Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, High ROX, 100 Reactions

The PR2122-H-100 is the High ROX formulation of the Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit — sold as 100-reaction entry pack. The multiplex variant of the PR2121 qMAX Probe One-Step RT-qPCR chemistry — engineered for multi-target probe-based RT-qPCR (typical 2–3 targets per reaction with different fluorophore-labeled probes). The multiplex master mix formulation supports the simultaneous amplification + detection of multiple sequence-specific targets in a single tube, with each target's signal tracked through a distinct fluorophore channel on the qPCR cycler.

Universe price BLANK note (FQ-016): The 2026 universe price-list does not enumerate a list price for PR2122-H-100. Confirm pricing at quote. Pricing should be in line with the PR2121 single-target probe chemistry plus a small multiplex premium (the PR2122-N-1000 / PR2121-N-1000 ratio shows $1,566 vs $1,506 — ~4 % multiplex premium at the 1000-rxn pack scale).

Key Specifications

Multiplex RT-qPCR Family Architecture

The Accuris qMAX One-Step RT-qPCR family includes single-target and multiplex chemistry options scaled to multiplex capacity:

Pick the PR2122 Multiplex RT-qPCR (2–3 target) chemistry when:

• Workflow needs 2–3 target multiplexing (typical: target + reference gene, viral target + internal positive control, 3-target diagnostic panel)
• Sequence-specific detection per target with distinct fluorophore probe per target
• Master-mix formulation engineered to handle multiplex efficiency — maximizes per-target sensitivity in multi-primer / multi-probe context

Pick the PR2121 single-target chemistry when: workflow runs only one probe-based target per reaction. Pick the PR2124 4× multiplex when: workflow needs 4-target multiplex panels.

Why a Dedicated Multiplex Master Mix

Standard single-target RT-qPCR master mixes can support 2-target multiplex with reasonable performance, but multi-target multiplex efficiency (3+ primer pairs + 3+ probes in one reaction) typically requires engineered multiplex master mix chemistry:

• Optimized buffer composition for multi-primer-pair amplification efficiency
• Higher polymerase concentration to support multi-target amplification within standard cycle counts
• Hot Start chemistry quality that prevents primer-dimer artifacts when multiple primer pairs are present
• dNTP balance matched to multi-target amplification stoichiometry

The PR2122 Multiplex master mix is engineered for these multi-target requirements.

ROX Formulation Selection

Same as PR2121 / PR2120 / PR2000 / PR2001 family — three ROX-level formulations:

Pack-Size Family

Application Profile

Multi-target pathogen detection panels — workflows running viral/bacterial multiplex panels with target + internal control or multi-strain detection.

Gene expression with reference-gene multiplex — workflows running target gene + reference gene (GAPDH, β-actin, etc.) probes simultaneously for normalization.

Allelic discrimination / genotyping panels — multi-allele probe panels for SNP genotyping.

Clinical-research diagnostic 2–3 target panels — multi-marker diagnostic workflows.

Method-development for multi-target panel design — protocol-development workflows where multi-target amplification efficiency is being optimized.

Multi-investigator clinical-research labs — facilities running standardized multi-target panels.

Ordering & Configuration

Probe inventory. Operator supplies multiple sequence-specific probes (each with different fluorophore label per target).

Cycler-compatibility confirmation. High ROX is for Standard-block ABI 7000-series cyclers AND the cycler must have sufficient fluorophore channels for the multiplex panel (typical 4-channel cyclers support up to 4-target multiplex).

Storage. -20 °C, protect from light. RNase-free handling required.

Pair with RNA template + multiple primer pairs + multiple probes. The kit includes the polymerase + RT + buffer + dNTPs + RNase inhibitor + ROX; operator supplies RNA template, multiple gene-specific primer pairs, and labeled probes per target.

Confirm at quote: cycler ROX requirement, cycler fluorophore-channel count, multiplex panel design (target count + fluorophore channel assignments), workflow throughput, evaluation interest (PR2122-H-S sample), pricing confirmation (universe blank), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris qMAX Probe One-Step RT-qPCR multiplex family architecture:

- PR2121 — Single-target probe-based One-Step RT-qPCR

- PR2122 (this product family) — 2–3 target multiplex probe-based One-Step RT-qPCR

- PR2124 — 4-target multiplex probe-based One-Step RT-qPCR

Pick the PR2122 Multiplex variant for 2–3 target panels; sister chemistries cover single-target and 4-target workflows.