The PR1001-HSR-S is the trial / sample-size SKU of the Accuris Hot Start Taq + Red Dye 2X master mix family, 5-reaction pack for first-use evaluation of the Hot Start + direct-gel-load combined workflow before committing to the larger production-scale packs. list price, the sample SKU enables labs to validate the combined Hot Start + Red Dye workflow on their specific multiplex / GC-rich / low-copy PCR target.
Key Specifications
| SKU | PR1001-HSR-S |
| Type | Sample / trial pack (Accuris Hot Start Taq + Red Dye Master Mix) |
| Pack quantity | 5 reactions |
| PCR mode | Hot Start Taq (antibody-mediated activation) |
| Format | Single-tube 2X master mix with Red Dye for direct gel loading |
| Workflow | Combined Hot Start specificity + direct-gel-load convenience |
| Sister sample SKU | PR1001-HS-S |
| Production-scale follow-on | PR1001-HSR-200, PR1001-HSR-1000 |
Hot Start Sample Pair: Workflow Comparison
The two PR1001 Hot Start sample SKUs each enable a direct workflow comparison on the lab's specific PCR target:
| Sample SKU | Master mix variant | Workflow advantage |
| PR1001-HS-S | No Red Dye | Hot Start chemistry; PCR product compatible with downstream sequencing / cloning / fluorescence detection (separate loading-dye step required for gel) |
| PR1001-HSR-S (this product) | + Red Dye | Hot Start chemistry + direct gel loading workflow. It eliminates separate loading-dye addition step |
Procurement strategy. Labs deciding between the two Hot Start master mix variants can order both samples for a direct workflow comparison. Confirm whether the Red Dye direct-gel-load convenience justifies the workflow lock-in, or whether the no-dye variant's downstream-workflow flexibility is preferred.
Per Brochure Hot Start + Red Dye Applications
Per Accuris Life Science Reagents Catalog, Hot Start Red Dye Master Mix supports:
- Multiplex PCR with gel readout. The workhorse application
- Colony PCR with primer-dimer-prone setups
- Fast PCR with rapid setup
- GC-rich DNA amplification with direct gel readout
- Low Copy PCR with direct gel readout
Application Profile
Hot Start Red Dye master mix workflow first-time evaluation. Labs evaluating the combined chemistry + workflow for the first time use the sample to validate fit before bulk procurement.
Multiplex PCR + direct-gel-load validation. Labs developing multiplex PCR panels with gel readout validate that the Red Dye workflow supports the multiplex protocol before scaling.
Hot Start vs Hot Start + Red Dye comparison. Labs comparing the two PR1001-HS variants use both samples (PR1001-HS-S + PR1001-HSR-S) for the direct comparison.
Sales / training samples. Sales reps and training programs use the sample for hands-on customer demonstrations of the combined Hot Start + direct-gel-load workflow.
Educational laboratory PCR exercises. Molecular-biology coursework demonstrating both hot-start chemistry and direct-gel-load workflow concepts in a single reagent.
Ordering & Configuration
Single-use sample SKU. The 5-reaction pack supports a small validation run; not intended for routine production use.
Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂ + Red Dye + hot-start chemistry; operator supplies template, primers, and water.
Confirm at quote: combined Hot Start + Red Dye workflow evaluation intent (vs PR1001-HSR-200 / -1000 production-pack ordering), comparison to no-dye variant (PR1001-HS-S sample for parallel validation), downstream workflow Red Dye compatibility, and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris PR1001 Hot Start sample pair:
- PR1001-HS-S: Hot Start Master Mix sample, 5 reactions
- PR1001-HSR-S (this product): Hot Start Master Mix + Red Dye sample, 5 reactions
Pick the PR1001-HSR-S sample for combined Hot Start + direct-gel-load workflow evaluation; pair with PR1001-HS-S for direct workflow comparison.
