Accuris Hot Start Taq Polymerase, 6,000 Units

The PR1000-HS-6000 is the 6,000-unit bulk pack of the Accuris Hot Start Taq Polymerase, the standalone enzyme variant used in custom-formulated PCR reactions. Hot Start Taq employs rapid antibody-mediated hot-start activation, which allows for reaction preparation at room temperature without the non-specific amplification risk that standard Taq carries when reactions are assembled at room temperature. Distinct from the Accuris qMAX RT-qPCR kits (PR2120 / PR2121 / PR2122 / PR2124 series) which include Hot Start Taq pre-formulated in master mix. This PR1000-HS-6000 is the standalone enzyme for labs running custom PCR formulations or workflows that supply their own buffer and dNTP mix.

Universe class flag: The universe price list classifies this SKU as capital, but a $1,933 enzyme pack at 6,000 units is clearly consumable. Page authored as consumable; flagging for post-launch class correction.

Key Specifications

What This Enzyme Does

Hot Start Taq Polymerase is a modified Taq DNA polymerase that prevents non-specific amplification during reaction setup at room temperature. Standard Taq is enzymatically active at room temperature; if reactions are assembled at room temperature (rather than on ice), the enzyme can extend mismatched primers and generate non-specific PCR products before the thermal cycling protocol's denaturation step. Hot Start Taq prevents this by:

1. Antibody-mediated hot-start activation. An inhibitory antibody binds to the polymerase and blocks its activity at room temperature
2. Heat-activated antibody release. At the cycler's initial denaturation step (typically 95 °C), the antibody dissociates from the enzyme, restoring full polymerase activity
3. Standard PCR cycling. Once activated, Hot Start Taq performs identically to standard Taq through the rest of the PCR protocol

Result: reactions can be assembled at room temperature on the bench (improved workflow ergonomics, larger reaction-batch setup) without the non-specific amplification risk of standard Taq.

Standalone Enzyme vs Master Mix: When to Pick

The Accuris enzyme catalog offers Hot Start Taq in two procurement formats:

Pick the standalone enzyme when the workflow needs custom buffer formulation (specialty templates, modified-base primers, low-quality template sources) or when bulk-pack enzyme pricing economics drive lower per-reaction cost than master-mix formats.

PR1000 Enzyme Family

Pick PR1000-HS when the workflow benefits from room-temperature reaction setup; pick PR1000-HF when fidelity matters more than cost; pick PR1000 (standard) for routine PCR with on-ice reaction setup.

Pack-Size Family: PR1000-HS

The 6,000-unit bulk pack is the right pick for high-throughput PCR labs where bulk-pack per-unit economics deliver substantial savings over the smaller pack sizes.

Application Profile

High-throughput routine PCR workflows. Institutional clinical-research, pharma R&D, and academic core facilities running daily PCR at high reaction volume.

Custom-formulated PCR workflows. Specialty templates (low-input GC-rich, AT-rich, secondary-structured), specialty primer designs, low-quality template sources where custom buffer formulation matters.

Genotyping and variant-confirmation workflows. Endpoint PCR for variant confirmation, restriction-fragment analysis, allele-specific PCR.

Educational and training labs. Molecular biology curricula teaching PCR methodology benefit from the standalone enzyme for hands-on reaction-formulation teaching.

Bulk-pack institutional procurement. Labs running >6,000 PCR reactions per pack-purchase cycle benefit from the bulk-pack per-unit economics.

Ordering & Configuration

Storage & handling. Per kit instructions: typical Taq polymerase storage at −20 °C with limited freeze-thaw cycle tolerance. Confirm storage and handling requirements at receipt per Accuris kit instructions.

Reaction-setup workflow. Hot Start Taq enables room-temperature reaction setup; confirm at order whether the lab's workflow benefits from room-temperature setup (vs traditional on-ice assembly).

Confirm at quote: PCR workflow context (standalone enzyme vs master-mix preference), expected pack-purchase cycle volume, shipping conditions (cold-chain), and lead time.

Warranty: Per Accuris Life Science Reagents standard kit warranty terms. Confirm at quote.

PR1000 enzyme family:

- PR1000-HS-6000 (this product): Hot Start Taq, 6,000 units (bulk pack)

- PR1000-HS-1000 / PR1000-HS-500 / PR1000-HS-S: Hot Start Taq smaller pack sizes

- PR1000: Standard Taq (no hot start)

- PR1000-HF: High Fidelity Taq

Pick the PR1000-HS-6000 for high-throughput PCR labs requiring Hot Start Taq at bulk-pack economics.