The PR1000-HS-1000 is the workhorse mid-throughput pack of the Accuris Hot Start Taq DNA Polymerase family — 1,000 units of Hot Start Taq at 5 U/µL with antibody-mediated hot-start activation. Right-sized for benches running routine multiplex / GC-rich / low-copy / fast PCR at moderate-to-mid throughput where the 500-unit pack is too small for the workflow rotation and the 6,000-unit bulk pack over-stocks. Each kit ships with 1 tube of Hot Start Taq (200 µL at 5 U/µL) + 4 tubes of 5X buffer containing PCR enhancers and 15 mM MgCl₂. dNTPs are sold separately.
Key Specifications
| SKU | PR1000-HS-1000 |
| Type | Hot Start Taq DNA Polymerase — antibody-mediated hot-start activation |
| Quantity | 1,000 units (200 µL at 5 U/µL) |
| Polymerase concentration | 5 U/µL |
| Hot-start mechanism | Antibody-mediated — antibody blocks polymerase activity below ~95 °C, dissociates at initial denaturation step |
| Included buffer | 4 tubes of 5X buffer with PCR enhancers and 15 mM MgCl₂ |
| dNTPs | Not included — available separately |
| Smaller pack sister | PR1000-HS-500 (500 units) |
| Larger pack sister | PR1000-HS-6000 (6,000 units bulk) |
| Sample-size sister | PR1000-HS-S |
| Standard-Taq sister family | PR1000 (no hot-start, lower cost) |
| High-fidelity sister family | PR1000-HF |
| List price | $400 |
| Returns | Non-returnable — perishable reagent per Accuris reagent-return policy |
Pack-Size Per-Unit Economics
The PR1000-HS-1000 sits in the per-unit-cost middle of the Hot Start family:
| SKU | Units | List price | Per-unit cost | Per-unit savings vs PR1000-HS-500 |
| PR1000-HS-500 | 500 | $232 | $0.464 / unit | (baseline) |
| PR1000-HS-1000 (this product) | 1,000 | $400 | $0.400 / unit | ~14% lower |
| PR1000-HS-6000 | 6,000 | $1,933 | $0.322 / unit | ~31% lower |
The 1,000-unit pack is the workhorse pick for mid-throughput labs where the 14% per-unit savings vs the 500-unit pack matters but the workflow doesn't justify the 6,000-unit bulk pack. A 1,000-unit pack supports approximately ~800 standard PCR reactions at 1.25 units per 50 µL reaction — typical 2–3 month rotation for a moderate-throughput PCR bench.
When to Pick This Pack
Order the PR1000-HS-1000 for routine Hot Start PCR at moderate throughput — clinical-research, academic-research, or biotech labs running roughly 50–100 Hot Start PCR reactions per week. It is the natural primary stock for multiplex PCR (clinical-research panels, agricultural-genotyping multiplex, microbiome multiplex), for GC-rich amplicon workflows on high-GC bacterial/archaeal genomes, mammalian CpG-island regions, and secondary-structured templates, and for low-copy or rare-target PCR where any non-specific amplification competes with the specific signal. It also suits fast, short-amplicon cycling where Hot Start prevents mispriming during the rapid setup-to-cycling transition, and it is the standard step-up from the PR1000-HS-500 once a lab's throughput justifies the workhorse pack.
What Hot Start Taq Does
Hot Start Taq is chemically inhibited at room temperature by an inhibitory antibody bound to the polymerase active site. The antibody dissociates at the cycler's initial denaturation step (typically 95 °C for 2–5 minutes), restoring full polymerase activity. As a result, reactions can be assembled at room temperature without non-specific amplification — improving bench ergonomics and making large-batch reaction setup viable — while multiplex specificity improves dramatically as mispriming risk among multiple primer pairs is suppressed, and GC-rich, low-copy, and fast PCR workflows all benefit from the suppressed non-specific amplification.
Application Profile (per Hot Start Brochure Section)
Per the Accuris Life Science Reagents Catalog, Hot Start Taq applications include colony PCR (direct PCR from a bacterial colony for clone screening), fast PCR (short-amplicon PCR with reduced cycle times), GC-rich DNA amplification (GC-rich templates with secondary-structure-driven mispriming risk), low-copy PCR (targets present at very low copy), and multiplex PCR (multi-primer-pair reactions where mispriming risk multiplies).
For routine PCR with well-optimized protocols and on-ice reaction assembly, the standard PR1000-1000 Taq is the cost-effective pick.
Reagent Composition
Per the Accuris Life Science Reagents Catalog, each PR1000-HS-1000 kit includes:
- 1 tube of Hot Start Taq DNA Polymerase at 5 U/µL (200 µL = 1,000 units)
- 4 tubes of 5X buffer containing PCR enhancers and 15 mM MgCl₂
The 5X buffer brings reactions to 1X with 3 mM MgCl₂ at standard dilution. dNTPs are not included — order separately from the Accuris dNTP catalog or supply from existing inventory.
Standalone Enzyme vs Master Mix
For Hot Start workflows where the convenience of pre-formulated 2X master mix is preferred over standalone enzyme + buffer + separate dNTPs, see the PR1001-HS family (Hot Start Taq 2X Master Mix) — PR1001-HS-200 / PR1001-HS-1000 ship 200 / 1,000 reactions of pre-formulated 2X master mix.
| Format | When to pick |
| PR1000-HS-1000 (this product) — standalone enzyme | Custom-formulation workflows, bulk-pack economics, dNTP independence, specialty buffer formulations |
| PR1001-HS-1000 — Hot Start Taq 2X Master Mix | Pre-formulated convenience, faster reaction setup, standardized buffer + dNTP across reactions |
Ordering & Configuration
dNTP procurement. dNTPs sold separately; plan to include both PR1000-HS-1000 and a dNTP mix on the order.
Storage & handling. Per Accuris kit instructions — typical Taq polymerase storage at −20 °C with limited freeze-thaw cycle tolerance.
Workflow fit verification. Confirm at order that the workflow benefits from Hot Start (multiplex / GC-rich / low-copy / room-temperature setup) — for routine PCR with on-ice assembly, the standard PR1000 is the cost-effective pick.
Returns. Per the universe price-list category header: reagent products including PCR enzymes are non-returnable due to perishable nature.
Confirm at quote: workflow specialty fit, pack-size match (500 vs 1,000 vs 6,000 units), standalone enzyme vs master-mix preference, dNTP procurement, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
Accuris PR1000-HS Hot Start Taq family:
- PR1000-HS-S — Sample size
- PR1000-HS-500 — 500 units
- PR1000-HS-1000 (this product) — 1,000 units (workhorse mid-throughput)
- PR1000-HS-6000 — 6,000 units (bulk-pack economics)
For master-mix convenience, see the PR1001-HS Hot Start 2X Master Mix family.
