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The PR1000-1000 is the mid pack-size of the Accuris standard Taq DNA Polymerase family, 1,000 units of recombinant Taq DNA Polymerase at 5 U/μL for endpoint PCR workflows. Each kit ships with 4 tubes of 5X buffer (containing PCR enhancers and 15 mM MgCl₂); dNTPs are sold separately. The PR1000 family is Accuris's standard (non-hot-start) Taq, appropriate for routine endpoint PCR where the lab does not require antibody-mediated hot-start activation (for hot-start workflows, see the PR1000-HS family).
| SKU | PR1000-1000 |
| Type | Standard Taq DNA Polymerase (non-hot-start) |
| Quantity | 1,000 units (200 μL at 5 U/μL) |
| Polymerase concentration | 5 U/μL |
| Included buffer | 4 tubes of 5X buffer with PCR enhancers and 15 mM MgCl₂ |
| dNTPs | Not included (available separately) |
| PCR mode | Endpoint PCR (standard Taq, not hot-start) |
| SKU | Quantity | Use case |
| PR1000-500 | 500 units | Small lab / occasional PCR (entry pack) |
| PR1000-1000 (this product) | 1,000 units | Mid-throughput / standard lab (workhorse pack) |
| PR1000-6000 | 6,000 units | High-throughput / production-scale lab |
Pick PR1000-1000 when: the lab runs moderate-throughput endpoint PCR, typical academic-research lab consuming 1,000 units across a few weeks of project PCR, clinical-research lab running mid-volume diagnostic PCR, or teaching lab supporting a semester's worth of PCR coursework. Step up to PR1000-6000 for production-throughput; step down to PR1000-500 for occasional / pilot use.
| Feature | PR1000 Standard Taq (this family) | PR1000-HS Hot-Start Taq |
| Activation | Active at all temperatures (no hot-start step) | Inactive below ~95 °C; activated by initial denaturation step |
| Setup workflow | Mix on ice; can be assembled at room temperature with care | Setup at any temperature (antibody blocks polymerase activity until activation) |
| Non-specific amplification | More susceptible (extension at low temperatures during setup) | Reduced (antibody-mediated activation suppresses pre-activation extension) |
| Best fit | Routine endpoint PCR with well-optimized protocols | Sensitive PCR (low template, multiplex, GC-rich, primer-dimer-prone) |
| Cost | Standard | Premium |
Pick standard Taq (PR1000) for: routine, well-optimized endpoint PCR workflows where hot-start premium is not justified by the workflow's sensitivity requirements.
Per the Accuris reagent catalog, standard Taq DNA Polymerase applications include:
dNTPs sold separately. The PR1000 kit includes the polymerase + 5X buffer; dNTPs must be purchased separately (Accuris offers dNTP mixes; confirm preferred catalog SKU at order). Plan procurement to include both reagents.
5X buffer with 15 mM MgCl₂. The included buffer brings PCR reactions to 1X with 3 mM MgCl₂ at the standard buffer dilution. Workflows requiring different MgCl₂ concentrations may need supplemental MgCl₂ (separate reagent).
Storage. Standard PCR enzyme storage applies. Confirm storage protocol at order (typically -20 °C; check current Accuris product insert for specifics).
Confirm at quote: workflow throughput (drives pack-size selection), hot-start vs standard preference, dNTP / supplemental reagent procurement, storage capacity, and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote. Reagent batch lot numbers and expiration dates are documented per ship.
Accuris PR1000 Taq family:
- PR1000-500: 500 units (small pack)
- PR1000-1000 (this product): 1,000 units (workhorse pack)
- PR1000-6000: 6,000 units (high-throughput pack)
- PR1000-HS-*: Hot-start Taq family (antibody-mediated hot-start activation; sister product line)
Pick the standard Taq pack size matching workflow throughput; step to PR1000-HS for hot-start chemistry.
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