Accuris Hot Start Taq Master Mix, 200 Reactions

The PR1001-HS-200 is the single-tube 2X Hot Start Taq master mix, sold as 200-reaction pack (4 × 1.25 mL tubes) per the Accuris Life Science Reagents Catalog. Per brochure: *"Allows for reaction set-up at room temperature without the risk of non-specific amplification."* The Hot Start chemistry is antibody-mediated. The polymerase is inactive below ~95 °C and is activated by the initial denaturation step of the PCR cycle, eliminating pre-activation extension that hurts sensitivity in multiplex / GC-rich / low-copy / primer-dimer-prone workflows.

Key Specifications

Why Hot Start Master Mix vs Standard Taq Master Mix

Per brochure, the Hot Start master mix carries a $80 premium over the standard Taq master mix (PR1001-HS-200 at $174 vs PR1001-200 at $94 for the same 200-reaction pack). The premium is justified by hot-start chemistry's specific workflow benefits:

Pick Hot Start over standard Taq when: the workflow is multiplex, GC-rich, low-copy, or primer-dimer-prone. The $0.40 per-reaction premium is justified by reduced workflow failure rate and improved sensitivity / specificity.

Per Brochure Hot Start Master Mix Applications

Per Accuris Life Science Reagents Catalog, Hot Start Taq Master Mix applications include:

• Colony PCR. When colony-screen primer pairs are prone to non-specifics
• Fast PCR. Hot Start prevents pre-activation extension during rapid setup
• GC-rich DNA amplification. Hot Start handles GC-rich secondary structures
• Low Copy PCR. Hot Start preserves sensitivity by eliminating low-template non-specifics
• Multiplex PCR. Primary Hot Start application; multiple primer pairs benefit from controlled activation

Hot Start Master Mix vs Hot Start Standalone Polymerase

The Accuris Hot Start chemistry is available in both formats:

Same chemistry, different workflow profile. Pick the master mix for setup convenience; pick the standalone for per-reaction cost optimization at scale.

When to Step to PR1001-HSR (Red Dye Variant)

The Accuris Hot Start master mix family also includes a Red Dye variant (PR1001-HSR) with inert red gel loading dye explicitly included for direct gel loading without a separate loading-dye addition step, useful for high-throughput screening workflows where every saved pipetting step matters.

Standard PR1001-HS-200 (this product): Hot Start master mix with optional dye flag. Confirm formulation with Accuris. PR1001-HSR-200 (sister SKU): Hot Start master mix with red dye explicitly included for direct gel loading.

Application Profile

Multiplex PCR workflows. The workhorse Hot Start application. Multiple primer pairs in one reaction benefit from controlled activation.

GC-rich amplification. Extreme-GC templates benefit from Hot Start chemistry over standard Taq.

Low-copy PCR. Sensitive PCR from low template benefits from Hot Start sensitivity preservation.

Colony PCR with primer-dimer-prone setups. Hot Start eliminates the primer-dimer artifacts that plague standard Taq colony PCR.

Multi-operator shared benches. Facilities where multiple operators run Hot Start PCR benefit from the master-mix format's reduced setup-variability across operators.

Teaching laboratory PCR introduction. Molecular-biology coursework introducing hot-start chemistry concepts.

Ordering & Configuration

Per-reaction workflow consumable. Each PCR reaction uses 12.5 µL of the 2X master mix. A 1.25 mL tube supports 100 reactions; the 200-reaction pack is 4 × 1.25 mL tubes.

Storage. Hot Start reagent storage applies. Confirm storage protocol at order.

Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; operator supplies template, primers, and water.

Confirm at quote: workflow Hot Start chemistry need, throughput (drives 200-rxn vs 1000-rxn pack-size selection), Red Dye direct-gel-load preference (PR1001-HS vs PR1001-HSR), evaluation interest (PR1001-HS-S sample for first-use validation), and lead time.

Warranty: Per Accuris standard reagent terms. Confirm at quote.

Accuris PR1001-HS Hot Start Taq master mix family:

- PR1001-HS-S: Sample, 5 reactions (~$7.65 per FQ-016 pricing pattern)

- PR1001-HS-200 (this product): Hot Start Taq Master Mix, 200 reactions ($174)

- PR1001-HS-1000: Hot Start Taq Master Mix, 1000 reactions ($710)

- PR1001-HSR-200: Hot Start Taq Master Mix + Red Dye, 200 reactions

- PR1001-HSR-1000: Hot Start Taq Master Mix + Red Dye, 1000 reactions ($710)

Pick the Hot Start master mix for multiplex / GC-rich / low-copy / primer-dimer workflows; step to PR1001-HSR for direct-gel-load workflows or PR1001-HFHS for combined HF + Hot Start chemistry.