The Accuris PR6000-24 is the 24-reaction pack of the SmartDye™ Terminator v3.1 Cycle Sequencing Kit, a cycle sequencing kit based on the Sanger method for DNA sequencing, containing fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer. It is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit, requiring no protocol adjustment, mobility-shift files, or recalibration. This 24-reaction pack is the smallest pack-size in the family, ideal for first-use evaluation or single-batch low-throughput Sanger sequencing workflows (24-sample plate single-pass sequencing, evaluation runs, method-development).
Accuris's proprietary optimized dye chemistry delivers a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions. Reaction time is as short as 3 minutes, and the chemistry stays effective at minimal volumes (0.15µl dye in a 5µl total reaction). Applications span de novo sequencing, resequencing, and finishing, and it is compatible with PCR products, plasmids, BACs, and fosmids.
| SKU | PR6000-24 |
| Full product name | SmartDye™ Terminator v3.1 Cycle Sequencing Kit |
| Type | Sanger cycle sequencing kit: dye-terminator chemistry (smallest pack: evaluation / low-throughput) |
| Pack quantity | 24 reactions |
| Kit contents | Dye Mix 200µl; 5X Buffer 1ml |
| Kit components | Fluorescently labelled dideoxynucleotide terminators, DNA polymerase, 5X optimized buffer |
| Commercial equivalence | Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 (no protocol adjustment, mobility-shift files, or recalibration) |
| Reaction time | As short as 3 minutes |
| Minimal reaction volume | Effective at 0.15µl dye in a 5µl total reaction |
| Per-reaction cost | $18.75 (highest per-rxn cost in family; small-pack premium) |
| Sister pack sizes | PR6000-100 (100 reactions, $1,200), PR6000-1K (1,000 reactions, $7,800), PR6000-5K (5,000 reactions, $39,000), PR6000-25K (25,000 reactions, $185,000) |
| List price | $450 |
| Workflow scenario | Best pack |
| First-use evaluation before committing to production-scale pack | PR6000-24 (this product) |
| Single 24-sample plate sequencing batch | PR6000-24 (this product) |
| Method-development reagent | PR6000-24 or PR6000-100 |
| Low-throughput labs (< 100 reactions / quarter) | PR6000-100 |
| Production-scale Sanger sequencing | PR6000-1K / -5K / -25K |
| SKU | Reactions | List price | Per-reaction cost |
| PR6000-24 (this product) | 24 | $450 | $18.75 |
| PR6000-100 | 100 | $1,200 | $12.00 |
| PR6000-1K | 1,000 | $7,800 | $7.80 |
| PR6000-5K | 5,000 | $39,000 | $7.80 |
| PR6000-25K | 25,000 | $185,000 | $7.40 |
Per-reaction premium at 24-rxn pack vs production scale: $18.75 vs $7.80 = 2.4× per-rxn cost premium. Justified for evaluation / low-throughput; not justified for production-scale workflows where the bulk packs deliver meaningful per-reaction cost saving.
SmartDye chemistry first-time evaluation. Labs evaluating Accuris SmartDye for the first time use the 24-reaction pack for an initial validation run on their specific Sanger sequencer.
Single 24-sample plate sequencing: labs running occasional 24-sample sequencing batches (typical small-research-group clone verification, single-experiment sequencing).
Sequencer-compatibility validation. Labs validating SmartDye chemistry on their specific capillary sequencer model before bulk procurement (specific sequencer compatibility is not stated on the manufacturer page; confirm at order).
Method-development reagent workflows. Protocol-development workflows where small-batch reagent procurement supports iterative validation.
Educational laboratory sequencing demonstrations. Molecular-biology coursework introducing Sanger sequencing on a per-class budget.
Single-batch / evaluation-pack consumable. 24-reaction pack supports a meaningful Sanger sequencing validation or single-batch run.
Sequencer-compatibility confirmation. Confirm SmartDye chemistry compatibility with the lab's specific Sanger sequencer.
Storage. Storage conditions are not stated on the manufacturer product page. Confirm at order.
