR — Hot Start prevents pre-activation extension during rapid setup
- GC-rich DNA amplification — Hot Start handles GC-rich secondary structures
- Low Copy PCR — Hot Start preserves sensitivity by eliminating low-template non-specifics
- Multiplex PCR — primary Hot Start application; multiple primer pairs benefit from controlled activation
Application Profile
Hot Start master mix first-time evaluation — labs evaluating the PR1001-HS master mix format for the first time use the sample to validate fit before bulk procurement.
Master mix vs standalone Hot Start comparison — labs comparing master mix convenience vs standalone cost-optimization use both PR1001-HS-S and PR1000-HS-S samples for the comparison.
Hot Start vs Hot Start + Red Dye comparison — labs comparing the two master mix variants use both PR1001-HS-S and PR1001-HSR-S samples for the direct-gel-load workflow comparison.
Multiplex assay validation — labs developing multiplex assays validate Hot Start master mix performance on the multiplex panel before scaling to production.
Sales / training samples — sales reps and training programs use the sample for hands-on customer demonstrations.
Educational laboratory PCR exercises — molecular-biology coursework introducing hot-start master mix concepts.
Ordering & Configuration
Single-use sample SKU. The 5-reaction pack supports a small validation run; not intended for routine production use.
Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂ + hot-start chemistry; operator supplies template, primers, and water.
Confirm at quote: Hot Start master mix evaluation intent (vs PR1001-HS-200 / -1000 production-pack ordering), workflow application fit (multiplex / GC-rich / low-copy), comparison to standalone (PR1000-HS-S sample) or Red Dye variant (PR1001-HSR-S sample), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris Hot Start Taq sample family:
- PR1000-HS-S — Standalone Hot Start polymerase, 50 units (multi-tube setup format)
- PR1001-HS-S (this product) — Hot Start Master Mix, 5 reactions (single-tube format)
- PR1001-HSR-S — Hot Start Master Mix + Red Dye, 5 reactions (single-tube + direct-gel-load)
Pick the PR1001-HS-S sample for Hot Start master mix evaluation; pair with sister samples for format / dye-variant comparison.
