The PR1100-S is the trial / sample-size SKU of the Accuris One-Step RT-PCR Kit family — 10-reaction pack for first-use evaluation of the One-Step RT-PCR workflow before committing to the larger production-scale packs (PR1100-50 at $304 / PR1100-100 at $549). At $65 list price (vs the $7.65 standard polymerase-sample pricing pattern), the RT-PCR sample reflects the cost of the thermostable RTase enzyme and RNase inhibitor inclusion that the standard PR1000 / PR1001 polymerase samples don't include.
Key Specifications
| SKU | PR1100-S |
| Type | Sample / trial pack — Accuris One-Step RT-PCR Kit |
| Pack quantity | 10 reactions |
| Workflow | Single-tube cDNA synthesis + PCR amplification (One-Step RT-PCR) |
| Kit contents (proportional sample size) | Thermostable RTase + RNase inhibitor + 2X One-Step Mix (PCR enhancers, dNTPs, MgCl₂) + Hot Start Taq |
| Per-reaction cost (sample) | $6.50 |
| Production-scale follow-on | PR1100-50 ($304, $6.08/rxn), PR1100-100 ($549, $5.49/rxn) |
| List price | $65 |
Why a 10-Reaction Sample (vs 5-Reaction Polymerase Samples)
The PR1100-S sample pack has double the reaction count of the standard PR1001 master mix samples (10 vs 5) — reflecting the complexity of RT-PCR protocol validation that often requires more reactions to optimize:
| Validation step | Reaction count typically needed |
| No-template control | 1 reaction (negative control) |
| Positive control template | 1 reaction (positive amplification confirmation) |
| Annealing temperature optimization | 3–5 reactions across temp gradient |
| Template input titration | 3–5 reactions across input dilution |
| Replicate confirmation | 2–3 reactions for biological / technical replicates |
A 10-reaction sample supports a meaningful subset of the above validation steps in a single protocol-validation run — sufficient to make an informed decision on whether to scale to PR1100-50 or PR1100-100.
Why the $65 vs $7.65 Sample-Pricing Differential
The standard Accuris polymerase samples (PR1000-S / -HS-S / -HF-S, PR1001 master mix samples) all price at $7.65. The PR1100 RT-PCR sample at $65 carries a meaningful premium reflecting:
| Cost driver | Standard polymerase sample | PR1100-S One-Step RT-PCR sample |
| Polymerase reagent | Standard or HF Taq | Hot Start Taq (premium) |
| Reverse transcriptase | Not included | Thermostable RTase included (premium reagent) |
| RNase inhibitor | Not included | RNase inhibitor included (premium reagent) |
| Reaction count | 5–50 reactions | 10 reactions |
| Per-reaction cost | $0.15–1.53 | $6.50 |
The $65 price reflects the multi-reagent kit composition; the per-reaction cost ($6.50) is consistent with the production-scale per-reaction cost (PR1100-50 at $6.08/rxn) — the sample provides a fair-cost validation opportunity rather than a deeply-discounted promotional pack.
Per Brochure One-Step RT-PCR Applications
Per Accuris Life Science Reagents Catalog, One-Step RT-PCR Kit applications include:
- Multiplex RT-PCR — multiple primer pairs in one tube for multi-target gene expression
- Gene expression analysis — endpoint-RT-PCR mRNA expression measurement
Sample-pack validation contexts:
- Confirming RT-PCR sensitivity on the lab's specific RNA target (low-copy detection)
- Testing multiplex primer-pair compatibility before scaling
- Validating gene-expression protocol design before committing to a 50- or 100-sample experiment
- Method-development workflow for downstream qPCR assay design
Application Profile
One-Step RT-PCR first-time evaluation — labs evaluating the Accuris One-Step RT-PCR kit for the first time use the sample to validate fit before bulk procurement.
Multiplex RT-PCR design validation — labs developing multiplex RT-PCR panels validate primer-pair compatibility on the sample before scaling.
Gene-expression protocol validation — labs designing gene-expression studies use the sample to confirm protocol works on the target mRNA before scaling to the experimental sample cohort.
Method-development RT-PCR — protocol-development workflows where RT-PCR primer design needs validation.
Sales / training samples — sales reps and training programs use the sample for hands-on customer demonstrations.
Educational laboratory RT-PCR exercises — teaching labs introducing RT-PCR concepts on a per-class budget.
Ordering & Configuration
Single-use sample SKU. The 10-reaction pack supports a meaningful validation run; not intended for routine production use.
Pair with RNA template + sequence-specific primers. The kit includes the polymerase + RT + buffer + dNTPs + RNase inhibitor; operator supplies the RNA template and the gene-specific PCR primers.
Storage. RT-PCR reagent storage applies — typically -20 °C; RNase-free handling required.
Confirm at quote: One-Step RT-PCR evaluation intent (vs PR1100-50 / -100 production-pack ordering), workflow application (gene expression vs pathogen detection vs method-development), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1100 One-Step RT-PCR kit family:
- PR1100-S (this product) — Sample, 10 reactions ($65, $6.50/rxn)
- PR1100-50 — One-Step RT-PCR Kit, 50 reactions ($304, $6.08/rxn)
- PR1100-100 — One-Step RT-PCR Kit, 100 reactions ($549, $5.49/rxn)
Pick the sample for One-Step RT-PCR evaluation; step to the 50- or 100-rxn pack for production workflows.