Confirm at quote: sequencer compatibility (not stated on the manufacturer page; confirm at order), evaluation intent vs production-pack ordering, and lead time.
Warranty: Per Accuris standard reagent terms. Confirm at quote.
Is the SmartDye Terminator v3.1 kit compatible with my existing BigDye workflow? Yes. The SmartDye™ Terminator v3.1 Cycle Sequencing Kit is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit and requires no protocol adjustment, mobility-shift files, or recalibration.
What is in the PR6000-24 pack? The 24-reaction pack contains Dye Mix (200µl) and 5X Buffer (1ml). Each kit's chemistry comprises fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer.
What can I sequence with it? De novo sequencing, resequencing, and finishing, compatible with PCR products, plasmids, BACs, and fosmids. The optimized dye chemistry gives a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions.
Accuris PR6000 SmartDye sequencing reagent family pack-size ladder:
- PR6000-24 (this product): 24 reactions ($450, $18.75/rxn; smallest pack)
- PR6000-100 (100 reactions ($1,200, $12.00/rxn) entry pack)
- PR6000-1K (1,000 reactions ($7,800, $7.80/rxn) production-scale entry)
- PR6000-5K: 5,000 reactions ($39,000, $7.80/rxn)
- PR6000-25K (25,000 reactions ($185,000, $7.40/rxn) bulk economics)
Pick the 24-reaction smallest pack for evaluation or single-batch sequencing; step to production-scale packs for sequencing-core workflows.
Authorized Distributor
Genuine, factory-warrantied units
Ships from USA
Domestic freight and parcel carriers
Expert Support
Real specialists who know the equipment
Competitive Pricing
Authorized-partner pricing
We are an authorized distributor for Yamato Scientific, Haier Biomedical, Scientemp, Benchmark Scientific, and True Scientific. Every unit comes with full manufacturer warranty and factory support.
Not sure which model fits your lab? Our team speaks your language. We help you spec the right equipment for your exact application and budget.
PO-friendly, quote-ready, and net-term capable. We work with universities, hospitals, and government labs. No friction, no hassle.
Competitive pricing on the page, and we'll match any legitimate competitor price. You get premium equipment without the premium markup.
Direct to your dock. You get the latest stock straight from the source. If we don't have inventory, we work with the manufacturer to get what you need.
Legacy Lab Supplies isn't just a transaction. We're building a long-term relationship with every lab we serve, from first quote to repeat orders.
Accuris qMAX™ Multiplex One-Step 4X RT-qPCR Kit, No ROX, 2,000 Reactions
$1,708.36
Accuris SmartDye™ Terminator v3.1 Cycle Sequencing Kit (PR6000-100, 100 reactions)
Price on request
Accuris SmartDye™ Terminator v3.1 Cycle Sequencing Kit (PR6000-25K, 25,000 reactions)
$185,000.00
Accuris SmartDye™ Terminator v3.1 Cycle Sequencing Kit (PR6000-5K, 5,000 reactions)
$39,000.00
Accuris qMAX™ Multiplex One-Step 4X RT-qPCR Kit, No ROX, 2,000 Reactions
$1,708.36
Accuris qMAX Probe Multiplex One-Step RT-qPCR Kit, High ROX, Sample (PR2122-H-S, 20 Reactions)
Price on request
Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, High ROX, 100 Reactions
Price on request
Accuris qMAX Probe One-Step Multiplex RT-qPCR Kit, High ROX, 500 Reactions
Price on request
Scientemp Model 34-15 Chest Freezer 0°C to-34°C with 15 Cu. Ft. Capacity
Price on request
Scientemp Model 34-20 Chest Freezer 0°C to-34°C with 20 Cu. Ft. Capacity
Price on request
Scientemp Model 34-25 Chest Freezer 0°C to-34°C with 25 Cu. Ft. Capacity
Price on request
Scientemp Model 40-9.4 Chest Freezer 0°C to-40°C with 9.4 Cu. Ft. Capacity
Price on request
