isoHub Single-Cell Imaging & Verification System, Monoclonality Assurance

The Yamato IOTA-10060 is the isoHub, a smart automated imaging system built to verify single-cell monoclonality and track clonal outgrowth within GRID-based cultures. After cells are plated into GRID chambers, the isoHub automates navigation through the GRIDs and lets you select the chamber coordinates of the single cells to isolate. Its optics let you view entire GRID chambers without optical edge effects, giving clear-cut identification of single cells, and it communicates wirelessly with the isoPick so the imaging and picking steps stay in sync.

Key Specifications

Application Profile

For clonal cell-line development (especially for biologics and therapeutic cell lines) regulators and quality systems demand documented assurance that a colony arose from a single cell. That "proof of monoclonality" is often the rate-limiting, evidence-heavy step of the whole workflow. The isoHub is built to supply it: after plating into GRID chambers, it automatically navigates the grid and images each chamber, so you can identify true single cells and record the evidence rather than infer clonality statistically.

The edge-effect-free imaging is the technical differentiator. Optical distortion at the boundaries of a well or chamber is a classic reason single cells get missed or miscounted; by viewing entire GRID chambers without those edge effects, the isoHub gives clear-cut identification across the full field. It then tracks clonal outgrowth over time, documenting that the colony descended from the verified single cell. Paired with the isoPick over a wireless link, the two form a closed loop (image and verify on the isoHub, pick on the isoPick) that streamlines the single-cell isolation process end to end.

Frequently Asked Questions

What does the isoHub do? It is an automated imaging system that verifies single-cell monoclonality and tracks clonal outgrowth in GRID-based cultures.

How does it help identify single cells? It automates navigation through the GRID chambers and lets you select chamber coordinates, imaging entire chambers without optical edge effects for clear-cut single-cell identification.

Why does monoclonality verification matter? Clonal cell-line development, particularly for biologics, requires documented proof that a colony came from one cell. isoHub provides the imaging evidence and outgrowth tracking to support that.

Does it connect to the isoPick? Yes, the isoHub communicates wirelessly with the isoPick (IOTA-20010), keeping imaging/verification and picking in sync.

What culture format does it use? GRID-based cultures, cells plated into GRID chambers that the isoHub can navigate and image systematically.

Can I use it on its own? The isoHub handles imaging and verification; it is designed to work alongside the isoPick for the isolation step. Together they form a complete pick-and-verify workflow.

Ordering & Configuration

Confirm at quote: whether you're pairing with an existing or new isoPick, your monoclonality-documentation requirements, and GRID consumable supply.

Warranty: Per Yamato standard capital-equipment terms. Confirm coverage at quote.

Add to a quote to lock configuration and lead time.

Single-cell isolation pair:

- isoHub: IOTA-10060 (this product): automated imaging that verifies monoclonality and navigates the GRID.

- isoPick, IOTA-20010, gently isolates and transfers single cells into plates, PCR tubes, or MS vials.

Choose the isoHub when documented monoclonality assurance is the requirement; pair it with the isoPick over the wireless link for a seamless image-verify-pick workflow.