The Accuris 1 Hour Mammalian Genotyping Kit is a complete DNA extraction + amplification workflow for mammalian-tissue genotyping — the PR1300-MG-400 is the 400-reaction pack for production-scale genotyping operations. Per the Accuris Life Science Reagents Catalog: *"DNA extraction & amplification in as little as 1 hour. Designed for use with tail snips, ear punches, blood, FFPE tissue sections, buccal swabs, hair, & solid animal tissues."* The kit collapses the traditional 2–4 hour mammalian DNA extraction + 1–2 hour PCR setup workflow into a single 1-hour workflow by integrating the lysis buffer + protease buffer + Hot Start Taq Red Dye Master Mix into a pre-configured 3-tube kit format.
Key Specifications
| SKU | PR1300-MG-400 |
| Type | Complete mammalian genotyping kit (DNA extraction + amplification) |
| Pack quantity | 400 reactions (5 × the 80-reaction kit format) |
| Total workflow time | As little as 1 hour (DNA extraction + amplification combined) |
| Compatible sample types | Tail snips, ear punches, blood, FFPE tissue sections, buccal swabs, hair, solid animal tissues |
| Per 80-reaction kit format | 1 tube of 5X Lysis Buffer + 1 tube of 10X Protease Buffer + 2 tubes of Hot Start Taq 2X Red Dye Master Mix |
| Hot Start chemistry | Hot Start Taq Master Mix included → room-temperature reaction setup without non-specific amplification |
| Red Dye for direct gel loading | Included in the Hot Start Taq Red Dye Master Mix → no separate loading-dye addition step |
| Sister SKUs | PR1300-MG-80 (80 reactions), PR1300-MG-S (sample reactions) |
| Per-reaction cost | $1.62 per reaction |
| List price | $646 |
Why a 1-Hour Workflow vs Traditional Genotyping
Traditional mammalian genotyping workflow:
- DNA extraction (1.5–3 hours) — sample lysis, protease digestion, organic extraction or column purification, DNA precipitation
- DNA quantification (15–30 min) — A260 or fluorometric measurement
- PCR setup (15–30 min) — master mix preparation, template addition, plate / tube loading
- Thermal cycling (1.5–2.5 hours) — typical genotyping PCR program
- Gel loading + electrophoresis (30–60 min) — loading dye addition, gel preparation if needed, electrophoresis run
- Total: 4–7 hours
1 Hour Mammalian Genotyping Kit workflow:
- Lysis + Protease digestion (~15 min) — combined Lysis Buffer + Protease Buffer step
- Direct PCR setup (~5 min) — direct addition of Hot Start Taq Red Dye Master Mix to the lysate (no DNA purification step)
- Thermal cycling (~30–40 min) — optimized rapid PCR program
- Direct gel loading (~5 min) — Red Dye allows direct gel load without separate loading-dye step
- Electrophoresis (~10–15 min for short genotyping amplicons)
- Total: ~1 hour
Workflow advantages. There is no DNA purification step — Hot Start Taq tolerates the lysate directly — and no DNA quantification step, since the protocol is optimized for the typical lysate template input range. The combined kit format (lysis + protease + master mix in matched-quantity tubes) simplifies inventory, and the Red Dye enables direct gel loading without a separate loading-dye addition step.
Per Brochure Mammalian Genotyping Applications
Per the Accuris Life Science Reagents Catalog, the PR1300 1 Hour Mammalian Genotyping Kit's primary application is DNA extraction and amplification from mammalian tissue, including blood (drop / tail-vein samples for mouse and rat colony genotyping), buccal swabs, and hair for non-invasive sampling.
The kit is compatible with tail snips (the primary mouse-colony genotyping sample type), ear punches (primary mouse / rat colony identification and genotyping), blood, FFPE tissue sections for combined histology and genotyping workflows, buccal swabs, hair, and solid animal tissues from biopsy or dissection.
PR1300 Pack-Size Family
| SKU | Reactions | List price | Per-reaction cost |
| PR1300-MG-S | (sample) | (universe pricing) | (sample) |
| PR1300-MG-80 | 80 | (universe pricing) | (~$2.06/rxn estimate) |
| PR1300-MG-400 (this product) | 400 | $646 | $1.62/rxn |
Pick the PR1300-MG-400 pack when:
- The lab runs mouse / rat colony genotyping at production scale — animal-facility colony management with weekly genotyping batches
- Workflow throughput is 400+ genotyping reactions per quarter — the per-reaction cost saving over the 80-rxn pack accumulates meaningfully
- Multi-investigator shared animal-facility procurement consolidation
Application Profile
Mouse / rat transgenic colony genotyping — the workhorse application. Animal-facility colony management with regular genotyping for breeding-pair selection and offspring genotype tracking.
Genetic-modification screening — CRISPR / transgenic / knockout mouse / rat genotyping after gene-modification protocols.
Drug-discovery animal model genotyping — pharma / biotech animal model facilities running genotype confirmation for in-vivo studies.
Veterinary diagnostics — companion-animal genetic testing workflows.
Forensic mammalian sample identification — wildlife / forensic genotyping workflows.
Educational laboratory mammalian genetics exercises — undergraduate / graduate genetics coursework with hands-on mouse / rat genotyping demonstrations.
Production-scale animal-facility consolidated procurement — large transgenic-mouse facilities running 400+ genotyping reactions per month benefit from the production-scale pack.
Ordering & Configuration
Sample-handling protocol. Per brochure, sample types include tail snips, ear punches, blood, FFPE, buccal swabs, hair, solid tissues — confirm specific sample-prep protocol with the kit insert (typical: small tissue piece into Lysis Buffer + Protease Buffer step).
Storage. Genotyping kit storage applies — confirm storage protocol at order. Lysis / Protease buffers may have different storage requirements than the Hot Start Taq Red Dye Master Mix.
Pair with primer pairs. The kit includes the lysis + protease + master mix; operator supplies the gene-specific genotyping primer pairs (typically genotype-specific WT vs mutant amplicon primers).
Confirm at quote: workflow throughput (justifies 400-rxn pack vs 80-rxn pack), animal-facility scale, workflow sample types (tail snip vs blood vs FFPE), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris PR1300 1 Hour Mammalian Genotyping Kit family:
- PR1300-MG-S — Sample
- PR1300-MG-80 — 80 reactions
- PR1300-MG-400 (this product) — 400 reactions ($646, $1.62/rxn)
Pick the 400-reaction pack for production-scale animal-facility genotyping; step down to 80-rxn or sample for moderate / evaluation workflows.
