Accuris High Fidelity Master Mix, 200 Reactions

The PR1001-HF-200 is the single-tube 2X High Fidelity master mix — sold as 200-reaction pack (2 × 1.25 mL tubes) per the Accuris Life Science Reagents Catalog. The HF master mix integrates the proofreading high-fidelity polymerase + buffer + dNTPs + MgCl₂ into a single tube, supporting the blunt-end PCR product workflows (TA cloning, blunt-end ligation) where proofreading polymerase produces blunt-end products vs standard Taq's 3' A-overhang.

Pack-format note: The HF master mix ships in 2 × 1.25 mL tubes for 200 reactions — different from the standard Taq 2X master mix (PR1001-200) which ships in 4 × 1.25 mL tubes for the same 200 reactions. The HF master mix runs at higher per-reaction volume (12.5 µL of 2X mix per reaction vs the standard Taq's 6.25 µL — confirm exact recommended per-reaction volume in the product insert).

Key Specifications

Per Brochure HF Master Mix Applications

Per Accuris Life Science Reagents Catalog, High Fidelity Master Mix applications include:

• Blunt-end / TA cloning — HF polymerase produces blunt-end PCR products that can be either ligated into blunt-end vectors directly, or treated for TA cloning workflows
• Colony PCR — when colony screening feeds into downstream sequencing-grade workflow
• Fast PCR — short-amplicon HF amplification with reduced cycle times
• GC- and AT-rich DNA amplification — HF chemistry handles extreme-composition templates better than standard Taq
• Low Copy PCR — sensitive PCR from low-copy template benefits from HF's reduced error accumulation across cycles

HF Master Mix vs HF Standalone Polymerase

The Accuris HF chemistry is available in both formats:

Same chemistry, different workflow profile. Pick the master mix for setup convenience; pick the standalone for per-reaction cost optimization at scale.

HF vs HF + Hot Start Master Mix Selection

The PR1001 HF master mix family includes two related master mix formats:

Pick the HF-only master mix when: the workflow benefits from HF accuracy (cloning, sequencing prep, mutagenesis) but the lab does not require hot-start chemistry's pre-activation suppression. Pick HFHS for workflows needing both.

Application Profile

Blunt-end PCR product cloning — the workhorse HF application. Blunt-end ligation workflows benefit from HF polymerase's blunt-end product chemistry.

Sequencing-prep PCR — fragment-extraction prep for Sanger or NGS library construction benefits from HF accuracy.

Site-directed mutagenesis — workflows where preserving template sequence accuracy matters benefit from HF chemistry's low error rate.

GC- and AT-rich amplification — extreme-composition templates benefit from HF chemistry over standard Taq.

Low-copy PCR — sensitive PCR from low template benefits from HF's reduced cumulative error.

Microarray template prep — workflows where sequence accuracy matters for downstream microarray analysis.

Multi-operator HF workflows — facilities running HF on shared benches benefit from the master-mix format's reduced setup-variability across operators.

Ordering & Configuration

Per-reaction workflow consumable. Each PCR reaction uses 12.5 µL of the 2X HF master mix (confirm exact volume in product insert). A 1.25 mL tube supports 100 reactions at 12.5 µL each; the 200-reaction pack is 2 × 1.25 mL tubes.

Storage. HF reagent storage applies — confirm storage protocol at order.

Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; operator supplies template, primers, and water (or PCR-grade buffer).

Confirm at quote: workflow PCR throughput (drives 200-rxn vs 500-rxn pack-size selection), HF vs HFHS decision (does the workflow need hot-start control), evaluation interest (PR1001-HF-S sample at $7.65 for first-use validation), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR1001 High Fidelity master mix family:

- PR1001-HF-S — Sample, 5 reactions ($7.65) — first-use evaluation

- PR1001-HF-200 (this product) — High Fidelity Master Mix, 200 reactions ($189)

- PR1001-HF-500 — High Fidelity Master Mix, 500 reactions ($420)

- PR1001-HFHS-200 — HF + Hot Start combined, 200 reactions ($186)

- PR1001-HFHS-500 — HF + Hot Start, 500 reactions ($420)

Pick the HF-only master mix for cloning / sequencing-prep / mutagenesis workflows; step to HFHS when the workflow also requires hot-start control.