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The PR1001-200 is the single-tube 2X concentrated Taq master mix for routine endpoint PCR — sold as 200-reaction pack (4 × 1.25 mL tubes). Per the Accuris Life Science Reagents Catalog: *"Single-tube formulation: Ready to use 2X Master Mix."* The master mix integrates Taq polymerase + buffer + PCR enhancers + 2 mM dNTPs + 6 mM MgCl₂ + optional inert red gel loading dye into one tube — eliminating the multi-tube setup workflow of standalone polymerase + separate buffer + separate dNTPs that the PR1000 standalone Taq family requires.
| SKU | PR1001-200 |
| Type | Single-tube 2X Taq master mix |
| Pack quantity | 200 reactions (4 × 1.25 mL tubes) |
| PCR mode | Standard endpoint Taq (non-hot-start, non-HF) |
| Master mix composition | Taq DNA Polymerase + buffer with PCR enhancers + 2 mM dNTPs + 6 mM MgCl₂ + (optional) inert red gel loading dye for direct gel loading |
| Final concentration | 1X working in PCR (operator combines equal volume of 2X mix + template / primers / water) |
| Sister SKUs | PR1001-1000 (1000 reactions), PR1001-R-200 (Red Dye variant), PR1001-R-1000, PR1001-HS-200 (Hot Start variant), PR1001-HF-200 (HF variant) |
| List price | $94 |
The Accuris PR1000 standalone Taq family (PR1000-500 / -1000 / -6000) and the PR1001 master mix family (PR1001-200 / -1000) deliver the same PCR chemistry with different workflow profiles:
| Feature | PR1000 standalone Taq | PR1001 2X master mix (this product) |
| Tubes per reaction | 4+ tubes — Taq + buffer + dNTPs + (sometimes MgCl₂) | 1 tube — pre-mixed |
| Setup time | Multi-step pipetting per reaction setup | Single pipetting step of 2X mix into reaction tube |
| dNTPs | Sold separately (extra procurement line) | Included in the 2X mix |
| MgCl₂ | In the buffer at 15 mM (3 mM in 1X) | In the mix at 6 mM (3 mM in 1X) — same final concentration |
| Red dye for direct gel loading | Not included — separate loading dye purchase | Optional included in some variants (PR1001-R) |
| Per-reaction cost | Standalone components total ~$0.30–0.50 per reaction at production scale | $0.47 per reaction (PR1001-200 at $94) |
| Best fit | High-throughput labs that already have all components in stock; cost-optimized at scale | Convenience-prioritized workflows; reduced setup-error risk; teaching labs |
Pick the 2X master mix when: the workflow benefits from the single-tube setup convenience (faster setup, fewer pipetting errors, fewer procurement lines to manage) — particularly for teaching labs, multi-operator shared benches, and clinical-research labs where setup-consistency matters.
Pick the standalone Taq when: the lab is high-throughput, already manages dNTP inventory separately, and the per-reaction cost optimization matters at scale.
The PR1001 master mix family extends across all three polymerase chemistries:
| Master mix family | Chemistry | Pack sizes |
| PR1001-200 / -1000 (this product family) | Standard Taq 2X | 200 / 1000 reactions |
| PR1001-R-200 / -R-1000 | Standard Taq 2X + Red Dye for direct gel loading | 200 / 1000 reactions |
| PR1001-HS-200 / -HS-1000 | Hot Start Taq 2X | 200 / 1000 reactions |
| PR1001-HSR-200 / -HSR-1000 | Hot Start Taq 2X + Red Dye for direct gel loading | 200 / 1000 reactions |
| PR1001-HF-200 / -HF-500 | High Fidelity Master Mix | 200 / 500 reactions |
| PR1001-HFHS-200 / -HFHS-500 | High Fidelity Hot Start Master Mix | 200 / 500 reactions |
Pick PR1001-200 (standard Taq 2X) for: routine endpoint PCR, colony PCR, genotyping workflows where standard Taq chemistry is sufficient and the lab does not need direct-gel-load red dye.
Per brochure, the standard PR1001 master mix has optional inert red gel loading dye as part of the formulation. The PR1001-R sister SKU is the dedicated red-dye variant that enables direct gel loading without a separate loading-dye addition step — useful for high-throughput screening workflows where every saved pipetting step matters.
Standard PR1001-200 (this product): Master mix with optional dye flag — confirm with Accuris which formulation ships under the non-R suffix. PR1001-R-200 (sister SKU): Master mix with red dye explicitly included for direct gel loading.
Routine endpoint PCR — the workhorse application. Standard PCR for screening, verification, genotyping.
Colony PCR — direct PCR from bacterial colony for clone screening.
Fast PCR — short-amplicon PCR with reduced cycle times.
Genotyping — endpoint PCR for SNP and indel genotyping.
High-throughput PCR — production-scale screening workflows where the 200-reaction pack supports a meaningful workflow batch.
Teaching laboratory PCR — molecular-biology coursework benefits from the single-tube setup that simplifies student-cohort PCR exercises.
Clinical-research PCR — clinical-research labs running standardized endpoint PCR protocols benefit from the consistency of the master mix format.
Multi-operator shared benches — facilities where multiple operators run PCR benefit from the master-mix format's reduced setup-variability across operators.
Per-reaction workflow consumable. Each 25-µL PCR reaction uses 12.5 µL of 2X master mix. A 1.25 mL tube supports 100 reactions; the 200-reaction pack is 4 × 1.25 mL tubes.
Storage. Standard PCR enzyme storage applies — confirm storage protocol at order (typically -20 °C).
Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; the operator supplies template, primers, and water (or PCR-grade buffer for the dilution step).
Confirm at quote: workflow PCR throughput (drives 200-rxn vs 1000-rxn pack-size selection), chemistry preference (standard / Hot Start / HF), red-dye direct-gel-load workflow fit (PR1001 vs PR1001-R), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1001 standard Taq master mix family:
- PR1001-200 (this product) — Taq 2X Master Mix, 200 reactions ($94)
- PR1001-1000 — Taq 2X Master Mix, 1000 reactions
- PR1001-R-200 — Taq 2X Master Mix + Red Dye, 200 reactions (direct gel loading)
- PR1001-R-1000 — Taq 2X Master Mix + Red Dye, 1000 reactions
Pick the standard Taq 2X master mix for routine endpoint PCR convenience; step to the Red Dye variant for direct-gel-load workflows; step to Hot Start (PR1001-HS) or HF (PR1001-HF / -HFHS) master mixes for chemistry-specific workflows.
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