Accuris One-Step RT-PCR Kit, 50 Reactions

The Accuris One-Step RT-PCR Kit is a single-tube cDNA synthesis + PCR amplification kit for RNA → DNA → amplification workflows. The PR1100-50 is the 50-reaction pack — supports a meaningful validation or routine workflow batch. Per the Accuris Life Science Reagents Catalog: *"Convenient cDNA synthesis and subsequent PCR in a single tube."* The kit's chemistry is optimized for low-copy transcripts — the workflow is sensitive enough to detect dilute mRNA inputs without separate cDNA synthesis + PCR setup steps. Hot Start Taq Polymerase is included to prevent the non-specific amplification that two-step RT-PCR workflows typically suffer from at the PCR-setup transition.

Key Specifications

Why One-Step RT-PCR — Workflow Convenience + Sensitivity

Traditional two-step RT-PCR workflow:

1. cDNA synthesis — RNA + RTase + buffer + dNTPs + primer (random hexamer or oligo-dT) in tube #1, ~1-hr incubation at RT-optimal temperature
2. Sample transfer — operator transfers cDNA from tube #1 to tube #2
3. PCR amplification — cDNA + Taq + buffer + dNTPs + sequence-specific primers in tube #2, thermal cycling

One-step workflow (this kit) collapses all three into one tube: all reagents — RTase, Hot Start Taq, RNase inhibitor, buffer, dNTPs, primers, and RNA — are combined upfront, and the thermal cycler protocol drives both steps (an initial RT-temperature incubation for cDNA synthesis, then standard PCR cycling once the Hot Start activates the Taq). Because there is no tube transfer, the workflow eliminates the contamination, loss, and pipetting-error risk of the inter-step transfer.

Workflow advantages of one-step:

• Reduced contamination risk — closed-tube workflow reduces RNase contamination opportunities
• Reduced sample loss — no transfer step means no aspiration/dispensing loss
• Faster workflow — one tube setup vs two; one thermocycler run vs sequential RT then PCR
• Better low-copy sensitivity — entire RT product participates in PCR (no aliquot subsampling that two-step requires)

Hot Start Taq inclusion is critical for one-step success — without hot-start chemistry, the Taq would be active during the RT-temperature incubation and would generate non-specific PCR products from the as-synthesized cDNA before the formal PCR cycling begins.

PR1100 Pack-Size Family

Pick the PR1100-50 when: workflow throughput is moderate — typical clinical-research RT-PCR workflow consuming 50 reactions across a few weeks of project work, gene-expression studies running 50-sample panels, or method-development workflows beyond the 10-reaction sample scope. Step up to PR1100-100 for 100+ reactions per quarter; step down to PR1100-S for protocol validation.

Per Brochure One-Step RT-PCR Applications

Per the Accuris Life Science Reagents Catalog, One-Step RT-PCR Kit applications include multiplex RT-PCR (multiple primer pairs in one tube for multi-target gene-expression analysis) and gene-expression analysis (quantitative or semi-quantitative mRNA measurement at endpoint-RT-PCR scope; for fluorescence-based real-time RT-qPCR, see the qMAX RT-qPCR family).

Typical application contexts include pathogen detection (viral RNA detection, research-use only per kit positioning), gene-expression studies comparing target-gene mRNA levels across treatment and control conditions, mRNA-target validation confirming target presence in tissue or cell-line samples, and method-development RT-PCR for downstream qPCR assay design.

Application Profile

Routine gene expression analysis — the workhorse application. Endpoint RT-PCR for mRNA detection / semi-quantitative analysis.

Multiplex RT-PCR panels — multi-target gene expression measurement on a single sample input.

Pathogen RNA detection (research-use only) — viral RNA detection workflows in research labs.

Clinical-research mRNA workflows — clinical-research labs running RT-PCR for biomarker discovery or validation studies.

Method-development for downstream qPCR — protocol-development RT-PCR workflows that validate primer design before stepping to qMAX qPCR fluorescence chemistry.

Educational laboratory RT-PCR exercises — molecular-biology coursework introducing RT-PCR workflow.

Ordering & Configuration

Per-reaction workflow consumable. Each One-Step RT-PCR reaction consumes proportional reagent from the included tubes. The 50-reaction pack supports 50 RT-PCR reactions before reorder.

Storage. RT-PCR reagent storage applies — typically -20 °C; thermostable RTase storage protocol per product insert. RNase-free handling required throughout.

Pair with RNA template + sequence-specific primers. The kit includes the polymerase + RT + buffer + dNTPs + RNase inhibitor; operator supplies the RNA template and the gene-specific PCR primers (for one-step RT-PCR, the same primer pair drives both the RT step and the PCR step — no separate random hexamer / oligo-dT priming).

Confirm at quote: workflow throughput (drives 50-rxn vs 100-rxn pack-size selection), evaluation interest (PR1100-S sample at $65 for first-use validation), workflow application (gene expression vs pathogen detection vs method-development), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR1100 One-Step RT-PCR kit family:

- PR1100-S — Sample, 10 reactions ($65)

- PR1100-50 (this product) — One-Step RT-PCR Kit, 50 reactions ($304)

- PR1100-100 — One-Step RT-PCR Kit, 100 reactions ($549)

Pick the 50-reaction pack for moderate-throughput RT-PCR workflows; step to 100-rxn for higher throughput or sample for protocol validation.