The PR1000-HF-200 is the 200-unit pack of the Accuris High Fidelity DNA Polymerase family — a high-fidelity polymerase with low error rate for cloning, sequencing template prep, mutagenesis, and microarray applications where polymerase fidelity matters more than the fastest cycling speed. Polymerase concentration is 2 U/µL (lower than the 5 U/µL standard Taq, typical for high-fidelity polymerases). Each kit ships with 1 tube of High Fidelity Polymerase (100 µL at 2 U/µL = 200 units) + 3 tubes of 5X buffer that — uniquely in the PR1000 family — already includes 5 mM dNTPs plus PCR enhancers and 15 mM MgCl₂.
Key Specifications
| SKU | PR1000-HF-200 |
| Type | High Fidelity DNA Polymerase — low-error-rate enzyme |
| Quantity | 200 units (100 µL at 2 U/µL) |
| Polymerase concentration | 2 U/µL (note: half of standard Taq's 5 U/µL — typical HF concentration) |
| Included buffer | 3 tubes of 5X buffer with PCR enhancers, 5 mM dNTPs, and 15 mM MgCl₂ |
| dNTPs | INCLUDED in the buffer (unique among PR1000 family) — no separate dNTP procurement |
| PCR mode | Endpoint PCR — high-fidelity for cloning / sequencing / mutagenesis |
| PCR product format | Blunt-end (high-fidelity polymerases typically lack the terminal-A addition activity of standard Taq) |
| Larger pack sister | PR1000-HF-1000 (1,000 units) |
| Standard-Taq sister family | PR1000 (5 U/µL, no dNTP-in-buffer, lower fidelity, lower cost) |
| Hot-start sister family | PR1000-HS (5 U/µL Hot Start, no dNTP-in-buffer) |
| HF Master Mix sister family | PR1001-HF (pre-formulated HF 2X Master Mix); PR1001-HFHS (HF + Hot Start combined) |
| Returns | Non-returnable — perishable reagent per Accuris reagent-return policy |
Why "High Fidelity" Matters
Standard Taq DNA Polymerase has a measurable error rate during DNA synthesis — base-substitution errors and small insertion / deletion errors that accumulate across cycles. For routine PCR (analytical sizing, genotyping, amplicon detection), the error rate is irrelevant because the bulk PCR product faithfully represents the template at the population level even if individual molecules carry occasional errors.
For workflows where individual PCR product molecules will be cloned, sequenced, or used as functional templates, the polymerase error rate matters because errors accumulate into the cloned, sequenced, or functional output. High-fidelity polymerases reduce the error rate by 10–100× vs standard Taq through 3'→5' exonuclease proofreading activity that detects and corrects misincorporation errors during synthesis.
| Workflow | Error rate sensitivity | Right polymerase |
| Genotyping, analytical PCR, amplicon detection | Low (population-level readout) | Standard Taq (PR1000) |
| Cloning of PCR products | High (cloned plasmid carries the errors forward) | High Fidelity (PR1000-HF) |
| Sequencing template prep | High (errors look like real variants) | High Fidelity (PR1000-HF) |
| Site-directed mutagenesis | High (must preserve all positions except the targeted site) | High Fidelity (PR1000-HF) |
| Microarray template prep | High (multi-position fidelity matters) | High Fidelity (PR1000-HF) |
Blunt-End PCR Products — Cloning Implication
Per the Accuris brochure, the High Fidelity polymerase produces blunt-end PCR products (suitable for blunt-end cloning vectors). Standard Taq adds a 3'-terminal A overhang that pairs with T-overhang TA-cloning vectors; high-fidelity polymerases lack this terminal-A activity because the proofreading exonuclease removes terminal mismatches.
Workflow implication:
- Blunt-end cloning vectors — direct ligation of HF PCR products
- TA-cloning vectors — require post-PCR A-tailing step (separate Taq or A-tailing reagent) before TA ligation
- Restriction-site cloning — restriction-digest the PCR product first to generate sticky ends; HF polymerase choice doesn't affect this workflow
Buffer Formulation — dNTP Inclusion is Unique
The HF buffer formulation already includes 5 mM dNTPs (1 mM in 1X final reaction) — distinct from the standard PR1000 and PR1000-HS buffers which require dNTPs to be added separately. This simplifies reaction setup (combine HF polymerase + 5X buffer + primers + template with no separate dNTP pipetting step), standardizes the dNTP concentration at the 1 mM final appropriate for high-fidelity PCR, and simplifies procurement (no separate dNTP purchase required, unlike PR1000 / PR1000-HS where dNTPs are sold separately).
