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Accuris Taq DNA Polymerase, 1000 Units - End Point PCR Reagents | Legacy Lab Supplies

Accuris Taq DNA Polymerase, 1000 Units

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The PR1000-1000 is the mid pack-size of the Accuris standard Taq DNA Polymerase family — 1,000 units of recombinant Taq DNA Polymerase at 5 U/μL for endpoint PCR workflows.
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SKU: PR1000-1000

The PR1000-1000 is the mid pack-size of the Accuris standard Taq DNA Polymerase family — 1,000 units of recombinant Taq DNA Polymerase at 5 U/μL for endpoint PCR workflows. Each kit ships with 4 tubes of 5X buffer (containing PCR enhancers and 15 mM MgCl₂); dNTPs are sold separately. The PR1000 family is Accuris's standard (non-hot-start) Taq — appropriate for routine endpoint PCR where the lab does not require antibody-mediated hot-start activation (for hot-start workflows, see the PR1000-HS family).

Key Specifications

SKUPR1000-1000
TypeStandard Taq DNA Polymerase (non-hot-start)
Quantity1,000 units (200 μL at 5 U/μL)
Polymerase concentration5 U/μL
Included buffer4 tubes of 5X buffer with PCR enhancers and 15 mM MgCl₂
dNTPsNot included — available separately
PCR modeEndpoint PCR (standard Taq, not hot-start)

PR1000 Pack-Size Family

SKUQuantityUse case
PR1000-500500 unitsSmall lab / occasional PCR — entry pack
PR1000-1000 (this product)1,000 unitsMid-throughput / standard lab — workhorse pack
PR1000-60006,000 unitsHigh-throughput / production-scale lab

Pick PR1000-1000 when: the lab runs moderate-throughput endpoint PCR — typical academic-research lab consuming 1,000 units across a few weeks of project PCR, clinical-research lab running mid-volume diagnostic PCR, or teaching lab supporting a semester's worth of PCR coursework. Step up to PR1000-6000 for production-throughput; step down to PR1000-500 for occasional / pilot use.

Standard vs Hot-Start Taq Decision

FeaturePR1000 Standard Taq (this family)PR1000-HS Hot-Start Taq
ActivationActive at all temperatures (no hot-start step)Inactive below ~95 °C; activated by initial denaturation step
Setup workflowMix on ice; can be assembled at room temperature with careSetup at any temperature — antibody blocks polymerase activity until activation
Non-specific amplificationMore susceptible (extension at low temperatures during setup)Reduced — antibody-mediated activation suppresses pre-activation extension
Best fitRoutine endpoint PCR with well-optimized protocolsSensitive PCR (low template, multiplex, GC-rich, primer-dimer-prone)
CostStandardPremium

Pick standard Taq (PR1000) for: routine, well-optimized endpoint PCR workflows where hot-start premium is not justified by the workflow's sensitivity requirements.

Application Profile

Per the Accuris reagent catalog, standard Taq DNA Polymerase applications include:

  • Colony PCR — direct PCR from bacterial colony for clone screening
  • Fast PCR — short-amplicon PCR with reduced cycle times
  • Genotyping — endpoint PCR for SNP and indel genotyping workflows
  • High-throughput PCR — production-scale endpoint PCR for screening or diagnostics
  • Routine PCR — any general-purpose endpoint amplification workflow
  • Teaching laboratories — undergraduate and graduate molecular-biology coursework PCR exercises

Ordering & Configuration

dNTPs sold separately. The PR1000 kit includes the polymerase + 5X buffer; dNTPs must be purchased separately (Accuris offers dNTP mixes — confirm preferred catalog SKU at order). Plan procurement to include both reagents.

5X buffer with 15 mM MgCl₂. The included buffer brings PCR reactions to 1X with 3 mM MgCl₂ at the standard buffer dilution. Workflows requiring different MgCl₂ concentrations may need supplemental MgCl₂ (separate reagent).

Storage. Standard PCR enzyme storage applies — confirm storage protocol at order (typically -20 °C; check current Accuris product insert for specifics).

Confirm at quote: workflow throughput (drives pack-size selection), hot-start vs standard preference, dNTP / supplemental reagent procurement, storage capacity, and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote. Reagent batch lot numbers and expiration dates are documented per ship.

Accuris PR1000 Taq family:

- PR1000-500 — 500 units (small pack)

- PR1000-1000 (this product) — 1,000 units (workhorse pack)

- PR1000-6000 — 6,000 units (high-throughput pack)

- PR1000-HS-* — Hot-start Taq family (antibody-mediated hot-start activation; sister product line)

Pick the standard Taq pack size matching workflow throughput; step to PR1000-HS for hot-start chemistry.

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