Accuris Hot Start Taq Master Mix Red Dye, 1000 Reactions

The PR1001-HSR-1000 is the Hot Start Taq 2X Master Mix WITH Red Dye — sold as 1000-reaction pack (20 × 1.25 mL tubes) per the brochure. Same Hot Start chemistry as the PR1001-HS-1000 sister (antibody-mediated activation) PLUS inert red gel loading dye explicitly included in the master mix formulation for direct gel loading without a separate loading-dye addition step. Same $710 list price as PR1001-HS-1000 — the Red Dye variant carries no premium vs the standard variant; the choice between them is purely a workflow-preference decision.

For complete chemistry rationale, applications, and per-reaction cost economics, see the PR1001-HS-200 and PR1001-HS-1000 sister pages.

Key Specifications

Why Red Dye — Direct Gel Loading Workflow

The standard PCR-to-gel workflow has two pipetting steps after thermal cycling:

1. Add loading dye to PCR product (typical 1–2 µL of 6X loading dye per 10–20 µL PCR product)
2. Load mixture onto gel

The Red Dye master mix (PR1001-HSR family) eliminates step 1 — the loading dye is already in the PCR product because it was part of the 2X master mix from the start. After thermal cycling, the operator can load the PCR product directly onto the gel without a separate loading-dye addition step.

Workflow benefit:

• One fewer pipetting step per reaction → reduces per-reaction handling time
• Eliminates one source of pipetting error → improves workflow reproducibility
• Reduces consumable load → no separate loading-dye reagent + tip consumption
• High-throughput screening → meaningful when running 100+ PCR products per day

Trade-off: The Red Dye is inert during PCR — it does not affect the polymerase activity or PCR product integrity per Accuris design. However, some downstream workflows (sequencing, cloning, fluorescence-based detection) may not be compatible with PCR products containing the loading dye. Pick PR1001-HS (no Red Dye) when the PCR product feeds into downstream workflows that require dye-free template.

When to Pick HSR (Red Dye) vs HS (No Dye)

Mixed-workflow facilities may stock both variants — PR1001-HS for downstream-sensitive workflows + PR1001-HSR for direct-gel-load screening.

Application Profile

High-throughput screening multiplex PCR with gel readout — the workhorse Red Dye application. Daily 100+ PCR product workflows where eliminating the loading-dye addition step matters.

Production-scale colony PCR screening — colony screening pipelines where the throughput justifies workflow-step optimization.

Multi-operator screening benches — facilities where multiple operators run high-throughput PCR benefit from both the master-mix format AND the Red Dye direct-gel-load workflow.

Educational laboratory PCR exercises — teaching labs benefit from the simplified single-step workflow for student-cohort exercises.

Routine clinical-research PCR with gel readout — clinical-research labs running standardized PCR-to-gel workflows benefit from the Red Dye workflow consistency.

Per Brochure Hot Start + Red Dye Applications

Per Accuris Life Science Reagents Catalog, Hot Start Red Dye Master Mix supports the same applications as standard Hot Start (Multiplex PCR, GC-rich amplification, Low Copy PCR, Colony PCR, Fast PCR) plus the direct-gel-load workflow advantage.

Ordering & Configuration

Per-reaction workflow consumable. Same per-reaction usage as the standard PR1001-HS-1000 — 12.5 µL of 2X master mix per PCR reaction.

Storage. Same Hot Start reagent storage as the standard variant — confirm storage protocol at order.

Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂ + Red Dye; operator supplies template, primers, and water.

Confirm at quote: workflow direct-gel-load preference (PR1001-HSR vs PR1001-HS at same $710 — purely a workflow-preference decision), downstream workflow Red Dye compatibility, throughput sizing (1000-rxn vs 200-rxn pack), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR1001-HSR Hot Start Red Dye master mix family:

- PR1001-HSR-200 — Hot Start Master Mix + Red Dye, 200 reactions

- PR1001-HSR-1000 (this product) — Hot Start Master Mix + Red Dye, 1000 reactions ($710)

Pick the Red Dye variant for direct-gel-load workflows; step to PR1001-HS-1000 (same $710) when downstream workflow requires dye-free PCR product.