The Accuris PR6000-1K is the 1,000-reaction pack of the SmartDye™ Terminator v3.1 Cycle Sequencing Kit, a cycle sequencing kit based on the Sanger method for DNA sequencing, containing fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer. It is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit, requiring no protocol adjustment, mobility-shift files, or recalibration. The 1,000-reaction pack at $7,800 ($7.80 per reaction) is the production-scale entry point in the SmartDye family, useful for lower-throughput sequencing labs or for initial workflow validation before committing to the larger 5K or 25K bulk packs.
Accuris's proprietary optimized dye chemistry delivers a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions. Reaction time is as short as 3 minutes, and the chemistry stays effective at minimal volumes (0.15µl dye in a 5µl total reaction). Applications span de novo sequencing, resequencing, and finishing, and it is compatible with PCR products, plasmids, BACs, and fosmids.
Key Specifications
| SKU | PR6000-1K |
| Full product name | SmartDye™ Terminator v3.1 Cycle Sequencing Kit |
| Type | Sanger cycle sequencing kit: dye-terminator chemistry: 1,000 reactions |
| Reactions per kit | 1,000 reactions |
| Kit contents | Dye Mix 4×2ml; 5X Buffer 12ml |
| Kit components | Fluorescently labelled dideoxynucleotide terminators, DNA polymerase, 5X optimized buffer |
| Commercial equivalence | Direct replacement for Applied Biosystems™ BigDye™ Terminator v3.1 (no protocol adjustment, mobility-shift files, or recalibration) |
| Reaction time | As short as 3 minutes |
| Minimal reaction volume | Effective at 0.15µl dye in a 5µl total reaction |
| Per-reaction cost | $7.80 (linear with 5K pack at $7.80/rxn; slight 5% discount at 25K pack tier at $7.40/rxn) |
| List price | $7,800 |
SmartDye Pack-Size Family
The SmartDye family ships in three pack sizes with bulk-pack economics:
| SKU | Reactions | Price | Per-reaction |
| PR6000-1K (this product) | 1,000 | $7,800 | $7.80 |
| PR6000-5K | 5,000 | $39,000 | $7.80 |
| PR6000-25K | 25,000 | $185,000 | $7.40 |
Pricing scales linearly from 1K to 5K (no bulk discount at 5K tier) but offers a modest 5% per-reaction discount at the 25K bulk tier. The 1K pack is the right pick for lower-throughput labs or for initial workflow validation; the 5K pack is the workhorse for institutional-scale Sanger sequencing operations; the 25K pack is the right pick for genome centers or high-throughput sequencing services.
When to Order This SKU
Order the PR6000-1K when:
- Lower-throughput Sanger sequencing workflow. Academic labs, small clinical-research labs, or specialty applications running <1,000 sequencing reactions per pack-purchase cycle.
- Initial workflow validation. Testing the SmartDye chemistry against the lab's existing sequencing workflow before committing to the larger bulk packs.
- Specialty sequencing applications. Workflows where the SmartDye chemistry supports specific protocol requirements that justify the per-reaction premium.
For higher-throughput labs, the PR6000-5K (5,000 reactions) or PR6000-25K (25,000 reactions) pack delivers institutional procurement scale.
Application Profile
Sanger sequencing workflows. De novo sequencing, resequencing, and finishing across academic and clinical-research labs, sequencing service core facilities, and specialty Sanger applications; compatible with PCR products, plasmids, BACs, and fosmids.
Cycle sequencing reactions. Typical Sanger workflow with cycle sequencing on real-time thermal cyclers followed by sequencer-based capillary electrophoresis read-out.
Bulk-pack procurement. The 1K pack serves lower-throughput needs; for institutional sequencing volume, the 5K or 25K bulk packs are the right pick.
Ordering & Configuration
Confirm at order: sequencer platform compatibility and storage conditions (neither is stated on the manufacturer product page; confirm at order), plus expected workflow throughput and any cycler-specific considerations. Chemistry and kit composition are confirmed: fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer; the 1K pack ships as Dye Mix 4×2ml and 5X Buffer 12ml.
Storage & handling. Storage conditions are not stated on the manufacturer product page. Confirm at order.
Confirm at quote: sequencer platform compatibility, expected workflow throughput, storage/shipping conditions, and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms. Confirm at quote.
Frequently Asked Questions
Is the SmartDye Terminator v3.1 kit compatible with my existing BigDye workflow? Yes. The SmartDye™ Terminator v3.1 Cycle Sequencing Kit is a direct replacement for the Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit and requires no protocol adjustment, mobility-shift files, or recalibration.
What is in the PR6000-1K pack? The 1,000-reaction pack contains Dye Mix (4×2ml) and 5X Buffer (12ml). Each kit's chemistry comprises fluorescently labelled dideoxynucleotide terminators, DNA polymerase, and a 5X optimized buffer.
What can I sequence with it? De novo sequencing, resequencing, and finishing, compatible with PCR products, plasmids, BACs, and fosmids. The optimized dye chemistry gives a high signal-to-noise ratio and extended read lengths, especially in GC-rich regions, with reaction times as short as 3 minutes.
SmartDye pack-size family:
- PR6000-1K (this product): 1,000 reactions ($7,800; entry / validation)
- PR6000-5K: 5,000 reactions ($39,000; institutional workhorse)
- PR6000-25K: 25,000 reactions ($185,000; genome-center / sequencing-service scale)
Pick the 1K for lower-throughput or initial validation; pick 5K or 25K for institutional sequencing volume.
