Accuris Hot Start Taq Master Mix, 200 Reactions

The PR1001-HS-200 is the single-tube 2X Hot Start Taq master mix — sold as 200-reaction pack (4 × 1.25 mL tubes) per the Accuris Life Science Reagents Catalog. Per brochure: *"Allows for reaction set-up at room temperature without the risk of non-specific amplification."* The Hot Start chemistry is antibody-mediated — the polymerase is inactive below ~95 °C and is activated by the initial denaturation step of the PCR cycle, eliminating pre-activation extension that hurts sensitivity in multiplex / GC-rich / low-copy / primer-dimer-prone workflows.

Key Specifications

Why Hot Start Master Mix vs Standard Taq Master Mix

Per brochure, the Hot Start master mix carries a $80 premium over the standard Taq master mix (PR1001-HS-200 at $174 vs PR1001-200 at $94 for the same 200-reaction pack). The premium is justified by hot-start chemistry's specific workflow benefits:

Pick Hot Start over standard Taq when: the workflow is multiplex, GC-rich, low-copy, or primer-dimer-prone — the $0.40 per-reaction premium is justified by reduced workflow failure rate and improved sensitivity / specificity.

Per Brochure Hot Start Master Mix Applications

Per Accuris Life Science Reagents Catalog, Hot Start Taq Master Mix applications include:

• Colony PCR — when colony-screen primer pairs are prone to non-specifics
• Fast PCR — Hot Start prevents pre-activation extension during rapid setup
• GC-rich DNA amplification — Hot Start handles GC-rich secondary structures
• Low Copy PCR — Hot Start preserves sensitivity by eliminating low-template non-specifics
• Multiplex PCR — primary Hot Start application; multiple primer pairs benefit from controlled activation

Hot Start Master Mix vs Hot Start Standalone Polymerase

The Accuris Hot Start chemistry is available in both formats:

Same chemistry, different workflow profile. Pick the master mix for setup convenience; pick the standalone for per-reaction cost optimization at scale.

When to Step to PR1001-HSR (Red Dye Variant)

The Accuris Hot Start master mix family also includes a Red Dye variant (PR1001-HSR) with inert red gel loading dye explicitly included for direct gel loading without a separate loading-dye addition step — useful for high-throughput screening workflows where every saved pipetting step matters.

Standard PR1001-HS-200 (this product): Hot Start master mix with optional dye flag — confirm formulation with Accuris. PR1001-HSR-200 (sister SKU): Hot Start master mix with red dye explicitly included for direct gel loading.

Application Profile

Multiplex PCR workflows — the workhorse Hot Start application. Multiple primer pairs in one reaction benefit from controlled activation.

GC-rich amplification — extreme-GC templates benefit from Hot Start chemistry over standard Taq.

Low-copy PCR — sensitive PCR from low template benefits from Hot Start sensitivity preservation.

Colony PCR with primer-dimer-prone setups — Hot Start eliminates the primer-dimer artifacts that plague standard Taq colony PCR.

Multi-operator shared benches — facilities where multiple operators run Hot Start PCR benefit from the master-mix format's reduced setup-variability across operators.

Teaching laboratory PCR introduction — molecular-biology coursework introducing hot-start chemistry concepts.

Ordering & Configuration

Per-reaction workflow consumable. Each PCR reaction uses 12.5 µL of the 2X master mix. A 1.25 mL tube supports 100 reactions; the 200-reaction pack is 4 × 1.25 mL tubes.

Storage. Hot Start reagent storage applies — confirm storage protocol at order.

Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; operator supplies template, primers, and water.

Confirm at quote: workflow Hot Start chemistry need, throughput (drives 200-rxn vs 1000-rxn pack-size selection), Red Dye direct-gel-load preference (PR1001-HS vs PR1001-HSR), evaluation interest (PR1001-HS-S sample for first-use validation), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR1001-HS Hot Start Taq master mix family:

- PR1001-HS-S — Sample, 5 reactions (~$7.65 per FQ-016 pricing pattern)

- PR1001-HS-200 (this product) — Hot Start Taq Master Mix, 200 reactions ($174)

- PR1001-HS-1000 — Hot Start Taq Master Mix, 1000 reactions ($710)

- PR1001-HSR-200 — Hot Start Taq Master Mix + Red Dye, 200 reactions

- PR1001-HSR-1000 — Hot Start Taq Master Mix + Red Dye, 1000 reactions ($710)

Pick the Hot Start master mix for multiplex / GC-rich / low-copy / primer-dimer workflows; step to PR1001-HSR for direct-gel-load workflows or PR1001-HFHS for combined HF + Hot Start chemistry.