The PR1001-1000 is the 1,000-reaction pack of the Accuris Taq 2X Master Mix — a pre-formulated single-tube 2× concentrated PCR master mix that combines Taq DNA Polymerase, buffer with PCR enhancers, dNTPs, and MgCl₂ in one tube. The master-mix format delivers significantly faster reaction setup vs the standalone PR1000 Taq + buffer + separate dNTPs workflow — combine equal volumes of master mix + (primers / template / water) and the reaction is ready to cycle. Ships as 20 × 1.25 mL tubes (1,000 reactions at 25 µL master mix per 50 µL reaction).
Key Specifications
| SKU | PR1001-1000 |
| Type | Taq 2X Master Mix — pre-formulated single-tube PCR master mix |
| Reactions per pack | 1,000 reactions (pack format: 20 × 1.25 mL tubes) |
| Concentration | 2× master mix (25 µL master mix + 25 µL primers/template/water = 50 µL standard reaction) |
| Polymerase | Taq DNA Polymerase (standard, non-hot-start) |
| Buffer composition | PCR enhancers, 2 mM dNTPs, 6 mM MgCl₂, inert red gel loading dye (optional) |
| dNTPs | INCLUDED — pre-formulated in master mix at 2 mM (1 mM final at 1× reaction concentration) |
| PCR mode | Endpoint PCR — standard Taq (not hot-start) |
| Smaller pack sister | PR1001-200 (200 reactions, 4 × 1.25 mL) |
| Red Dye sister variant | PR1001-R-1000 (with red gel-loading dye for direct gel-loading) |
| Hot Start sister variant | PR1001-HS-1000 (Hot Start Taq Master Mix) |
| Hot Start + Red Dye | PR1001-HSR-1000 |
| High Fidelity sister | PR1001-HF-200 / PR1001-HF-500 (HF Master Mix) |
| HF + Hot Start sister | PR1001-HFHS-200 / PR1001-HFHS-500 |
| Standalone enzyme sister | PR1000-1000 (Taq DNA Polymerase 1,000 units, separate buffer + dNTPs) |
| Returns | Non-returnable — perishable reagent per Accuris reagent-return policy |
Master Mix vs Standalone Enzyme — Workflow Decision
The same Taq DNA Polymerase ships in two procurement formats:
| Format | Workflow | When to pick |
| PR1001 Taq 2X Master Mix (this product's family) | Combine 25 µL master mix + 25 µL (primers / template / water) → ready to cycle | Routine PCR workflows where setup speed matters; standardized buffer + dNTP across reactions; reduced operator pipetting steps |
| PR1000 Taq DNA Polymerase (standalone enzyme) | Combine separate enzyme + 5X buffer + dNTPs + primers + template → ready to cycle | Custom-buffer workflows; specialty MgCl₂ adjustments; cost-controlled enzyme volume; dNTP customization |
The master-mix format is the right pick for routine PCR where setup speed matters and the standardized 6 mM MgCl₂ / 2 mM dNTP buffer is the workflow fit. Standalone enzyme is the right pick when custom buffer formulation matters (specialty templates, modified-base primers, optimization protocols).
Pack-Size Family — PR1001 Standard Master Mix
| SKU | Reactions | Pack format | Use case |
| PR1001-200 | 200 | 4 × 1.25 mL | Smaller pack — pilot work, occasional master-mix use, mid-cohort experiments |
| PR1001-1000 (this product) | 1,000 | 20 × 1.25 mL | Mid-throughput / production master-mix workflows |
The 20-tube format of the 1,000-reaction pack supports rotating tubes through active use while keeping unused tubes in deep-freeze storage — important for master-mix stability across the rotation period.
Reagent Composition
Per the Accuris Life Science Reagents Catalog, each PR1001-1000 pack includes:
- Taq DNA Polymerase (pre-formulated in 2× master mix)
- Buffer — PCR enhancers, 2 mM dNTPs, 6 mM MgCl₂
- Inert red gel loading dye (optional) — per the brochure formulation language; for explicit red-dye direct-loading, the PR1001-R variant is the workflow-appropriate SKU
Standard reaction setup: combine 25 µL of master mix + 25 µL of (primers + template + water) in a 50 µL reaction.
