Accuris Hot Start Taq Polymerase, 6,000 Units

The PR1000-HS-6000 is the 6,000-unit bulk pack of the Accuris Hot Start Taq Polymerase — the standalone enzyme variant used in custom-formulated PCR reactions. Hot Start Taq employs rapid antibody-mediated hot-start activation, which allows for reaction preparation at room temperature without the non-specific amplification risk that standard Taq carries when reactions are assembled at room temperature. Distinct from the Accuris qMAX RT-qPCR kits (PR2120 / PR2121 / PR2122 / PR2124 series) which include Hot Start Taq pre-formulated in master mix — this PR1000-HS-6000 is the standalone enzyme for labs running custom PCR formulations or workflows that supply their own buffer and dNTP mix.

Universe class flag: The universe price list classifies this SKU as capital, but a $1,933 enzyme pack at 6,000 units is clearly consumable. Page authored as consumable; flagging for post-launch class correction.

Key Specifications

What This Enzyme Does

Hot Start Taq Polymerase is a modified Taq DNA polymerase that prevents non-specific amplification during reaction setup at room temperature. Standard Taq is enzymatically active at room temperature; if reactions are assembled at room temperature (rather than on ice), the enzyme can extend mismatched primers and generate non-specific PCR products before the thermal cycling protocol's denaturation step. Hot Start Taq prevents this by:

1. Antibody-mediated hot-start activation — an inhibitory antibody binds to the polymerase and blocks its activity at room temperature
2. Heat-activated antibody release — at the cycler's initial denaturation step (typically 95 °C), the antibody dissociates from the enzyme, restoring full polymerase activity
3. Standard PCR cycling — once activated, Hot Start Taq performs identically to standard Taq through the rest of the PCR protocol

Result: reactions can be assembled at room temperature on the bench (improved workflow ergonomics, larger reaction-batch setup) without the non-specific amplification risk of standard Taq.

Standalone Enzyme vs Master Mix — When to Pick

The Accuris enzyme catalog offers Hot Start Taq in two procurement formats:

Pick the standalone enzyme when the workflow needs custom buffer formulation (specialty templates, modified-base primers, low-quality template sources) or when bulk-pack enzyme pricing economics drive lower per-reaction cost than master-mix formats.

PR1000 Enzyme Family

Pick PR1000-HS when the workflow benefits from room-temperature reaction setup; pick PR1000-HF when fidelity matters more than cost; pick PR1000 (standard) for routine PCR with on-ice reaction setup.

Pack-Size Family — PR1000-HS

The 6,000-unit bulk pack is the right pick for high-throughput PCR labs where bulk-pack per-unit economics deliver substantial savings over the smaller pack sizes.

Application Profile

High-throughput routine PCR workflows — institutional clinical-research, pharma R&D, and academic core facilities running daily PCR at high reaction volume.

Custom-formulated PCR workflows — specialty templates (low-input GC-rich, AT-rich, secondary-structured), specialty primer designs, low-quality template sources where custom buffer formulation matters.

Genotyping and variant-confirmation workflows — endpoint PCR for variant confirmation, restriction-fragment analysis, allele-specific PCR.

Educational and training labs — molecular biology curricula teaching PCR methodology benefit from the standalone enzyme for hands-on reaction-formulation teaching.

Bulk-pack institutional procurement — labs running >6,000 PCR reactions per pack-purchase cycle benefit from the bulk-pack per-unit economics.

Ordering & Configuration

Storage & handling. Per kit instructions — typical Taq polymerase storage at −20 °C with limited freeze-thaw cycle tolerance. Confirm storage and handling requirements at receipt per Accuris kit instructions.

Reaction-setup workflow. Hot Start Taq enables room-temperature reaction setup; confirm at order whether the lab's workflow benefits from room-temperature setup (vs traditional on-ice assembly).

Confirm at quote: PCR workflow context (standalone enzyme vs master-mix preference), expected pack-purchase cycle volume, shipping conditions (cold-chain), and lead time.

Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.

PR1000 enzyme family:

- PR1000-HS-6000 (this product) — Hot Start Taq, 6,000 units (bulk pack)

- PR1000-HS-1000 / PR1000-HS-500 / PR1000-HS-S — Hot Start Taq smaller pack sizes

- PR1000 — Standard Taq (no hot start)

- PR1000-HF — High Fidelity Taq

Pick the PR1000-HS-6000 for high-throughput PCR labs requiring Hot Start Taq at bulk-pack economics.