The PR1001-HSR-200 is the Hot Start Taq 2X Master Mix WITH Red Dye — sold as 200-reaction pack (4 × 1.25 mL tubes) per the Accuris Life Science Reagents Catalog. Same Hot Start chemistry as the PR1001-HS-200 sister (antibody-mediated activation) PLUS inert red gel loading dye explicitly included for direct gel loading without a separate loading-dye addition step. Same $174 list price as PR1001-HS-200 — the Red Dye carries no premium; the choice is purely a workflow-preference decision.
For complete Hot Start chemistry rationale, applications (multiplex / GC-rich / low-copy / primer-dimer-prone), and per-reaction cost economics, see the PR1001-HS-200 sister page.
Key Specifications
| SKU | PR1001-HSR-200 |
| Type | Single-tube 2X Hot Start Taq master mix with inert red gel loading dye |
| Pack quantity | 200 reactions (4 × 1.25 mL tubes) |
| PCR mode | Hot Start Taq — antibody-mediated activation |
| Master mix composition | Taq DNA Polymerase + buffer with PCR enhancers + 2 mM dNTPs + 6 mM MgCl₂ + inert red gel loading dye (explicitly included) + hot-start antibody chemistry |
| Workflow advantage | Direct gel loading — no separate loading-dye addition step |
| Per-reaction cost | $0.87 per reaction (same as PR1001-HS-200 standard variant) |
| Standard sister | PR1001-HS-200 (200 rxn, $174 — same price, no Red Dye) |
| Larger pack | PR1001-HSR-1000 (1000 reactions, $710) |
| Sample | PR1001-HSR-S (5 reactions, $7.65) |
| List price | $174 |
When to Pick HSR (Red Dye) vs HS (No Dye)
| Downstream workflow | Red Dye compatible? | Recommended SKU |
| Direct gel loading and analysis | Yes — primary use case | PR1001-HSR-200 (this product) |
| High-throughput multiplex screening with gel readout | Yes | PR1001-HSR-200 |
| Endpoint multiplex PCR + gel doc | Yes | PR1001-HSR-200 |
| PCR for downstream sequencing | No — dye may interfere with sequencing chemistry | PR1001-HS-200 (no dye) |
| PCR for downstream cloning | No — dye may interfere with ligation efficiency | PR1001-HS-200 (no dye) |
| PCR for downstream fluorescence detection | No | PR1001-HS-200 (no dye) |
Mixed-workflow facilities may stock both variants. The price-equivalence makes dual-stocking economically reasonable.
Hot Start Master Mix Family — Pack-Size Economics (HSR)
| Pack | Reactions | List price | Per-reaction cost |
| PR1001-HSR-S | 5 | $7.65 | $1.53 |
| PR1001-HSR-200 (this product) | 200 | $174 | $0.87 |
| PR1001-HSR-1000 | 1000 | $710 | $0.71 |
Pick the 200-rxn pack when: workflow throughput is 200–1,000 Hot Start Red Dye reactions per quarter; step to the 1000-rxn pack for higher throughput where the per-reaction cost saving matters.
Per Brochure Hot Start + Red Dye Applications
Per Accuris Life Science Reagents Catalog, Hot Start Red Dye Master Mix supports the same applications as standard Hot Start (Multiplex PCR, GC-rich amplification, Low Copy PCR, Colony PCR, Fast PCR) plus the direct-gel-load workflow advantage.
Application Profile
High-throughput multiplex PCR screening with gel readout — the workhorse Hot Start Red Dye application. Multiplex panels for genotyping, pathogen-detection screening, or other multi-target endpoint workflows benefiting from Hot Start specificity AND direct-gel-load convenience.
Production-scale colony PCR screening — colony PCR with multiplex primer-pairs benefits from Hot Start non-specific suppression AND direct-gel-load throughput optimization.
Multi-operator screening benches — facilities where multiple operators run Hot Start multiplex screens benefit from both the master-mix format AND the Red Dye direct-gel-load workflow.
Routine endpoint multiplex PCR — labs running standardized multiplex protocols with gel readout benefit from the Red Dye workflow consistency.
Educational laboratory PCR exercises — teaching labs benefit from the simplified single-step workflow for student-cohort multiplex exercises.
Ordering & Configuration
Per-reaction workflow consumable. Same per-reaction usage as the standard PR1001-HS-200 — 12.5 µL of 2X master mix per PCR reaction.
Storage. Same Hot Start reagent storage as the no-dye variant — confirm storage protocol at order.
Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂ + Red Dye + hot-start chemistry; operator supplies template, primers, and water.
Confirm at quote: workflow direct-gel-load preference (PR1001-HSR vs PR1001-HS at same $174 — purely a workflow-preference decision), downstream workflow Red Dye compatibility, throughput sizing (200-rxn vs 1000-rxn pack), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1001-HSR Hot Start Red Dye master mix family:
- PR1001-HSR-S — Sample, 5 reactions ($7.65, $1.53/rxn)
- PR1001-HSR-200 (this product) — Hot Start Master Mix + Red Dye, 200 reactions ($174, $0.87/rxn)
- PR1001-HSR-1000 — Hot Start Master Mix + Red Dye, 1000 reactions ($710, $0.71/rxn)
Pick the Red Dye variant for direct-gel-load multiplex / GC-rich / low-copy workflows; step to PR1001-HS-200 (same $174) when downstream workflow requires dye-free PCR product.
