Accuris Hot Start Taq Master Mix Red Dye, 200 Reactions

The PR1001-HSR-200 is the Hot Start Taq 2X Master Mix WITH Red Dye, sold as 200-reaction pack (4 × 1.25 mL tubes) per the Accuris Life Science Reagents Catalog. Same Hot Start chemistry as the PR1001-HS-200 sister (antibody-mediated activation) PLUS inert red gel loading dye explicitly included for direct gel loading without a separate loading-dye addition step. Same $174 list price as PR1001-HS-200. The Red Dye carries no premium; the choice is purely a workflow-preference decision.

For complete Hot Start chemistry rationale, applications (multiplex / GC-rich / low-copy / primer-dimer-prone), and per-reaction cost economics, see the PR1001-HS-200 sister page.

Key Specifications

When to Pick HSR (Red Dye) vs HS (No Dye)

Mixed-workflow facilities may stock both variants. The price-equivalence makes dual-stocking economically reasonable.

Hot Start Master Mix Family: Pack-Size Economics (HSR)

Pick the 200-rxn pack when: workflow throughput is 200–1,000 Hot Start Red Dye reactions per quarter; step to the 1000-rxn pack for higher throughput where the per-reaction cost saving matters.

Per Brochure Hot Start + Red Dye Applications

Per Accuris Life Science Reagents Catalog, Hot Start Red Dye Master Mix supports the same applications as standard Hot Start (Multiplex PCR, GC-rich amplification, Low Copy PCR, Colony PCR, Fast PCR) plus the direct-gel-load workflow advantage.

Application Profile

High-throughput multiplex PCR screening with gel readout. The workhorse Hot Start Red Dye application. Multiplex panels for genotyping, pathogen-detection screening, or other multi-target endpoint workflows benefiting from Hot Start specificity AND direct-gel-load convenience.

Production-scale colony PCR screening. Colony PCR with multiplex primer-pairs benefits from Hot Start non-specific suppression AND direct-gel-load throughput optimization.

Multi-operator screening benches. Facilities where multiple operators run Hot Start multiplex screens benefit from both the master-mix format AND the Red Dye direct-gel-load workflow.

Routine endpoint multiplex PCR. Labs running standardized multiplex protocols with gel readout benefit from the Red Dye workflow consistency.

Educational laboratory PCR exercises. Teaching labs benefit from the simplified single-step workflow for student-cohort multiplex exercises.

Ordering & Configuration

Per-reaction workflow consumable. Same per-reaction usage as the standard PR1001-HS-200, 12.5 µL of 2X master mix per PCR reaction.

Storage. Same Hot Start reagent storage as the no-dye variant. Confirm storage protocol at order.

Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂ + Red Dye + hot-start chemistry; operator supplies template, primers, and water.

Confirm at quote: workflow direct-gel-load preference (PR1001-HSR vs PR1001-HS at same $174; purely a workflow-preference decision), downstream workflow Red Dye compatibility, throughput sizing (200-rxn vs 1000-rxn pack), and lead time.

Warranty: Per Accuris standard reagent terms. Confirm at quote.

Accuris PR1001-HSR Hot Start Red Dye master mix family:

- PR1001-HSR-S: Sample, 5 reactions ($7.65, $1.53/rxn)

- PR1001-HSR-200 (this product): Hot Start Master Mix + Red Dye, 200 reactions ($174, $0.87/rxn)

- PR1001-HSR-1000: Hot Start Master Mix + Red Dye, 1000 reactions ($710, $0.71/rxn)

Pick the Red Dye variant for direct-gel-load multiplex / GC-rich / low-copy workflows; step to PR1001-HS-200 (same $174) when downstream workflow requires dye-free PCR product.