The P7700-1000 is the 100 to 1,000 µL high-microliter variable-volume pipette in the Accuris NextPette P7700 family — the workhorse pipette for buffer transfer, sample resuspension, cell-culture media handling, magnetic-bead workflow buffer additions, and other workflow steps in the high-microliter to ~1 mL range.
Per FQ-017 in fred_followup_queue.md, the NextPette brochure is not in the available corpus; spec details are confirmed from universe label only.
Voltage options: This unit can be ordered with European voltage requirements (230V/50Hz). Contact us to order.
Key Specifications
| SKU | P7700-1000 |
| Type | Manual variable-volume pipette |
| Volume range | 100 µL to 1,000 µL (high-microliter) |
| Family position | High-microliter range — between P7700-200 (20–200 µL) and P7700-5M (500 µL – 5 mL) |
| Workflow context | Buffer transfer, sample resuspension, cell-culture media handling, magnetic-bead workflow buffer additions |
Why the 100–1,000 µL Range Is the High-Volume Workhorse
The 100–1,000 µL range covers most standard buffer-transfer and sample-handling pipetting workflows:
| Workflow | Typical 100–1,000 µL pipetting steps |
| Plasmid mini-prep | Resuspension buffer (250 µL), lysis buffer (250 µL), neutralization buffer (350 µL), elution buffer (50–100 µL — at bottom of range) |
| Magnetic-bead extraction | Lysis buffer (200–500 µL), wash buffer (500–1,000 µL), elution volume (50–100 µL) |
| Cell-culture media handling | Sub-culture media transfers (500–1,000 µL), trypsin addition (500–1,000 µL) |
| Sample resuspension | Resuspension volumes (200–1,000 µL) for cell pellets, DNA / RNA precipitates |
| PBS / TBS rinses | Wash buffer additions for ELISA / immunoassay workflows (200–1,000 µL) |
| DNA / RNA precipitation | Ethanol additions (typical 2.5× sample volume — often in the 500–1,000 µL range) |
The P7700-1000 is one of the most-used pipettes in molecular-biology bench setups alongside the P7700-10 (low-microliter workhorse) and P7700-100 (mid-microliter workhorse).
Application Profile
Plasmid mini-prep workflows — the workhorse application. Standard mini-prep buffer additions (resuspension / lysis / neutralization / wash / elution) all fall within or near the P7700-1000 range.
Magnetic-bead nucleic-acid extraction — wash buffer and elution buffer addition workflows.
Cell-culture media handling — sub-culture media transfers and trypsin addition workflows.
Sample resuspension workflows — DNA / RNA pellet resuspension at standard 100–500 µL volumes.
ELISA / immunoassay wash workflows — wash buffer additions at 200–500 µL per well.
Multi-pipette laboratory equipping — labs equipping new bench setups consider the P7700-10 + P7700-100 + P7700-1000 trio the standard three-pipette starter set covering 0.5–1,000 µL with reasonable accuracy across the range.
Frequently Asked Questions
What volume range does the P7700-1000 cover? 100 µL to 1,000 µL — the high-microliter range covering most buffer-transfer and sample-handling steps.
What pipette tips does the P7700-1000 use? 1,000 µL low-retention pipette tips; confirm tip-format compatibility at order.
What is the standard NextPette starter set? The P7700-10 + P7700-100 + P7700-1000 trio, covering roughly 0.5–1,000 µL with reasonable accuracy across the range.
Which workflows is the P7700-1000 used for? Buffer transfer, sample resuspension, cell-culture media handling, and magnetic-bead workflow buffer additions in the 100–1,000 µL range.
Ordering & Configuration
Pipette tip pairing. The P7700-1000 typically uses 1,000 µL low-retention pipette tips; confirm tip-format compatibility at order.
Calibration and maintenance. Same calibration program as the rest of the NextPette family.
Workflow-priority sequencing in lab equipping. When equipping a new molecular-biology bench, the P7700-10 + P7700-100 + P7700-1000 trio typically covers the majority of low-to-high microliter workflow needs. Add P7700-1 for sub-microliter accuracy; add P7700-20 / P7700-200 for overlap-range accuracy optimization; add P7700-5M / P7700-10M for macro-volume handling.
Confirm at quote: workflow buffer / sample / resuspension pipetting needs in the 100–1,000 µL range, full NextPette family equipping plan, tip-format pairing, calibration program, and lead time.
Warranty: Per Accuris standard warranty terms — confirm at quote.
Accuris NextPette P7700 manual variable-volume pipette family:
- P7700-1 — 0.1–1.0 µL
- P7700-10 — 0.5–10 µL
- P7700-20 — 2–20 µL
- P7700-100 — 10–100 µL
- P7700-200 — 20–200 µL
- P7700-1000 (this product) — 100–1,000 µL — high-microliter workhorse
- P7700-5M — 500 µL – 5 mL
- P7700-10M — 1–10 mL
Pick the P7700-1000 as the third member of the standard P7700-10 + P7700-100 + P7700-1000 starter trio for new lab equipping.
