Accuris Taq 2X Master Mix, 200 Reactions

The PR1001-200 is the single-tube 2X concentrated Taq master mix for routine endpoint PCR, sold as 200-reaction pack (4 × 1.25 mL tubes). Per the Accuris Life Science Reagents Catalog: *"Single-tube formulation: Ready to use 2X Master Mix."* The master mix integrates Taq polymerase + buffer + PCR enhancers + 2 mM dNTPs + 6 mM MgCl₂ + optional inert red gel loading dye into one tube, eliminating the multi-tube setup workflow of standalone polymerase + separate buffer + separate dNTPs that the PR1000 standalone Taq family requires.

Key Specifications

Why a 2X Master Mix vs Standalone Taq

The Accuris PR1000 standalone Taq family (PR1000-500 / -1000 / -6000) and the PR1001 master mix family (PR1001-200 / -1000) deliver the same PCR chemistry with different workflow profiles:

Pick the 2X master mix when: the workflow benefits from the single-tube setup convenience (faster setup, fewer pipetting errors, fewer procurement lines to manage), particularly for teaching labs, multi-operator shared benches, and clinical-research labs where setup-consistency matters.

Pick the standalone Taq when: the lab is high-throughput, already manages dNTP inventory separately, and the per-reaction cost optimization matters at scale.

Master Mix Family: Standard Taq vs Hot Start vs High Fidelity

The PR1001 master mix family extends across all three polymerase chemistries:

Pick PR1001-200 (standard Taq 2X) for: routine endpoint PCR, colony PCR, genotyping workflows where standard Taq chemistry is sufficient and the lab does not need direct-gel-load red dye.

Inert Red Dye Variant: When to Step to PR1001-R

Per brochure, the standard PR1001 master mix has optional inert red gel loading dye as part of the formulation. The PR1001-R sister SKU is the dedicated red-dye variant that enables direct gel loading without a separate loading-dye addition step, useful for high-throughput screening workflows where every saved pipetting step matters.

Standard PR1001-200 (this product): Master mix with optional dye flag. Confirm with Accuris which formulation ships under the non-R suffix. PR1001-R-200 (sister SKU): Master mix with red dye explicitly included for direct gel loading.

Application Profile

Routine endpoint PCR. The workhorse application. Standard PCR for screening, verification, genotyping.

Colony PCR. Direct PCR from bacterial colony for clone screening.

Fast PCR. Short-amplicon PCR with reduced cycle times.

Genotyping. Endpoint PCR for SNP and indel genotyping.

High-throughput PCR. Production-scale screening workflows where the 200-reaction pack supports a meaningful workflow batch.

Teaching laboratory PCR. Molecular-biology coursework benefits from the single-tube setup that simplifies student-cohort PCR exercises.

Clinical-research PCR. Clinical-research labs running standardized endpoint PCR protocols benefit from the consistency of the master mix format.

Multi-operator shared benches. Facilities where multiple operators run PCR benefit from the master-mix format's reduced setup-variability across operators.

Ordering & Configuration

Per-reaction workflow consumable. Each 25-µL PCR reaction uses 12.5 µL of 2X master mix. A 1.25 mL tube supports 100 reactions; the 200-reaction pack is 4 × 1.25 mL tubes.

Storage. Standard PCR enzyme storage applies. Confirm storage protocol at order (typically -20 °C).

Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; the operator supplies template, primers, and water (or PCR-grade buffer for the dilution step).

Confirm at quote: workflow PCR throughput (drives 200-rxn vs 1000-rxn pack-size selection), chemistry preference (standard / Hot Start / HF), red-dye direct-gel-load workflow fit (PR1001 vs PR1001-R), and lead time.

Warranty: Per Accuris standard reagent terms. Confirm at quote.

Accuris PR1001 standard Taq master mix family:

- PR1001-200 (this product): Taq 2X Master Mix, 200 reactions ($94)

- PR1001-1000: Taq 2X Master Mix, 1000 reactions

- PR1001-R-200: Taq 2X Master Mix + Red Dye, 200 reactions (direct gel loading)

- PR1001-R-1000: Taq 2X Master Mix + Red Dye, 1000 reactions

Pick the standard Taq 2X master mix for routine endpoint PCR convenience; step to the Red Dye variant for direct-gel-load workflows; step to Hot Start (PR1001-HS) or HF (PR1001-HF / -HFHS) master mixes for chemistry-specific workflows.