The Accuris PR6000-100 is the 100-reaction entry pack of the SmartDye™ Terminator v3.1 Cycle Sequencing Kit — a cycle sequencing kit based on the Sanger method for DNA sequencing, containing fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer. It is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit — requiring no protocol adjustment, mobility-shift files, or recalibration. This 100-reaction pack supports first-use evaluation or low-throughput Sanger sequencing workflows without the production-scale procurement commitment of the larger 1K / 5K / 25K packs.
Accuris's proprietary optimized dye chemistry delivers a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions. Reaction time is as short as 3 minutes, and the chemistry stays effective at minimal volumes (0.15µl dye in a 5µl total reaction). Applications span de novo sequencing, resequencing, and finishing, and it is compatible with PCR products, plasmids, BACs, and fosmids.
Key Specifications
| SKU | PR6000-100 |
| Full product name | SmartDye™ Terminator v3.1 Cycle Sequencing Kit |
| Type | Sanger cycle sequencing kit — dye-terminator chemistry (entry pack) |
| Pack quantity | 100 reactions |
| Kit contents | Dye Mix 800µl; 5X Buffer 1ml |
| Kit components | Fluorescently labelled dideoxynucleotide terminators, DNA polymerase, 5X optimized buffer |
| Commercial equivalence | Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 — no protocol adjustment, mobility-shift files, or recalibration |
| Reaction time | As short as 3 minutes |
| Minimal reaction volume | Effective at 0.15µl dye in a 5µl total reaction |
| Per-reaction cost | $12.00 (vs $7.80/rxn at 1K pack — 54 % per-rxn premium at small pack scale) |
| Sister pack sizes | PR6000-24 (24 reactions, $450), PR6000-1K (1,000 reactions, $7,800), PR6000-5K (5,000 reactions, $39,000), PR6000-25K (25,000 reactions, $185,000) |
| List price | $1,200 |
SmartDye Pack-Size Family — Per-Reaction Cost Curve
| SKU | Reactions | List price | Per-reaction cost |
| PR6000-24 | 24 | $450 | $18.75 |
| PR6000-100 (this product) | 100 | $1,200 | $12.00 |
| PR6000-1K | 1,000 | $7,800 | $7.80 |
| PR6000-5K | 5,000 | $39,000 | $7.80 |
| PR6000-25K | 25,000 | $185,000 | $7.40 |
Per-reaction economics curve shows steep small-pack premium ($18.75 at 24-rxn, $12.00 at 100-rxn) before stabilizing at production-scale per-rxn cost (~$7.80) at the 1K+ pack tiers — typical sequencing-reagent procurement dynamics where bulk procurement is the cost-optimized choice for production sequencing cores.
Pick the 100-rxn pack when:
- Workflow evaluation — labs evaluating SmartDye sequencing chemistry on a small validation run before committing to the 1K production pack
- Low-throughput Sanger sequencing — labs running occasional Sanger sequencing where 100 reactions covers months of throughput
- Method-development workflows — protocol-development workflows where small-batch sequencing supports primer / template optimization
Application Profile
Sanger sequencing on capillary sequencers — the workhorse application. PCR product, plasmid, BAC, or fosmid template sequencing on capillary sequencer platforms (specific sequencer compatibility is not stated on the manufacturer page — confirm at order).
Method-development workflows — protocol-development sequencing where small-batch reagent procurement supports iterative validation.
Educational laboratory sequencing exercises — molecular-biology coursework introducing Sanger sequencing concepts on a per-class budget.
Low-throughput academic-research labs — labs running occasional Sanger sequencing for clone verification or low-volume sequencing projects.
Ordering & Configuration
Per-reaction workflow consumable. Each Sanger sequencing reaction consumes proportional reagent.
Sequencer-compatibility confirmation. Confirm SmartDye chemistry compatibility with the lab's specific Sanger sequencer (sequencer compatibility is not stated on the manufacturer page — confirm at order).
Storage. Storage conditions are not stated on the manufacturer product page — confirm at order.
Confirm at quote: sequencer compatibility (not stated on the manufacturer page — confirm at order), workflow throughput sizing (justifies entry-pack vs production-scale pack), and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Frequently Asked Questions
Is the SmartDye Terminator v3.1 kit compatible with my existing BigDye workflow? Yes. The SmartDye™ Terminator v3.1 Cycle Sequencing Kit is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit and requires no protocol adjustment, mobility-shift files, or recalibration.
What is in the PR6000-100 pack? The 100-reaction pack contains Dye Mix (800µl) and 5X Buffer (1ml). Each kit's chemistry comprises fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer.
What can I sequence with it? De novo sequencing, resequencing, and finishing — compatible with PCR products, plasmids, BACs, and fosmids. The optimized dye chemistry gives a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions.
Accuris PR6000 SmartDye sequencing reagent family pack-size ladder:
- PR6000-24 — 24 reactions ($450, $18.75/rxn — smallest pack)
- PR6000-100 (this product) — 100 reactions ($1,200, $12.00/rxn — entry pack)
- PR6000-1K — 1,000 reactions ($7,800, $7.80/rxn — production-scale entry)
- PR6000-5K — 5,000 reactions ($39,000, $7.80/rxn)
- PR6000-25K — 25,000 reactions ($185,000, $7.40/rxn — bulk economics)
Pick the 100-reaction entry pack for workflow evaluation or low-throughput Sanger sequencing; step to PR6000-1K for production-scale procurement.
