Accuris Taq DNA Polymerase, 500 Units

The PR1000-500 is the smallest pack of the Accuris standard Taq DNA Polymerase family — 500 units of recombinant Taq DNA Polymerase at 5 U/μL for endpoint PCR workflows. The entry-pack format for research benches running occasional PCR, method-validation pilot work before committing to a larger procurement, or teaching-lab pilot exercises at smaller sample-cohort scale. Each kit ships with 1 tube of Taq DNA Polymerase (100 µL at 5 U/μL) + 4 tubes of 5X buffer containing PCR enhancers and 15 mM MgCl₂. dNTPs are sold separately.

Key Specifications

When to Pick the 500-Unit Pack

Order PR1000-500 when:

1. Pilot or method-validation work — validating a PCR protocol on a smaller enzyme volume before committing to a larger procurement
2. Occasional / low-volume PCR benches — research benches running PCR intermittently where 500 units matches a multi-month enzyme rotation
3. Teaching-lab pilot exercises — undergraduate or graduate teaching labs running pilot PCR cohorts (vs full-cohort exercises that would use the 1,000-unit or 6,000-unit pack)
4. Spare / backup enzyme inventory — supplemental procurement to ensure enzyme availability when the primary stock is rotating
5. Per-reaction cost not the primary procurement driver — when bench throughput doesn't justify the bulk-pack per-reaction economics

For mid-throughput labs, the PR1000-1000 (1,000-unit pack) is the workhorse pick at better per-reaction economics. For high-throughput labs, the PR1000-6000 bulk pack delivers the lowest per-reaction cost.

PR1000 Pack-Size Family — Per-Reaction Economics

Standard PCR reaction uses ~1.25 U Taq per 50 µL reaction (2.5 µL of 5 U/µL diluted in master mix); 500 units supports approximately 400 reactions at standard reaction volume.

Standard Taq vs Hot-Start Taq vs High-Fidelity Taq — Decision Matrix

The PR1000 family ships in three enzyme variants for different workflow requirements:

Pick standard Taq (PR1000) for: routine, well-optimized endpoint PCR workflows where the lab can reliably assemble reactions on ice and where the polymerase fidelity / hot-start premium is not justified.

Reagent Composition — What's Included

Per the Accuris Life Science Reagents Catalog ordering table, each PR1000-500 kit includes:

• 1 tube of Taq DNA Polymerase at 5 U/µL (100 µL volume = 500 units)
• 4 tubes of 5X buffer containing PCR enhancers and 15 mM MgCl₂

dNTPs are not included — order separately from the Accuris dNTP catalog (Life Science Reagents Catalog page 7) or supply from existing dNTP inventory. Plan procurement to include both reagents on the order.

Buffer Detail — 5X with 15 mM MgCl₂

The included 5X buffer brings reactions to 1X with 3 mM MgCl₂ at the standard buffer dilution. The buffer composition includes Accuris's PCR enhancer formulation (proprietary additives that improve performance with standard PCR templates).

For workflows requiring different MgCl₂ concentrations (e.g., difficult templates, GC-rich amplicons), supplemental MgCl₂ may be needed — order the supplemental MgCl₂ reagent separately or use the PR1001 master-mix family which includes pre-formulated MgCl₂ at 6 mM in the 2X master mix format.

Application Profile

Per the Accuris Life Science Reagents Catalog, standard Taq DNA Polymerase applications:

• Colony PCR — direct PCR from bacterial colony for clone screening
• Fast PCR — short-amplicon PCR with reduced cycle times
• Genotyping — endpoint PCR for SNP and indel genotyping workflows
• High-throughput PCR — production-scale endpoint PCR for screening or diagnostics
• Routine PCR — general-purpose endpoint amplification workflow
• Teaching laboratories — undergraduate and graduate molecular-biology coursework PCR exercises (the 500-unit pack is the right size for cohort exercises in the 50–400 reaction range)

Ordering & Configuration

dNTP procurement. dNTPs are sold separately; plan to include both PR1000-500 and a dNTP mix on the order. Confirm Accuris dNTP catalog SKU at order, or use existing dNTP inventory.

Storage & handling. Per Accuris kit instructions — typical Taq polymerase storage at −20 °C with limited freeze-thaw cycle tolerance.

Hot-start vs standard verification. Confirm at order: the workflow assembles reactions on ice (standard Taq is fine) vs at room temperature (use the PR1000-HS hot-start variant instead).

Returns. Per the universe price-list category header: reagent products including PCR enzymes are non-returnable due to perishable nature.

Confirm at quote: workflow throughput (drives pack-size selection — 500 vs 1,000 vs 6,000 units), enzyme variant (standard vs hot-start vs high-fidelity), dNTP procurement, shipping conditions (cold-chain), and lead time.

Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote. Reagent batch lot numbers and expiration dates are documented per ship.

Accuris PR1000 Taq family:

- PR1000-500 (this product) — Standard Taq, 500 units (smallest pack)

- PR1000-1000 — Standard Taq, 1,000 units (workhorse)

- PR1000-6000 — Standard Taq, 6,000 units (bulk)

- PR1000-HS-* — Hot-start Taq family

- PR1000-HF-* — High-fidelity Taq family

Pick the 500-unit pack for pilot work, occasional use, or teaching-lab exercises. Step up to 1,000 / 6,000 units for higher-throughput workflows. Step to PR1000-HS for hot-start chemistry or PR1000-HF for high-fidelity applications.