The PR1001-HF-S is the trial / sample-size SKU of the Accuris High Fidelity Master Mix family — 5-reaction pack for first-use evaluation of the HF master mix format before committing to the larger production-scale packs (PR1001-HF-200 at $189 / PR1001-HF-500 at $420). At $7.65 list price, the sample SKU enables labs to validate the HF master mix performance on their specific PCR target before scaling procurement.
Key Specifications
| SKU | PR1001-HF-S |
| Type | Sample / trial pack — Accuris High Fidelity Master Mix |
| Pack quantity | 5 reactions |
| PCR mode | High Fidelity (HF) — single-tube 2X master mix format |
| Format | Master mix — polymerase + buffer + dNTPs + MgCl₂ in single tube |
| Sister SKUs | PR1001-HF-200 ($189), PR1001-HF-500 ($420), PR1001-HFHS-S (HF+Hot Start sample) |
| List price | $7.65 |
Sample Pack Family — All at $7.65
The PR1001 master mix family has two parallel sample SKUs at $7.65:
| Sample SKU | Master mix chemistry | Production-scale follow-on |
| PR1001-HF-S (this product) | High Fidelity master mix, 5 rxn | PR1001-HF-200 ($189) / PR1001-HF-500 ($420) |
| PR1001-HFHS-S | High Fidelity + Hot Start master mix, 5 rxn | PR1001-HFHS-200 ($186) / PR1001-HFHS-500 ($420) |
Procurement strategy. Labs comparing HF vs HFHS master mix chemistries can order both samples ($15.30 total) for a side-by-side evaluation on the lab's specific PCR target — supports informed bulk-procurement decisions across the HF master mix family.
Plus the PR1000 family standalone polymerase samples (PR1000-S / PR1000-HS-S / PR1000-HF-S, all $7.65 each) — full chemistry comparison for $46 across all 6 sample SKUs.
Why a Master Mix Sample vs Standalone Polymerase Sample
The Accuris HF chemistry is available in both standalone polymerase (PR1000-HF-S, 20 units) and master mix (PR1001-HF-S, 5 reactions) sample formats. The two samples evaluate different procurement options:
| Feature | PR1000-HF-S standalone sample | PR1001-HF-S master mix sample (this product) |
| Reaction setup | Multi-tube setup (polymerase + buffer + dNTPs etc.) | Single-tube setup (master mix + template / primers / water) |
| Trial scope | Validates HF chemistry + multi-tube setup workflow | Validates HF chemistry + single-tube convenience workflow |
| Production follow-on | PR1000-HF-200 / -1000 standalone polymerase | PR1001-HF-200 / -500 master mix |
Pick the PR1001-HF-S master mix sample when: the lab is evaluating the single-tube master mix convenience workflow for downstream procurement. Pick the PR1000-HF-S standalone sample when evaluating multi-tube cost-optimization workflow.
Per Brochure HF Applications
Per Accuris Life Science Reagents Catalog, HF Master Mix applications include:
- Blunt-end / TA cloning — HF polymerase produces blunt-end PCR products
- Colony PCR — when colony screening feeds into downstream sequencing-grade workflow
- Fast PCR — short-amplicon HF amplification with reduced cycle times
- GC- and AT-rich DNA amplification — HF chemistry handles extreme-composition templates
- Low Copy PCR — sensitive PCR from low-copy template
Application Profile
HF master mix first-time evaluation — labs evaluating the PR1001 HF master mix format for the first time use the sample to validate fit before bulk procurement.
Master mix vs standalone comparison — labs comparing master mix convenience vs standalone cost-optimization use both PR1001-HF-S and PR1000-HF-S samples for the comparison.
HF vs HFHS chemistry comparison — labs comparing HF-only vs HF+Hot Start master mix chemistries use both samples for side-by-side validation.
Sales / training samples — sales reps and training programs use the sample for hands-on customer demonstrations.
Educational laboratory PCR exercises — molecular-biology coursework introducing HF chemistry on a per-class budget.
Ordering & Configuration
Single-use sample SKU. The 5-reaction pack supports a small validation run; not intended for routine production use.
Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; operator supplies template, primers, and water.
Confirm at quote: HF master mix evaluation intent (vs PR1001-HF-200 / -500 production-pack ordering, or step-up to PR1001-HFHS for hot-start chemistry), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1001 master mix sample SKUs (both $7.65):
- PR1001-HF-S (this product) — HF Master Mix sample, 5 reactions
- PR1001-HFHS-S — HF + Hot Start Master Mix sample, 5 reactions
Pick the PR1001-HF-S sample for HF master mix evaluation; pair with PR1001-HFHS-S for HF vs HFHS chemistry comparison.
