The Accuris NextPette Variable Volume Pipette is the manual variable-volume pipette family for routine bench-pipetting workflows. The P7700-1 is the smallest volume-range model in the family — covering 0.1 µL to 1.0 µL — used for the sub-microliter pipetting steps common in sequencing reactions, qPCR component aliquoting, low-input library prep, and other protocol steps requiring sub-microliter accuracy.
Author confidence note (MEDIUM): The NextPette brochure is not present in the available Accuris brochure corpus; this page is authored from the universe label and standard manual-pipette workflow knowledge. Specific calibration accuracy, precision specifications, ergonomic features, and ISO 8655 conformance details should be confirmed with the NextPette product literature or Accuris technical support at first order.
Voltage options: This unit can be ordered with European voltage requirements (230V/50Hz). Contact us to order.
Key Specifications
| SKU | P7700-1 |
| Type | Manual variable-volume pipette |
| Volume range | 0.1 µL to 1.0 µL (sub-microliter) |
| Family position | Smallest-volume model in the NextPette P7700 family |
| Workflow context | Sub-microliter pipetting — sequencing reactions, qPCR component aliquoting, low-input library prep |
NextPette Volume-Range Family
The Accuris NextPette P7700 family spans the standard manual-pipette volume range across multiple SKUs:
| SKU | Volume range | Use case |
| P7700-1 (this product) | 0.1 – 1.0 µL | Sub-microliter — sequencing reactions, qPCR component aliquoting |
| P7700-10 | 0.5 – 10 µL | Low-microliter — PCR setup, NGS library prep, master mix aliquoting |
| P7700-20 | 2 – 20 µL | Standard low-volume — PCR template addition, sample loading for gels |
| P7700-100 | 10 – 100 µL | Standard mid-volume — buffer aliquoting, cell-suspension aliquoting |
| P7700-1000 | 100 – 1,000 µL | Standard high-volume — buffer transfer, sample resuspension |
| P7700-10M | 1 – 10 mL | Macro-volume — bulk reagent transfer, media aliquoting |
Pick the P7700-1 (0.1–1.0 µL) when the workflow includes sub-microliter pipetting steps that exceed the accuracy of larger-volume pipettes operating at the bottom of their range.
Why a Dedicated Sub-Microliter Pipette
Standard variable-volume pipettes typically lose accuracy near the bottom of their volume range — a 2–20 µL pipette set to 1 µL is operating at 5% of its range, where mechanical tolerances and surface-tension effects degrade accuracy compared to a dedicated 0.1–1.0 µL pipette operating at 100% of its range.
Workflows that benefit from a dedicated 0.1–1.0 µL pipette:
- Sanger sequencing reaction setup — sequencing reactions typically use 0.5–1.0 µL of primer per reaction; accurate sub-microliter pipetting prevents under- or over-concentration of primer
- qPCR component aliquoting — qPCR master mixes contain components (probe, primer, template) at sub-microliter per-reaction volumes
- NGS library prep — adapter ligation and barcoding steps use sub-microliter adapter / index volumes
- Low-input cell suspensions — single-cell or low-cell-count sample handling requires precise sub-microliter aspiration
- Mass spectrometry sample prep — MS injection volumes and standard preparation require accurate sub-microliter pipetting
- PCR mutagenesis workflows — site-directed mutagenesis primer addition
Application Profile
Sequencing core sample preparation — sequencing facilities running Sanger and NGS workflows use dedicated sub-microliter pipettes for protocol-step accuracy.
qPCR setup workflows — qPCR component aliquoting where sub-microliter volumes feed into 10–25 µL final reaction volumes.
NGS library prep facilities — library construction workflows with sub-microliter adapter / barcode addition steps.
Pharma / biotech assay development — assay-development workflows with sub-microliter component titration.
Educational laboratory pipetting practice — molecular-biology coursework demonstrating the volume-range hierarchy in pipette selection.
Multi-pipette laboratory equipping — labs equipping new bench setups benefit from a complete NextPette family covering the full 0.1 µL – 10 mL range.
Frequently Asked Questions
What volume range does the P7700-1 cover? 0.1 µL to 1.0 µL — the sub-microliter range and the smallest model in the NextPette P7700 family.
Why use a dedicated sub-microliter pipette instead of a larger one set low? Larger pipettes lose accuracy near the bottom of their range; a dedicated 0.1–1.0 µL pipette operates at 100% of its range for better sub-microliter accuracy.
What tips does the P7700-1 use? Matching low-volume tips — typically 10 µL low-retention tips; confirm tip-format compatibility at order.
How often should the P7700-1 be calibrated? Per ISO 8655 or institutional standard, typically every 6–12 months depending on use intensity; the AccuCheck system (W3002A-120ACS) supports in-lab calibration.
What workflows is the P7700-1 suited for? Sub-microliter steps such as sequencing reaction setup, qPCR component aliquoting, and NGS library prep.
Ordering & Configuration
Pipette tip pairing. Sub-microliter pipettes require matching low-volume pipette tips — confirm tip-format compatibility at order (typical 0.1–1.0 µL pipettes use 10 µL low-retention tips). Tip procurement is operator-supplied per workflow.
Calibration and maintenance. Manual pipettes require periodic calibration per ISO 8655 (or institutional standard) — typical calibration interval is 6–12 months depending on use intensity. Confirm calibration program at order; AccuCheck pipette calibration system (W3002A-120ACS) is a paired Accuris solution for in-lab calibration.
Confirm at quote: workflow sub-microliter pipetting needs, full NextPette family equipping (single-pipette vs full-range purchase), tip-format pairing, calibration program, and lead time.
Warranty: Per Accuris standard warranty terms — confirm at quote.
Accuris NextPette P7700 manual variable-volume pipette family:
- P7700-1 (this product) — 0.1–1.0 µL (sub-microliter)
- P7700-10 — 0.5–10 µL
- P7700-20 — 2–20 µL
- P7700-100 — 10–100 µL
- P7700-1000 — 100–1,000 µL
- P7700-10M — 1–10 mL (macro-volume)
Pick the P7700-1 for sub-microliter pipetting accuracy; size the full pipette family to the workflow's volume-range needs.
