The PR1000-HF-S is the trial / sample-size SKU of the Accuris High Fidelity DNA Polymerase family, 20 units of HF Polymerase at 2 U/µL (10 µL total) for first-use evaluation of the HF chemistry before committing to the larger production-scale packs (PR1000-HF-200 at $175 / PR1000-HF-1000 at higher list). At $7.65 list price, the sample SKU enables labs to validate the HF chemistry on their specific PCR target before scaling procurement.
Key Specifications
| SKU | PR1000-HF-S |
| Type | Sample / trial pack / Accuris High Fidelity DNA Polymerase |
| Quantity | 20 units (10 µL at 2 U/µL) |
| Polymerase concentration | 2 U/µL (HF standard) |
| Included buffer | 5X buffer with PCR enhancers, 5 mM dNTPs, 15 mM MgCl₂ (HF buffer INCLUDES dNTPs; different from standard Taq buffer) |
| Trial reaction count | ~10 reactions at 2 U/reaction (typical HF reaction setup) |
| Sister SKUs (production scale) | PR1000-HF-200 (200 units, $175), PR1000-HF-1000 (1000 units) |
| PCR mode | High Fidelity (proofreading polymerase with low error rate) |
| List price | $7.65 |
Why a Sample Pack: Trial Before Bulk Procurement
PCR enzyme procurement at production scale (200 / 1,000 / 6,000 units) represents a meaningful budget commitment per kit. The PR1000-HF-S sample pack at $7.65 enables labs to test the HF chemistry on their specific PCR targets before scaling:
| Validation step | Sample-pack support |
| Protocol-fit verification | ~10 reactions support a 2–4 condition optimization run (annealing temp, MgCl₂ concentration, template input) |
| Target-specificity check | Test HF performance on the lab's specific amplicon (GC-content, length, secondary structure considerations) |
| Buffer-compatibility check | Confirm the HF buffer + dNTP formulation works with the lab's existing template prep and downstream workflow |
| Comparison vs standard Taq | Direct comparison to PR1000 standard Taq for fidelity-vs-throughput trade-off decision |
Workflow benefit. The $7.65 sample evaluation cost is < 5 % of the PR1000-HF-200 production-pack cost, meaningful risk mitigation before a bulk procurement decision.
High Fidelity vs Standard Taq Decision
| Feature | PR1000 Standard Taq | PR1000-HF Family (this sample) |
| Polymerase concentration | 5 U/µL | 2 U/µL |
| Proofreading | No | Yes (3'→5' exonuclease activity) |
| Error rate | ~1×10⁻⁴ (standard Taq) | ~1×10⁻⁶ (HF): 100× lower |
| Buffer formulation | dNTPs sold separately | dNTPs INCLUDED in 5X buffer |
| Best fit | Routine endpoint PCR, colony PCR, screening | Cloning, sequencing prep, mutagenesis, microarray prep (workflows where sequence accuracy matters) |
Pick HF over standard Taq when: the PCR product goes into downstream sequencing, cloning, mutagenesis, or other workflows where sequence accuracy matters. The 100× error-rate improvement is critical for these applications.
Per Brochure HF Applications
Per Accuris Life Science Reagents Catalog, High Fidelity Polymerase applications include:
- Blunt-end cloning. HF polymerase produces blunt-end PCR products (vs standard Taq's 3' A-overhang); blunt-end is required for some cloning workflows
- Colony PCR. When colony screening feeds into downstream sequencing-grade workflow
- Fast PCR. Short-amplicon HF amplification with reduced cycle times
- GC- and AT-rich DNA amplification. HF chemistry handles extreme-composition templates better than standard Taq
- Low Copy PCR. Sensitive PCR from low-copy template benefits from HF's reduced error accumulation across cycles
Application Profile
HF chemistry first-time evaluation. Labs evaluating Accuris HF Polymerase for the first time use the sample pack to validate fit before bulk procurement.
New PCR-target validation. Labs adding HF chemistry to a new amplicon validate the protocol on the sample pack before committing.
Sales / training samples. Sales reps and training programs use the sample pack for hands-on customer demonstrations.
Educational laboratory PCR exercises. Molecular-biology coursework introducing HF polymerase chemistry on a per-class budget.
Method-development workflows. Protocol-development workflows where HF performance is one of several enzyme options being compared.
Ordering & Configuration
Single-use sample SKU. The 20-unit pack supports a single evaluation run; not intended for routine production use.
Pair with dNTPs and template / primer / reagents. Unlike standard Taq, the HF buffer includes dNTPs. The operator only needs to supply template, primers, and downstream workflow reagents.
Confirm at quote: HF chemistry evaluation intent (vs production-pack ordering), production-pack interest (PR1000-HF-200 follow-on), and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Accuris PR1000-HF High Fidelity Polymerase family:
- PR1000-HF-S (this product): Sample, 20 units ($7.65), first-use evaluation
- PR1000-HF-200, 200 units ($175), small lab production pack
- PR1000-HF-1000, 1000 units, mid-throughput production pack
Pick the sample for HF chemistry evaluation; step to the 200-unit or 1000-unit pack for production workflows.
