The P7700-20 is the 2 to 20 µL sub-low-microliter variable-volume pipette in the Accuris NextPette P7700 family — used for PCR template addition (1–5 µL), enzyme aliquoting (1–2 µL), gel loading dye addition (5 µL), and other workflow steps where the 2–20 µL range delivers better accuracy than the broader P7700-10 (0.5–10 µL) or P7700-100 (10–100 µL) at the bottom or top of their ranges. Per FQ-017 in fred_followup_queue.md, the NextPette brochure is not in the available corpus; spec details are confirmed from universe label only.
Voltage options: This unit can be ordered with European voltage requirements (230V/50Hz). Contact us to order.
Key Specifications
| SKU | P7700-20 |
| Type | Manual variable-volume pipette |
| Volume range | 2 µL to 20 µL (sub-low-microliter) |
| Family position | Between P7700-10 (0.5–10 µL) and P7700-100 (10–100 µL) — overlap range at 2–20 µL |
| Workflow context | PCR template addition, enzyme aliquoting, gel loading dye addition |
P7700-20 vs P7700-10 vs P7700-100 — Pick the Right Pipette in the Overlap Range
The NextPette P7700 family includes three pipettes covering the 0.5–100 µL range with overlapping ranges for accuracy optimization:
| Pipette | Volume range | Best at | Trade-off |
| P7700-10 | 0.5 – 10 µL | Top accuracy at 1–10 µL — bottom of range (~0.5 µL) operates at 5% of range with reduced accuracy | Range floor below 1 µL is at the bottom of accuracy spec |
| P7700-20 (this product) | 2 – 20 µL | Top accuracy at 5–20 µL — workhorse for the 5–20 µL band | Range floor at 2 µL operates at 10 % of range with moderate accuracy |
| P7700-100 | 10 – 100 µL | Top accuracy at 25–100 µL — bottom of range (10 µL) operates at 10 % of range with moderate accuracy | Range floor at 10 µL is at the bottom of accuracy spec |
Pick the P7700-20 when:
- The workflow's primary pipetting volumes are in the 5–20 µL range (PCR template addition, enzyme aliquoting, gel loading) — operating at 25–100 % of range delivers best accuracy
- The lab needs higher accuracy at 10 µL than the P7700-10 (top of range) or P7700-100 (bottom of range) can deliver
Skip the P7700-20 when: the workflow's primary volumes are sub-microliter (use P7700-10), or > 20 µL (use P7700-100).
Application Profile
PCR template addition — the workhorse application. Standard 25 µL PCR reactions use 1–5 µL of template; the P7700-20 delivers accurate template addition.
Enzyme aliquoting — restriction enzymes, ligases, polymerases at 0.5–2 µL per reaction.
Gel loading dye addition — typical 5 µL loading dye additions to 20 µL gel-loading samples.
Probe / primer aliquoting in qPCR setup — qPCR probes and primers at 1–2 µL per reaction.
Sequencing reaction setup — Sanger sequencing reactions with 1–8 µL template addition.
Multi-pipette laboratory equipping — labs equipping new bench setups consider the P7700-20 a complement to the P7700-10 / P7700-100 pair when 5–20 µL workflow accuracy matters.
Frequently Asked Questions
What is the volume range of the P7700-20? 2 µL to 20 µL.
Which pipette tips does it use? Typically 20 µL low-retention tips; confirm tip-format compatibility at order.
When should I pick the P7700-20 over the P7700-10 or P7700-100? Choose it for best accuracy in the 5–20 µL band. Use the P7700-10 (0.5–10 µL) for sub-microliter work and the P7700-100 (10–100 µL) above 20 µL.
What is the recommended calibration interval? Typically 6–12 months per ISO 8655, on the same program as the rest of the NextPette family.
What workflows is it used for? PCR template addition, enzyme aliquoting, and gel loading dye addition.
Ordering & Configuration
Pipette tip pairing. The P7700-20 typically uses 20 µL low-retention pipette tips; confirm tip-format compatibility at order.
Calibration and maintenance. Same calibration program as the rest of the NextPette family — typical 6–12 month interval per ISO 8655.
Confirm at quote: workflow PCR / enzyme / gel-loading pipetting needs in the 2–20 µL range, full NextPette family equipping plan, tip-format pairing, calibration program, and lead time.
Warranty: Per Accuris standard warranty terms — confirm at quote.
Accuris NextPette P7700 manual variable-volume pipette family:
- P7700-1 — 0.1–1.0 µL
- P7700-10 — 0.5–10 µL
- P7700-20 (this product) — 2–20 µL
- P7700-100 — 10–100 µL
- P7700-200 — 20–200 µL
- P7700-1000 — 100–1,000 µL
- P7700-5M — 500 µL – 5 mL
- P7700-10M — 1–10 mL
Pick the P7700-20 for top accuracy in the 5–20 µL band; sister pipettes cover the rest of the manual-pipette volume range.