For workflows that need different dNTP concentrations or specialty dNTP formulations (modified bases, hot-start dNTPs, fluorescent-label dNTPs), supplemental dNTPs are needed alongside the HF kit.
Application Profile (per Brochure)
Per the Accuris Life Science Reagents Catalog, High Fidelity DNA Polymerase applications include blunt-end cloning (direct ligation of HF PCR products into blunt-end vectors), colony PCR where the downstream use of the product requires fidelity, fast PCR for short amplicons with reduced cycle times, GC- & AT-rich DNA amplification on challenging-composition templates where the proofreading activity helps recover from mispriming, and low-copy PCR for low-input templates where every accurate amplification matters.
The brochure also highlights HF polymerase as "Ideal for cloning (blunt end), mutagenesis, & microarray applications."
PR1000-HF Pack-Size Family
| SKU | Units | Use case |
| PR1000-HF-200 (this product) | 200 | Smaller pack — pilot work, occasional HF use, mid-cohort cloning workflows |
| PR1000-HF-1000 | 1,000 | Mid-throughput HF workflows — production cloning, sequencing-template prep at volume |
Standalone Enzyme vs HF Master Mix
For HF workflows where the convenience of pre-formulated 2X master mix is preferred, see the PR1001-HF family (HF 2X Master Mix) — PR1001-HF-200 / PR1001-HF-500 ship 200 / 500 reactions of pre-formulated HF 2X master mix. For HF + Hot Start combined chemistry (challenging templates that benefit from both proofreading and hot-start specificity), see PR1001-HFHS (HF Hot Start 2X Master Mix) at PR1001-HFHS-200 / PR1001-HFHS-500.
| Format | When to pick |
| PR1000-HF-200 (this product) — standalone enzyme | Custom-buffer workflows, supplemental MgCl₂ workflows, cost-control on enzyme volume |
| PR1001-HF-200 / PR1001-HF-500 — HF 2X Master Mix | Pre-formulated convenience, faster setup |
| PR1001-HFHS-200 / PR1001-HFHS-500 — HF + Hot Start combined | Challenging templates needing both proofreading and hot-start specificity |
Ordering & Configuration
dNTP independence. Unlike PR1000 / PR1000-HS, dNTPs are pre-formulated in the HF buffer — no separate dNTP procurement needed for routine HF workflows.
Storage & handling. Per Accuris kit instructions — typical HF polymerase storage at −20 °C with limited freeze-thaw cycle tolerance.
Workflow fit verification. Confirm at order that the downstream workflow requires HF chemistry (cloning, sequencing, mutagenesis, microarray) — for routine analytical PCR, the standard PR1000 at lower cost is the right pick.
Cloning-vector compatibility verification. Blunt-end PCR products from HF polymerase work directly with blunt-end cloning vectors; TA-cloning workflows need a post-PCR A-tailing step. Confirm cloning-vector format at order.
Returns. Per the universe price-list category header: reagent products including PCR enzymes are non-returnable due to perishable nature.
Confirm at quote: downstream workflow (cloning / sequencing / mutagenesis / microarray fit), pack-size match (200 vs 1,000 units), standalone enzyme vs master-mix preference, cloning-vector format (blunt vs TA), shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
Accuris PR1000-HF High Fidelity Polymerase family:
- PR1000-HF-200 (this product) — 200 units (smaller pack)
- PR1000-HF-1000 — 1,000 units (mid-throughput)
- PR1001-HF-200 / PR1001-HF-500 — HF 2X Master Mix (pre-formulated convenience)
- PR1001-HFHS-200 / PR1001-HFHS-500 — HF + Hot Start combined master mix
Pick standalone HF enzyme for custom-buffer workflows; pick HF master mix for pre-formulated convenience; pick HF+HS for challenging templates needing both proofreading and hot-start specificity.
