The PR1001-HFHS-500 is the larger 500-reaction pack of the Accuris High Fidelity + Hot Start combined master mix — sold as 5 × 1.25 mL tubes per the brochure. Same HFHS chemistry as the PR1001-HFHS-200 sister (combined HF proofreading + antibody-mediated hot-start activation), engineered for higher-throughput labs that consume the HFHS master mix at production scale.
For complete chemistry rationale, application profile, and HFHS vs HF-only vs Hot Start-only decision, see the PR1001-HFHS-200 parent page.
Key Specifications
| SKU | PR1001-HFHS-500 |
| Type | Single-tube 2X HF + Hot Start combined master mix (larger pack) |
| Pack quantity | 500 reactions (5 × 1.25 mL tubes) |
| PCR mode | High Fidelity + Hot Start (combined chemistry) |
| Per-reaction cost | $0.84 per reaction (vs $0.93 per reaction at the 200-rxn pack) |
| Sister SKUs | PR1001-HFHS-200 (200 reactions, $186), PR1001-HFHS-S (sample 5 rxn, $7.65) |
| List price | $420 |
Per-Reaction Cost Economics
The PR1001-HFHS family pack-size economics:
| Pack | Reactions | List price | Per-reaction cost |
| PR1001-HFHS-S | 5 | $7.65 | $1.53 |
| PR1001-HFHS-200 | 200 | $186 | $0.93 |
| PR1001-HFHS-500 (this product) | 500 | $420 | $0.84 |
Pick the 500-reaction pack when:
- Workflow throughput consumes 200+ HFHS reactions per quarter (the 500-rxn pack provides ~10 % per-reaction savings vs the 200-rxn pack)
- Production-scale workflows where the larger pack reduces reorder frequency
- Multi-chassis facilities with consolidated HFHS workflow across multiple PCR thermocyclers
Pick the 200-rxn pack when: workflow throughput is < 200 rxn per quarter and the larger pack would expire before consumption.
Workflow Throughput Sizing
| Workflow scenario | Per-week HFHS rxn count | Pack-size recommendation |
| Light use (5–10 HFHS rxn / week) | 250–500 / year | Sample + 200-rxn pack (validates + 1 production cycle / year) |
| Routine HF cloning workflow (10–25 HFHS rxn / week) | 500–1,250 / year | 200-rxn pack (~5–6 reorders / year) OR 500-rxn pack (~2–3 reorders / year) |
| Production-scale HF cloning core (50+ HFHS rxn / week) | 2,500+ / year | 500-rxn pack (5+ reorders / year, per-rxn cost savings matter at this throughput) |
Application Profile
See the PR1001-HFHS-200 parent page for the full application profile (multiplex HF cloning, GC-rich HF amplification, low-copy HF, sequencing-prep multiplex, method-development PCR optimization, pharma / biotech screening with downstream sequencing).
The 500-reaction pack is the preferred procurement for production-scale HFHS workflows where the per-reaction cost saving over the 200-rxn pack accumulates meaningfully across the annual workflow volume.
Ordering & Configuration
Per-reaction workflow consumable. Same per-reaction usage as the 200-rxn pack — 12.5 µL of 2X master mix per PCR reaction. The 500-rxn pack is 5 × 1.25 mL tubes.
Storage. Same HFHS reagent storage as the 200-rxn pack — confirm storage protocol at order.
Confirm at quote: workflow throughput sizing (justifies 500-rxn pack vs 200-rxn pack), HFHS chemistry need confirmation, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1001-HFHS combined HF + Hot Start master mix family:
- PR1001-HFHS-S — Sample, 5 reactions ($7.65, $1.53/rxn)
- PR1001-HFHS-200 — 200 reactions ($186, $0.93/rxn)
- PR1001-HFHS-500 (this product) — 500 reactions ($420, $0.84/rxn)
Pick the 500-reaction pack for production-scale HFHS workflows where the per-reaction cost saving matters; step down to the 200-rxn pack for moderate-throughput workflows or the sample for first-use evaluation.