Variant Sister Family — When to Pick Which
The PR1001 family ships in eight variants addressing different chemistry / dye combinations:
| Variant | Hot Start? | Red Dye? | Fidelity | When to pick |
| PR1001 (this SKU's family) | No | No (or optional) | Standard | Routine endpoint PCR with master-mix convenience |
| PR1001-R | No | Yes | Standard | Routine PCR with direct gel-loading (no separate loading-dye step) |
| PR1001-HS | Yes | No | Standard | Multiplex / GC-rich / low-copy with master-mix convenience |
| PR1001-HSR | Yes | Yes | Standard | Hot Start + direct gel-loading combined |
| PR1001-HF | No | No | High | Cloning / sequencing / mutagenesis with master-mix convenience |
| PR1001-HFHS | Yes | No | High | Challenging templates needing both HF and Hot Start |
Application Profile (per Brochure)
Per the Accuris Life Science Reagents Catalog, Taq 2X Master Mix applications:
- Colony PCR — direct PCR from bacterial colony for clone screening
- Fast PCR — short-amplicon PCR with reduced cycle times
- Genotyping — endpoint PCR for SNP and indel genotyping
- High-throughput PCR — production-scale endpoint PCR for screening or diagnostics
- Routine PCR — general-purpose endpoint amplification
The brochure highlights master-mix performance with "High performance with gDNA & GC-rich sequences" — the 6 mM MgCl₂ / 2 mM dNTP formulation is tuned for routine PCR including modestly difficult templates.
When to Pick This Pack
Order PR1001-1000 when:
- Mid-throughput routine PCR — labs running ~50–200 PCR reactions per week where setup-speed and standardization matter
- Production endpoint PCR with standardized chemistry — production benches running endpoint PCR with consistent buffer / dNTP across reactions
- High-throughput screening — large-cohort screening workflows where master-mix setup speed reduces operator time
- Teaching laboratories — molecular-biology teaching where master-mix simplifies the student-side reaction setup
- Multi-bench shared inventory — institutional procurement of standardized PCR master mix across multiple benches
Ordering & Configuration
Storage & handling. Per Accuris kit instructions — typical PCR master-mix storage at −20 °C with limited freeze-thaw cycle tolerance. The 20-tube format supports rotating tubes through active use while keeping the rest in deep storage.
Loading-dye consideration. The base PR1001 includes inert red dye as "optional" per brochure language; for explicit direct-gel-loading workflow, the PR1001-R-1000 variant is the explicit Red-Dye SKU.
Hot-start vs standard verification. Confirm at order: routine on-ice setup (PR1001 standard is fine) vs room-temperature setup (use PR1001-HS-1000 instead). For challenging templates, consider PR1001-HF or PR1001-HFHS.
Returns. Per the universe price-list category header: reagent products including PCR master mixes are non-returnable due to perishable nature.
Confirm at quote: workflow throughput (200 vs 1,000 reaction pack), variant selection (standard / Red Dye / Hot Start / HF), workflow context, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
Accuris PR1001 Taq 2X Master Mix variant family:
- PR1001-200 / PR1001-1000 (this product) — Standard Taq Master Mix (200 / 1,000 rxn)
- PR1001-R-200 / PR1001-R-1000 — Red Dye Master Mix (direct gel-loading)
- PR1001-HS-200 / PR1001-HS-1000 — Hot Start Master Mix
- PR1001-HSR-200 / PR1001-HSR-1000 — Hot Start + Red Dye
- PR1001-HF-200 / PR1001-HF-500 — High Fidelity Master Mix
- PR1001-HFHS-200 / PR1001-HFHS-500 — HF + Hot Start combined
Pick by chemistry need (standard / hot-start / high-fidelity) and workflow ergonomics (Red Dye for direct gel-loading).
