The Accuris PR6000-24 is the 24-reaction pack of the SmartDye™ Terminator v3.1 Cycle Sequencing Kit — a cycle sequencing kit based on the Sanger method for DNA sequencing, containing fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer. It is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit — requiring no protocol adjustment, mobility-shift files, or recalibration. This 24-reaction pack is the smallest pack-size in the family, ideal for first-use evaluation or single-batch low-throughput Sanger sequencing workflows (24-sample plate single-pass sequencing, evaluation runs, method-development).
Accuris's proprietary optimized dye chemistry delivers a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions. Reaction time is as short as 3 minutes, and the chemistry stays effective at minimal volumes (0.15µl dye in a 5µl total reaction). Applications span de novo sequencing, resequencing, and finishing, and it is compatible with PCR products, plasmids, BACs, and fosmids.
Key Specifications
| SKU | PR6000-24 |
| Full product name | SmartDye™ Terminator v3.1 Cycle Sequencing Kit |
| Type | Sanger cycle sequencing kit — dye-terminator chemistry (smallest pack — evaluation / low-throughput) |
| Pack quantity | 24 reactions |
| Kit contents | Dye Mix 200µl; 5X Buffer 1ml |
| Kit components | Fluorescently labelled dideoxynucleotide terminators, DNA polymerase, 5X optimized buffer |
| Commercial equivalence | Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 — no protocol adjustment, mobility-shift files, or recalibration |
| Reaction time | As short as 3 minutes |
| Minimal reaction volume | Effective at 0.15µl dye in a 5µl total reaction |
| Per-reaction cost | $18.75 (highest per-rxn cost in family — small-pack premium) |
| Sister pack sizes | PR6000-100 (100 reactions, $1,200), PR6000-1K (1,000 reactions, $7,800), PR6000-5K (5,000 reactions, $39,000), PR6000-25K (25,000 reactions, $185,000) |
| List price | $450 |
When to Pick the 24-Reaction Pack
| Workflow scenario | Best pack |
| First-use evaluation before committing to production-scale pack | PR6000-24 (this product) |
| Single 24-sample plate sequencing batch | PR6000-24 (this product) |
| Method-development reagent | PR6000-24 or PR6000-100 |
| Low-throughput labs (< 100 reactions / quarter) | PR6000-100 |
| Production-scale Sanger sequencing | PR6000-1K / -5K / -25K |
SmartDye Pack-Size Family — Per-Reaction Cost Curve
| SKU | Reactions | List price | Per-reaction cost |
| PR6000-24 (this product) | 24 | $450 | $18.75 |
| PR6000-100 | 100 | $1,200 | $12.00 |
| PR6000-1K | 1,000 | $7,800 | $7.80 |
| PR6000-5K | 5,000 | $39,000 | $7.80 |
| PR6000-25K | 25,000 | $185,000 | $7.40 |
Per-reaction premium at 24-rxn pack vs production scale: $18.75 vs $7.80 = 2.4× per-rxn cost premium. Justified for evaluation / low-throughput; not justified for production-scale workflows where the bulk packs deliver meaningful per-reaction cost saving.
Application Profile
SmartDye chemistry first-time evaluation — labs evaluating Accuris SmartDye for the first time use the 24-reaction pack for an initial validation run on their specific Sanger sequencer.
Single 24-sample plate sequencing — labs running occasional 24-sample sequencing batches (typical small-research-group clone verification, single-experiment sequencing).
Sequencer-compatibility validation — labs validating SmartDye chemistry on their specific capillary sequencer model before bulk procurement (specific sequencer compatibility is not stated on the manufacturer page — confirm at order).
Method-development reagent workflows — protocol-development workflows where small-batch reagent procurement supports iterative validation.
Educational laboratory sequencing demonstrations — molecular-biology coursework introducing Sanger sequencing on a per-class budget.
Ordering & Configuration
Single-batch / evaluation-pack consumable. 24-reaction pack supports a meaningful Sanger sequencing validation or single-batch run.
Sequencer-compatibility confirmation. Confirm SmartDye chemistry compatibility with the lab's specific Sanger sequencer.
Storage. Storage conditions are not stated on the manufacturer product page — confirm at order.
Confirm at quote: sequencer compatibility (not stated on the manufacturer page — confirm at order), evaluation intent vs production-pack ordering, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Frequently Asked Questions
Is the SmartDye Terminator v3.1 kit compatible with my existing BigDye workflow? Yes. The SmartDye™ Terminator v3.1 Cycle Sequencing Kit is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit and requires no protocol adjustment, mobility-shift files, or recalibration.
What is in the PR6000-24 pack? The 24-reaction pack contains Dye Mix (200µl) and 5X Buffer (1ml). Each kit's chemistry comprises fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer.
What can I sequence with it? De novo sequencing, resequencing, and finishing — compatible with PCR products, plasmids, BACs, and fosmids. The optimized dye chemistry gives a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions.
Accuris PR6000 SmartDye sequencing reagent family pack-size ladder:
- PR6000-24 (this product) — 24 reactions ($450, $18.75/rxn — smallest pack)
- PR6000-100 — 100 reactions ($1,200, $12.00/rxn — entry pack)
- PR6000-1K — 1,000 reactions ($7,800, $7.80/rxn — production-scale entry)
- PR6000-5K — 5,000 reactions ($39,000, $7.80/rxn)
- PR6000-25K — 25,000 reactions ($185,000, $7.40/rxn — bulk economics)
Pick the 24-reaction smallest pack for evaluation or single-batch sequencing; step to production-scale packs for sequencing-core workflows.
