Accuris 1 Hour Mammalian Genotyping Kit, 400 Reactions

The Accuris 1 Hour Mammalian Genotyping Kit is a complete DNA extraction + amplification workflow for mammalian-tissue genotyping. The PR1300-MG-400 is the 400-reaction pack for production-scale genotyping operations. Per the Accuris Life Science Reagents Catalog: *"DNA extraction & amplification in as little as 1 hour. Designed for use with tail snips, ear punches, blood, FFPE tissue sections, buccal swabs, hair, & solid animal tissues."* The kit collapses the traditional 2–4 hour mammalian DNA extraction + 1–2 hour PCR setup workflow into a single 1-hour workflow by integrating the lysis buffer + protease buffer + Hot Start Taq Red Dye Master Mix into a pre-configured 3-tube kit format.

Key Specifications

Why a 1-Hour Workflow vs Traditional Genotyping

Traditional mammalian genotyping workflow:

1. DNA extraction (1.5–3 hours): sample lysis, protease digestion, organic extraction or column purification, DNA precipitation
2. DNA quantification (15–30 min): A260 or fluorometric measurement
3. PCR setup (15–30 min): master mix preparation, template addition, plate / tube loading
4. Thermal cycling (1.5–2.5 hours): typical genotyping PCR program
5. Gel loading + electrophoresis (30–60 min): loading dye addition, gel preparation if needed, electrophoresis run
6. Total: 4–7 hours

1 Hour Mammalian Genotyping Kit workflow:

1. Lysis + Protease digestion (~15 min): combined Lysis Buffer + Protease Buffer step
2. Direct PCR setup (~5 min): direct addition of Hot Start Taq Red Dye Master Mix to the lysate (no DNA purification step)
3. Thermal cycling (~30–40 min): optimized rapid PCR program
4. Direct gel loading (~5 min). Red Dye allows direct gel load without separate loading-dye step
5. Electrophoresis (~10–15 min for short genotyping amplicons)
6. Total: ~1 hour

Workflow advantages. There is no DNA purification step (Hot Start Taq tolerates the lysate directly) and no DNA quantification step, since the protocol is optimized for the typical lysate template input range. The combined kit format (lysis + protease + master mix in matched-quantity tubes) simplifies inventory, and the Red Dye enables direct gel loading without a separate loading-dye addition step.

Per Brochure Mammalian Genotyping Applications

Per the Accuris Life Science Reagents Catalog, the PR1300 1 Hour Mammalian Genotyping Kit's primary application is DNA extraction and amplification from mammalian tissue, including blood (drop / tail-vein samples for mouse and rat colony genotyping), buccal swabs, and hair for non-invasive sampling.

The kit is compatible with tail snips (the primary mouse-colony genotyping sample type), ear punches (primary mouse / rat colony identification and genotyping), blood, FFPE tissue sections for combined histology and genotyping workflows, buccal swabs, hair, and solid animal tissues from biopsy or dissection.

PR1300 Pack-Size Family

Pick the PR1300-MG-400 pack when:

• The lab runs mouse / rat colony genotyping at production scale, animal-facility colony management with weekly genotyping batches
• Workflow throughput is 400+ genotyping reactions per quarter, the per-reaction cost saving over the 80-rxn pack accumulates meaningfully
• Multi-investigator shared animal-facility procurement consolidation

Application Profile

Mouse / rat transgenic colony genotyping. The workhorse application. Animal-facility colony management with regular genotyping for breeding-pair selection and offspring genotype tracking.

Genetic-modification screening. CRISPR / transgenic / knockout mouse / rat genotyping after gene-modification protocols.

Drug-discovery animal model genotyping. Pharma / biotech animal model facilities running genotype confirmation for in-vivo studies.

Veterinary diagnostics. Companion-animal genetic testing workflows.

Forensic mammalian sample identification. Wildlife / forensic genotyping workflows.

Educational laboratory mammalian genetics exercises. Undergraduate / graduate genetics coursework with hands-on mouse / rat genotyping demonstrations.

Production-scale animal-facility consolidated procurement. Large transgenic-mouse facilities running 400+ genotyping reactions per month benefit from the production-scale pack.

Ordering & Configuration

Sample-handling protocol. Per brochure, sample types include tail snips, ear punches, blood, FFPE, buccal swabs, hair, solid tissues. Confirm specific sample-prep protocol with the kit insert (typical: small tissue piece into Lysis Buffer + Protease Buffer step).

Storage. Genotyping kit storage applies. Confirm storage protocol at order. Lysis / Protease buffers may have different storage requirements than the Hot Start Taq Red Dye Master Mix.

Pair with primer pairs. The kit includes the lysis + protease + master mix; operator supplies the gene-specific genotyping primer pairs (typically genotype-specific WT vs mutant amplicon primers).

Confirm at quote: workflow throughput (justifies 400-rxn pack vs 80-rxn pack), animal-facility scale, workflow sample types (tail snip vs blood vs FFPE), and lead time.

Warranty: Per Accuris standard reagent terms. Confirm at quote.

Accuris PR1300 1 Hour Mammalian Genotyping Kit family:

- PR1300-MG-S: Sample

- PR1300-MG-80: 80 reactions

- PR1300-MG-400 (this product): 400 reactions ($646, $1.62/rxn)

Pick the 400-reaction pack for production-scale animal-facility genotyping; step down to 80-rxn or sample for moderate / evaluation workflows.