The Yamato ONC-362DS001G is the On-chip Droplet Selector HSG, a microfluidic instrument that identifies, sorts, and dispenses specific water-in-oil (W/O) droplets containing cells or microorganisms. It enables high-throughput, damage-free screening of encapsulated samples and dispenses individual selected droplets directly into 96- or 384-well plates, one droplet at a time. This HSG configuration is equipped with 488 nm, 561 nm, and 405 nm excitation lasers — the 561 nm line adds efficient excitation of orange/red-shifted reporters such as mCherry, DsRed, and PE.
Key Specifications
| SKU | ONC-362DS001G |
| Product | On-chip Droplet Selector HSG |
| Detection lasers | 488 nm, 561 nm, 405 nm |
| Sample format | Water-in-oil (W/O) droplets encapsulating cells or microorganisms |
| Function | Identify, sort, and dispense selected droplets |
| Dispensing | Direct into 96- or 384-well plates, one droplet at a time |
| Handling | High-throughput, damage-free (gentle) screening |
| List price | Contact for pricing (TBA) |
Application Profile
In droplet microfluidics each water-in-oil droplet is an isolated picoliter reactor — one cell and its assay, sealed off from every neighbor — which is why it has become a backbone of single-cell and single-microorganism screening. The hard part is recovering the *right* droplet intact. The On-chip Droplet Selector reads each droplet's fluorescence on the fly, decides whether it matches the target, and dispenses the winners into a defined well without harming their contents.
The damage-free dispensing means cells or microorganisms in a selected droplet arrive viable and ready for clonal expansion, sequencing, or culture. Dispensing one droplet at a time into addressable 96- or 384-well plates supports single-cell isolation, antibody-discovery screens, directed-evolution selections, and rare-variant enrichment. The HSG laser set (488/561/405 nm) is the choice when your panel leans on 561 nm-excited fluorophores rather than the far-red 638 nm line.
Frequently Asked Questions
What does the On-chip Droplet Selector do? It identifies, sorts, and dispenses specific water-in-oil (W/O) droplets containing cells or microorganisms, placing selected droplets directly into well plates.
Which lasers does this HSG model have? The HSG (ONC-362DS001G) is equipped with 488 nm, 561 nm, and 405 nm excitation lasers.
When should I pick the 561 nm (HSG) configuration? Choose HSG when your reporters are 561 nm-excited — orange/red-shifted dyes and proteins such as mCherry, DsRed, or PE — rather than far-red probes best matched to 638 nm.
Where are the sorted droplets dispensed? Directly into 96- or 384-well plates, one droplet at a time, so each well receives a defined, selected droplet.
Is the sorting gentle on cells? Yes — damage-free dispensing keeps encapsulated cells or microorganisms intact for downstream culture, sequencing, or analysis.
How do the HS, HSG, and HSGR versions differ? Only by their laser configuration. HSG uses 488/561/405 nm; HS (ONC-362DS001) uses 488/638/405 nm; HSGR (ONC-362DS001GR) uses 488/638/561 nm.
Ordering & Configuration
Confirm at quote: your fluorophore panel (to select HS vs HSG vs HSGR), target plate format (96 vs 384), and chip/consumable supply.
Warranty: Per Yamato standard capital-equipment terms — confirm coverage at quote.
Add to a quote to request current pricing and lock configuration and lead time.
On-chip Droplet Selector variants (laser set is the only difference):
- HSG — ONC-362DS001G (this product) — 488 / 561 / 405 nm
- HS — ONC-362DS001 — 488 / 638 / 405 nm
- HSGR — ONC-362DS001GR — 488 / 638 / 561 nm
Pick the variant whose excitation lines match your reporters; the sorting, dispensing, and damage-free handling are identical across all three.
