The PR1001-HFHS-S is the trial / sample-size SKU of the Accuris High Fidelity + Hot Start combined master mix family, 5-reaction pack for first-use evaluation of the HFHS combined chemistry before committing to the larger production-scale packs (PR1001-HFHS-200 at $186 / PR1001-HFHS-500 at $420). At $7.65 list price, the sample SKU enables labs to validate the combined HF + Hot Start chemistry on their specific PCR target, particularly important for the multiplex HF / GC-rich HF / low-copy HF workflows where the combined chemistry is the right pick.
Key Specifications
| SKU | PR1001-HFHS-S |
| Type | Sample / trial pack (Accuris HF + Hot Start combined Master Mix) |
| Pack quantity | 5 reactions |
| PCR mode | High Fidelity + Hot Start (combined chemistry) |
| Format | Master mix (combined-chemistry polymerase + buffer + dNTPs + MgCl₂ in single tube) |
| Sister sample SKU | PR1001-HF-S (HF-only sample, 5 rxn, $7.65) |
| Production-scale sister SKUs | PR1001-HFHS-200 ($186), PR1001-HFHS-500 ($420) |
| List price | $7.65 |
HF vs HFHS Sample Pair: Direct Chemistry Comparison
The two PR1001 master mix sample SKUs at $7.65 each enable a direct HF vs HFHS chemistry comparison on the lab's specific PCR target:
| Sample SKU | Chemistry | When the workflow needs |
| PR1001-HF-S | HF only | HF accuracy without hot-start activation control |
| PR1001-HFHS-S (this product) | HF + Hot Start combined | HF accuracy AND hot-start activation control (multiplex, GC-rich, low-copy, primer-dimer-prone) |
Procurement strategy. Labs running multiplex HF cloning / GC-rich HF / low-copy HF workflows benefit from ordering both samples ($15.30 total) for a side-by-side validation. Confirm whether the workflow actually needs the hot-start premium of HFHS or if HF-only delivers sufficient performance.
Plus the standalone polymerase samples (PR1000-HF-S / PR1000-HS-S / PR1000-S, all $7.65) for a complete HF + Hot Start chemistry-evaluation workflow at $46 across all 6 sample SKUs.
Per Brochure HFHS Applications
Per the Accuris Life Science Reagents Catalog, the HFHS Master Mix combines applications from both chemistries. From HF chemistry it covers blunt-end / TA cloning, sequencing-prep PCR, site-directed mutagenesis, and GC- and AT-rich amplification; from Hot Start chemistry it covers multiplex PCR, low-copy PCR, and primer-dimer-prone setups.
The combined-chemistry sweet spot is where both constraints apply at once:
- Multiplex HF cloning
- GC-rich HF amplification with downstream sequencing
- Low-copy HF amplification
Application Profile
HFHS chemistry first-time evaluation. Labs evaluating the combined HF + Hot Start master mix for the first time use the sample to validate fit before bulk procurement.
HF vs HFHS chemistry comparison. Labs deciding between HF-only and HFHS run both samples (PR1001-HF-S + PR1001-HFHS-S) for direct workflow comparison.
Multiplex HF workflow validation. Labs developing multiplex HF protocols use the sample to validate that HFHS handles the multiplex specificity needs.
Sales / training samples. Sales reps and training programs use the sample for hands-on customer demonstrations.
Educational laboratory PCR exercises. Molecular-biology coursework introducing the concept of combined-chemistry PCR reagents.
Ordering & Configuration
Single-use sample SKU. The 5-reaction pack supports a small validation run; not intended for routine production use.
Pair with template / primers / water. The master mix includes the polymerase + buffer + dNTPs + MgCl₂; operator supplies template, primers, and water.
Confirm at quote: HFHS chemistry evaluation intent (vs PR1001-HFHS-200 / -500 production-pack ordering), comparison to HF-only (PR1001-HF-S sample for parallel validation), and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris PR1001 master mix sample SKUs (both $7.65):
- PR1001-HF-S: HF Master Mix sample, 5 reactions
- PR1001-HFHS-S (this product): HF + Hot Start Master Mix sample, 5 reactions
Pick the PR1001-HFHS-S sample for combined-chemistry evaluation; pair with PR1001-HF-S for HF vs HFHS direct chemistry comparison.
