The PR1001-HF-S is the trial / sample-size SKU of the Accuris High Fidelity Master Mix family, 5-reaction pack for first-use evaluation of the HF master mix format before committing to the larger production-scale packs (PR1001-HF-200 at $189 / PR1001-HF-500 at $420). At $7.65 list price, the sample SKU enables labs to validate the HF master mix performance on their specific PCR target before scaling procurement.
Key Specifications
| SKU | PR1001-HF-S |
| Type | Sample / trial pack / Accuris High Fidelity Master Mix |
| Pack quantity | 5 reactions |
| PCR mode | High Fidelity (HF): single-tube 2X master mix format |
| Format | Master mix (polymerase + buffer + dNTPs + MgCl₂ in single tube) |
| Sister SKUs | PR1001-HF-200 ($189), PR1001-HF-500 ($420), PR1001-HFHS-S (HF+Hot Start sample) |
| List price | $7.65 |
Sample Pack Family: All at $7.65
The PR1001 master mix family has two parallel sample SKUs at $7.65:
| Sample SKU | Master mix chemistry | Production-scale follow-on |
| PR1001-HF-S (this product) | High Fidelity master mix, 5 rxn | PR1001-HF-200 ($189) / PR1001-HF-500 ($420) |
| PR1001-HFHS-S | High Fidelity + Hot Start master mix, 5 rxn | PR1001-HFHS-200 ($186) / PR1001-HFHS-500 ($420) |
Procurement strategy. Labs comparing HF vs HFHS master mix chemistries can order both samples ($15.30 total) for a side-by-side evaluation on the lab's specific PCR target. It supports informed bulk-procurement decisions across the HF master mix family.
Plus the PR1000 family standalone polymerase samples (PR1000-S / PR1000-HS-S / PR1000-HF-S, all $7.65 each), full chemistry comparison for $46 across all 6 sample SKUs.
Why a Master Mix Sample vs Standalone Polymerase Sample
The Accuris HF chemistry is available in both standalone polymerase (PR1000-HF-S, 20 units) and master mix (PR1001-HF-S, 5 reactions) sample formats. The two samples evaluate different procurement options:
| Feature | PR1000-HF-S standalone sample | PR1001-HF-S master mix sample (this product) |
| Reaction setup | Multi-tube setup (polymerase + buffer + dNTPs etc.) | Single-tube setup (master mix + template / primers / water) |
| Trial scope | Validates HF chemistry + multi-tube setup workflow | Validates HF chemistry + single-tube convenience workflow |
| Production follow-on | PR1000-HF-200 / -1000 standalone polymerase | PR1001-HF-200 / -500 master mix |
Pick the PR1001-HF-S master mix sample when: the lab is evaluating the single-tube master mix convenience workflow for downstream procurement. Pick the PR1000-HF-S standalone sample when evaluating multi-tube cost-optimization workflow.
Per Brochure HF Applications
Per Accuris Life Science Reagents Catalog, HF Master Mix applications include:
- Blunt-end / TA cloning. HF polymerase produces blunt-end PCR products
- Colony PCR. When colony screening feeds into downstream sequencing-grade workflow
- Fast PCR. Short-amplicon HF amplification with reduced cycle times
- GC- and AT-rich DNA amplification. HF chemistry handles extreme-composition templates
- Low Copy PCR. Sensitive PCR from low-copy template
Application Profile
HF master mix first-time evaluation. Labs evaluating the PR1001 HF master mix format for the first time use the sample to validate fit before bulk procurement.
Master mix vs standalone comparison. Labs comparing master mix convenience vs standalone cost-optimization use both PR1001-HF-S and PR1000-HF-S samples for the comparison.
HF vs HFHS chemistry comparison. Labs comparing HF-only vs HF+Hot Start master mix chemistries use both samples for side-by-side validation.
Sales / training samples. Sales reps and training programs use the sample for hands-on customer demonstrations.
Educational laboratory PCR exercises. Molecular-biology coursework introducing HF chemistry on a per-class budget.
Ordering & Configuration
Single-use sample SKU. The 5-reaction pack supports a small validation run; not intended for routine production use.
Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; operator supplies template, primers, and water.
Confirm at quote: HF master mix evaluation intent (vs PR1001-HF-200 / -500 production-pack ordering, or step-up to PR1001-HFHS for hot-start chemistry), and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris PR1001 master mix sample SKUs (both $7.65):
- PR1001-HF-S (this product): HF Master Mix sample, 5 reactions
- PR1001-HFHS-S: HF + Hot Start Master Mix sample, 5 reactions
Pick the PR1001-HF-S sample for HF master mix evaluation; pair with PR1001-HFHS-S for HF vs HFHS chemistry comparison.
