The P7700-100 is the 10 to 100 µL mid-microliter variable-volume pipette in the Accuris NextPette P7700 family — the workhorse pipette for buffer aliquoting, cell-suspension aliquoting, sample loading, and other mid-microliter pipetting workflows. The 10–100 µL range covers the standard reaction-component-volume range for most molecular-biology master mix preparations, gel-loading volumes, and cell-culture sample handling.
Author confidence note (MEDIUM): Same as sister P7700 SKUs — NextPette brochure is not in the available corpus; specifications confirmed from universe label. Specific calibration accuracy / precision specs to confirm at first order.
Voltage options: This unit can be ordered with European voltage requirements (230V/50Hz). Contact us to order.
Key Specifications
| SKU | P7700-100 |
| Type | Manual variable-volume pipette |
| Volume range | 10 µL to 100 µL (mid-microliter) |
| Family position | Mid-range in the NextPette P7700 family |
| Workflow context | Mid-microliter pipetting — buffer aliquoting, sample loading, cell-suspension aliquoting |
| List price | $283 |
Why the 10–100 µL Range Is the Mid-Volume Workhorse
The 10–100 µL range covers a high density of standard pipetting steps in molecular-biology and cell-biology workflows:
| Workflow | Typical 10–100 µL pipetting steps |
| Master mix preparation (multi-reaction mix) | Buffer (10–50 µL), water (variable), aliquoting per-reaction master mix volumes (10–25 µL) |
| Gel loading | Sample load volumes (10–25 µL); loading dye additions (5 µL — at bottom of P7700-100 range) |
| DNA elution | Elution buffer volumes (30–100 µL) |
| Cell-suspension aliquoting | Cell-counting sample volumes (10–20 µL); culture aliquots (50–100 µL) |
| Magnetic-bead extraction | Wash buffer aliquots (50–100 µL), elution volumes (50–100 µL) |
| Standard ELISA / immunoassay | Sample addition (50–100 µL), wash buffer (50–100 µL), substrate addition (100 µL) |
A typical molecular-biology / cell-biology bench setup runs the P7700-100 alongside the P7700-10 as the two highest-utilization pipettes in the NextPette family.
NextPette Volume-Range Family
| SKU | Volume range | List price |
| P7700-1 | 0.1 – 1.0 µL | $311 |
| P7700-10 | 0.5 – 10 µL | $283 |
| P7700-20 | 2 – 20 µL | (sister SKU) |
| P7700-100 (this product) | 10 – 100 µL | $283 |
| P7700-1000 | 100 – 1,000 µL | (sister SKU) |
| P7700-10M | 1 – 10 mL | (macro-volume) |
P7700-100 vs P7700-20 overlap. The P7700-100 (10–100 µL) and P7700-20 (2–20 µL) overlap in the 10–20 µL range. Pick the P7700-20 for higher accuracy in the 10–20 µL range (a 2–20 µL pipette at 10 µL is operating at 50 % of its range — better accuracy than the P7700-100 at 10 µL operating at 10 % of its range).
Application Profile
Master mix preparation workflows — the workhorse application. Multi-reaction master mix preparation with buffer + reagent additions in the 10–100 µL range.
Gel loading workflows — DNA agarose gel and SDS-PAGE protein gel loading at standard 10–25 µL sample volumes.
DNA elution from spin columns / magnetic beads — elution buffer addition and elution-volume aspiration at the 30–100 µL range.
ELISA / immunoassay workflows — sample addition, wash buffer addition, substrate addition at the 50–100 µL range.
Cell-counting sample aliquoting — cell suspension aliquoting for QuadCount or hemocytometer counting at the 10–20 µL range.
Multi-pipette laboratory equipping — labs equipping new bench setups consider the P7700-100 the second-priority pipette purchase after P7700-10 (highest workflow utilization).
Frequently Asked Questions
What volume range does the P7700-100 cover? 10 µL to 100 µL — the mid-microliter range that covers most reaction-component, gel-loading, and cell-suspension aliquoting steps.
What pipette tips does the P7700-100 use? 200 µL low-retention pipette tips; confirm tip-format compatibility at order.
Should I pick the P7700-100 or the P7700-20 for 10–20 µL work? Pick the P7700-20 for higher accuracy in the 10–20 µL overlap range — a 2–20 µL pipette at 10 µL runs at 50 % of its range versus the P7700-100 at 10 % of its range.
How often does the P7700-100 need calibration? Same program as the NextPette family — a typical 6–12 month calibration interval per ISO 8655.
Ordering & Configuration
Pipette tip pairing. The P7700-100 typically uses 200 µL low-retention pipette tips; confirm tip-format compatibility at order. Tip procurement is operator-supplied per workflow.
Calibration and maintenance. Same calibration program as the rest of the NextPette family — typical 6–12 month calibration interval per ISO 8655.
Workflow-priority sequencing in lab equipping. When equipping a new molecular-biology / cell-biology bench, the P7700-10 + P7700-100 pair typically covers the majority of low-to-mid microliter workflow needs. Add P7700-1000 for higher-volume buffer / sample transfer; add P7700-1 for sub-microliter accuracy needs.
Confirm at quote: workflow buffer / sample / loading pipetting needs, full NextPette family equipping plan, tip-format pairing, calibration program, and lead time.
Warranty: Per Accuris standard warranty terms — confirm at quote.
Accuris NextPette P7700 manual variable-volume pipette family:
- P7700-1 — 0.1–1.0 µL (sub-microliter, $311)
- P7700-10 — 0.5–10 µL (low-microliter workhorse, $283)
- P7700-20 — 2–20 µL
- P7700-100 (this product) — 10–100 µL (mid-microliter workhorse, $283)
- P7700-1000 — 100–1,000 µL
- P7700-10M — 1–10 mL (macro-volume)
Pick the P7700-100 as the second-priority pipette purchase for new lab equipping; pair with P7700-10 for the standard low-to-mid microliter workflow.
