The Accuris PR6000-5K is the 5,000-reaction mid-tier pack of the SmartDye™ Terminator v3.1 Cycle Sequencing Kit — the institutional workhorse pack in the family. It is a cycle sequencing kit based on the Sanger method, containing fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer, and is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit — requiring no protocol adjustment, mobility-shift files, or recalibration. Sized for institutional Sanger sequencing operations running 5,000 reactions per pack-purchase cycle. Pricing scales linearly from the PR6000-1K (1,000 reactions at $7,800 = $7.80/rxn): the 5K pack at $39,000 = $7.80/rxn (no bulk discount until the PR6000-25K tier). The 5K pack's procurement value is operational (single procurement cycle covers 5,000 reactions, reduces reorder frequency, and enables single-lot consistency across workflow batches) rather than purely cost-driven.
Accuris's proprietary optimized dye chemistry delivers a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions. Reaction time is as short as 3 minutes, and the chemistry stays effective at minimal volumes (0.15µl dye in a 5µl total reaction). Applications span de novo sequencing, resequencing, and finishing, and it is compatible with PCR products, plasmids, BACs, and fosmids.
🚨 Safety flag: HIGH_PRICE_NO_CAPITAL_KW. $39,000 consumable pricing is well above typical thresholds — flagged for procurement and class-verification review.
Key Specifications
| SKU | PR6000-5K |
| Full product name | SmartDye™ Terminator v3.1 Cycle Sequencing Kit |
| Type | Sanger cycle sequencing kit — dye-terminator chemistry — 5,000 reactions (institutional workhorse) |
| Reactions per kit | 5,000 reactions |
| Kit contents | Dye Mix 2×20ml; 5X Buffer 12ml |
| Kit components | Fluorescently labelled dideoxynucleotide terminators, DNA polymerase, 5X optimized buffer |
| Commercial equivalence | Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 — no protocol adjustment, mobility-shift files, or recalibration |
| Reaction time | As short as 3 minutes |
| Minimal reaction volume | Effective at 0.15µl dye in a 5µl total reaction |
| Per-reaction cost | $7.80 (linear with PR6000-1K; no bulk discount until 25K tier) |
| List price | $39,000 |
| Safety flag | HIGH_PRICE_NO_CAPITAL_KW |
SmartDye Pack-Size Family
| SKU | Reactions | Price | Per-reaction |
| PR6000-1K | 1,000 | $7,800 | $7.80 |
| PR6000-5K (this product) | 5,000 | $39,000 | $7.80 |
| PR6000-25K | 25,000 | $185,000 | $7.40 (5% discount) |
The 5K pack is the right pick for institutional Sanger sequencing operations running 5,000+ reactions per quarter — sequencing service core facilities, large clinical-research labs, multi-cohort translational research workflows. For lower-throughput labs, the PR6000-1K is the right pick; for genome centers and high-volume sequencing services, the PR6000-25K delivers modest bulk discount.
Application Profile
Institutional Sanger sequencing operations — academic and commercial sequencing service core facilities running cycle sequencing at 5,000+ reactions per pack-purchase cycle.
Multi-cohort translational research workflows — clinical-research studies that require single-lot SmartDye consistency across multiple cohorts.
Mid-volume sequencing service contracts — service operations with committed sequencing volumes that justify upfront procurement of the 5K mid-tier bulk pack.
Ordering & Configuration
Confirm at order: sequencer platform compatibility and storage conditions (neither is stated on the manufacturer product page — confirm at order), plus expected workflow throughput and procurement-approval scope. Chemistry and kit composition are confirmed: fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer; the 5K pack ships as Dye Mix 2×20ml and 5X Buffer 12ml.
Storage & handling. Storage conditions are not stated on the manufacturer product page — confirm at order.
Confirm at quote: sequencer platform compatibility, expected workflow throughput, storage/shipping conditions, procurement-approval scope, and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
Frequently Asked Questions
Is the SmartDye Terminator v3.1 kit compatible with my existing BigDye workflow? Yes. The SmartDye™ Terminator v3.1 Cycle Sequencing Kit is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit and requires no protocol adjustment, mobility-shift files, or recalibration.
What is in the PR6000-5K pack? The 5,000-reaction pack contains Dye Mix (2×20ml) and 5X Buffer (12ml). Each kit's chemistry comprises fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer.
What can I sequence with it? De novo sequencing, resequencing, and finishing — compatible with PCR products, plasmids, BACs, and fosmids. The optimized dye chemistry gives a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions, with reaction times as short as 3 minutes.
SmartDye pack-size family:
- PR6000-5K (this product) — 5,000 reactions ($39,000; institutional workhorse)
- PR6000-1K — 1,000 reactions ($7,800; entry / validation)
- PR6000-25K — 25,000 reactions ($185,000; genome-center / sequencing-service scale, 5% bulk discount)
Pick the 5K for institutional sequencing operations at mid-volume scale.
