Accuris Taq DNA Polymerase, 500 Units

The PR1000-500 is the smallest pack of the Accuris standard Taq DNA Polymerase family, 500 units of recombinant Taq DNA Polymerase at 5 U/μL for endpoint PCR workflows. The entry-pack format for research benches running occasional PCR, method-validation pilot work before committing to a larger procurement, or teaching-lab pilot exercises at smaller sample-cohort scale. Each kit ships with 1 tube of Taq DNA Polymerase (100 µL at 5 U/μL) + 4 tubes of 5X buffer containing PCR enhancers and 15 mM MgCl₂. dNTPs are sold separately.

Key Specifications

When to Pick the 500-Unit Pack

Order PR1000-500 when:

1. Pilot or method-validation work. Validating a PCR protocol on a smaller enzyme volume before committing to a larger procurement
2. Occasional / low-volume PCR benches. Research benches running PCR intermittently where 500 units matches a multi-month enzyme rotation
3. Teaching-lab pilot exercises. Undergraduate or graduate teaching labs running pilot PCR cohorts (vs full-cohort exercises that would use the 1,000-unit or 6,000-unit pack)
4. Spare / backup enzyme inventory. Supplemental procurement to ensure enzyme availability when the primary stock is rotating
5. Per-reaction cost not the primary procurement driver. When bench throughput doesn't justify the bulk-pack per-reaction economics

For mid-throughput labs, the PR1000-1000 (1,000-unit pack) is the workhorse pick at better per-reaction economics. For high-throughput labs, the PR1000-6000 bulk pack delivers the lowest per-reaction cost.

PR1000 Pack-Size Family: Per-Reaction Economics

Standard PCR reaction uses ~1.25 U Taq per 50 µL reaction (2.5 µL of 5 U/µL diluted in master mix); 500 units supports approximately 400 reactions at standard reaction volume.

Standard Taq vs Hot-Start Taq vs High-Fidelity Taq: Decision Matrix

The PR1000 family ships in three enzyme variants for different workflow requirements:

Pick standard Taq (PR1000) for: routine, well-optimized endpoint PCR workflows where the lab can reliably assemble reactions on ice and where the polymerase fidelity / hot-start premium is not justified.

Reagent Composition: What's Included

Per the Accuris Life Science Reagents Catalog ordering table, each PR1000-500 kit includes:

• 1 tube of Taq DNA Polymerase at 5 U/µL (100 µL volume = 500 units)
• 4 tubes of 5X buffer containing PCR enhancers and 15 mM MgCl₂

dNTPs are not included. Order separately from the Accuris dNTP catalog (Life Science Reagents Catalog page 7) or supply from existing dNTP inventory. Plan procurement to include both reagents on the order.

Buffer Detail: 5X with 15 mM MgCl₂

The included 5X buffer brings reactions to 1X with 3 mM MgCl₂ at the standard buffer dilution. The buffer composition includes Accuris's PCR enhancer formulation (proprietary additives that improve performance with standard PCR templates).

For workflows requiring different MgCl₂ concentrations (e.g., difficult templates, GC-rich amplicons), supplemental MgCl₂ may be needed. Order the supplemental MgCl₂ reagent separately or use the PR1001 master-mix family which includes pre-formulated MgCl₂ at 6 mM in the 2X master mix format.

Application Profile

Per the Accuris Life Science Reagents Catalog, standard Taq DNA Polymerase applications:

• Colony PCR. Direct PCR from bacterial colony for clone screening
• Fast PCR. Short-amplicon PCR with reduced cycle times
• Genotyping. Endpoint PCR for SNP and indel genotyping workflows
• High-throughput PCR. Production-scale endpoint PCR for screening or diagnostics
• Routine PCR. General-purpose endpoint amplification workflow
• Teaching laboratories. Undergraduate and graduate molecular-biology coursework PCR exercises (the 500-unit pack is the right size for cohort exercises in the 50–400 reaction range)

Ordering & Configuration

dNTP procurement. dNTPs are sold separately; plan to include both PR1000-500 and a dNTP mix on the order. Confirm Accuris dNTP catalog SKU at order, or use existing dNTP inventory.

Storage & handling. Per Accuris kit instructions: typical Taq polymerase storage at −20 °C with limited freeze-thaw cycle tolerance.

Hot-start vs standard verification. Confirm at order: the workflow assembles reactions on ice (standard Taq is fine) vs at room temperature (use the PR1000-HS hot-start variant instead).

Returns. Per the universe price-list category header: reagent products including PCR enzymes are non-returnable due to perishable nature.

Confirm at quote: workflow throughput (drives pack-size selection; 500 vs 1,000 vs 6,000 units), enzyme variant (standard vs hot-start vs high-fidelity), dNTP procurement, shipping conditions (cold-chain), and lead time.

Warranty: Per Accuris Life Science Reagents standard kit warranty terms. Confirm at quote. Reagent batch lot numbers and expiration dates are documented per ship.

Accuris PR1000 Taq family:

- PR1000-500 (this product): Standard Taq, 500 units (smallest pack)

- PR1000-1000: Standard Taq, 1,000 units (workhorse)

- PR1000-6000: Standard Taq, 6,000 units (bulk)

- PR1000-HS-*: Hot-start Taq family

- PR1000-HF-*: High-fidelity Taq family

Pick the 500-unit pack for pilot work, occasional use, or teaching-lab exercises. Step up to 1,000 / 6,000 units for higher-throughput workflows. Step to PR1000-HS for hot-start chemistry or PR1000-HF for high-fidelity applications.