The Accuris PR6000-25K is the 25,000-reaction bulk pack of the SmartDye™ Terminator v3.1 Cycle Sequencing Kit — the largest pack size in the family. It is a cycle sequencing kit based on the Sanger method, containing fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer, and is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit — requiring no protocol adjustment, mobility-shift files, or recalibration. The 25,000-reaction pack at $185,000 ($7.40 per reaction — modest 5% bulk discount vs the smaller PR6000-1K and PR6000-5K packs at $7.80 per reaction) is sized for genome centers, sequencing service operations, and high-throughput Sanger sequencing workflows where 25,000 reactions per pack-purchase cycle is the operational baseline.
Accuris's proprietary optimized dye chemistry delivers a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions. Reaction time is as short as 3 minutes, and the chemistry stays effective at minimal volumes (0.15µl dye in a 5µl total reaction). Applications span de novo sequencing, resequencing, and finishing, and it is compatible with PCR products, plasmids, BACs, and fosmids.
🚨 Safety flag: HIGH_PRICE_NO_CAPITAL_KW. This SKU's $185,000 price point is well above typical consumable price thresholds — flagged for procurement and class-verification review. Pricing is consistent with bulk-pack scaling within the SmartDye family (25× the 1K pack at modest discount), but the absolute dollar value warrants explicit procurement context confirmation.
Key Specifications
| SKU | PR6000-25K |
| Full product name | SmartDye™ Terminator v3.1 Cycle Sequencing Kit |
| Type | Sanger cycle sequencing kit — dye-terminator chemistry — 25,000 reactions (largest pack in the family) |
| Reactions per kit | 25,000 reactions |
| Kit contents | Dye Mix 10×20ml; 5X Buffer 12ml |
| Kit components | Fluorescently labelled dideoxynucleotide terminators, DNA polymerase, 5X optimized buffer |
| Commercial equivalence | Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 — no protocol adjustment, mobility-shift files, or recalibration |
| Reaction time | As short as 3 minutes |
| Minimal reaction volume | Effective at 0.15µl dye in a 5µl total reaction |
| Per-reaction cost | $7.40 (5% discount vs the 1K/5K packs at $7.80/rxn) |
| List price | $185,000 |
| Safety flag | HIGH_PRICE_NO_CAPITAL_KW (procurement and class-verification review) |
SmartDye Pack-Size Family
The SmartDye family ships in three pack sizes with progressive bulk-pack economics:
| SKU | Reactions | Price | Per-reaction |
| PR6000-1K | 1,000 | $7,800 | $7.80 |
| PR6000-5K | 5,000 | $39,000 | $7.80 |
| PR6000-25K (this product) | 25,000 | $185,000 | $7.40 (5% discount) |
The 25K bulk pack delivers a modest 5% per-reaction discount over the 1K and 5K packs — the bulk-pack economics aren't dramatic, so the 25K pack's value proposition is operational (single procurement cycle covers 25,000 reactions, reducing reorder frequency and ensuring single-lot consistency across workflow batches) rather than purely cost-driven.
When to Order This SKU
Order the PR6000-25K when:
- Genome center or sequencing service operation — high-throughput Sanger sequencing services running >25,000 reactions per quarter benefit from the 25K pack's reduced reorder frequency and single-lot consistency.
- Multi-site or multi-cohort clinical-research studies — workflows requiring a single SmartDye lot across many sites or cohorts to eliminate lot-to-lot variation as a confounder.
- High-volume sequencing service contracts — service operations with committed sequencing volumes that justify upfront procurement of the 25K bulk pack.
- Storage capacity supports the 25K pack — the 25K pack ships substantial reagent volume (Dye Mix 10×20ml plus 5X Buffer 12ml); confirm required storage conditions and volume at order (storage conditions are not stated on the manufacturer page).
For lower-throughput labs or initial validation, the PR6000-1K (1,000 reactions) or PR6000-5K (5,000 reactions) packs are the right pick.
Application Profile
High-throughput Sanger sequencing services — genome centers and academic sequencing core facilities running de novo sequencing, resequencing, and finishing at institutional volume; compatible with PCR products, plasmids, BACs, and fosmids.
Multi-cohort clinical-research validation studies — translational research workflows that require single-lot SmartDye consistency across many cohorts or many sites.
Sequencing-service contracts — commercial sequencing services with committed throughput volumes that justify upfront 25K-reaction procurement.
Genome-wide variant confirmation — sequencing service workflows confirming variants identified by NGS using Sanger validation at high-throughput.
Ordering & Configuration
Confirm at order (per safety-flag note): sequencer platform compatibility and storage conditions (neither is stated on the manufacturer product page — confirm at order), plus expected workflow throughput, storage capacity for the bulk pack, and procurement-class verification ($185K price point warrants explicit class confirmation with Sean). Chemistry and kit composition are confirmed: fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer; the 25K pack ships as Dye Mix 10×20ml and 5X Buffer 12ml.
Storage & handling. Storage conditions are not stated on the manufacturer product page — confirm at order. The 25K bulk pack ships substantial reagent volume, so confirm storage capacity as well.
Confirm at quote: sequencer platform compatibility, expected workflow throughput (25,000-reaction pack appropriate for genome centers and sequencing services; smaller packs for lower-throughput labs), storage/shipping conditions and capacity, procurement-approval scope (the $185K price point may require multi-step institutional approval), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
Frequently Asked Questions
Is the SmartDye Terminator v3.1 kit compatible with my existing BigDye workflow? Yes. The SmartDye™ Terminator v3.1 Cycle Sequencing Kit is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit and requires no protocol adjustment, mobility-shift files, or recalibration.
What is in the PR6000-25K pack? The 25,000-reaction pack contains Dye Mix (10×20ml) and 5X Buffer (12ml). Each kit's chemistry comprises fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer.
What can I sequence with it? De novo sequencing, resequencing, and finishing — compatible with PCR products, plasmids, BACs, and fosmids. The optimized dye chemistry gives a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions, with reaction times as short as 3 minutes.
SmartDye pack-size family:
- PR6000-25K (this product) — 25,000 reactions ($185,000; genome-center / sequencing-service scale, modest bulk discount)
- PR6000-5K — 5,000 reactions ($39,000; institutional workhorse)
- PR6000-1K — 1,000 reactions ($7,800; entry / validation)
Pick the 25K for genome-center or high-volume sequencing-service operations where reorder-frequency reduction and single-lot consistency justify the procurement scale.
