The P7700-10 is the 0.5 to 10 µL low-microliter variable-volume pipette in the Accuris NextPette P7700 family, the workhorse pipette for PCR setup, NGS library prep, master mix aliquoting, and other low-microliter pipetting workflows. The 0.5–10 µL range covers the most common molecular-biology bench-pipetting volumes for primer addition, template addition, enzyme aliquoting, and reagent component handling.
Author confidence note (MEDIUM): Same as P7700-1. NextPette brochure is not in the available corpus; specifications confirmed from universe label. Specific calibration accuracy, precision specs, and ergonomic features should be confirmed at first order.
Voltage options: This unit can be ordered with European voltage requirements (230V/50Hz). Contact us to order.
Key Specifications
| SKU | P7700-10 |
| Type | Manual variable-volume pipette |
| Volume range | 0.5 µL to 10 µL (low-microliter) |
| Family position | Second-smallest in the NextPette P7700 family (above the 0.1–1.0 µL P7700-1) |
| Workflow context | Low-microliter pipetting / PCR setup, NGS library prep, master mix aliquoting |
| List price | $283 |
Why the 0.5–10 µL Range Is the Workhorse
The 0.5–10 µL pipette range covers the highest-density per-protocol pipetting volume range in molecular-biology workflows:
| Workflow | Typical 0.5–10 µL pipetting steps |
| PCR setup (25 µL reaction) | Primer (1 µL each), template (1–5 µL), enzyme master mix (12.5 µL; at top of P7700-10 range) |
| qPCR setup (10–25 µL reaction) | Probe (0.5–1 µL), primer (1 µL each), template (1–5 µL) |
| NGS library prep | Adapter (1–2.5 µL), barcode (1–2.5 µL), enzyme aliquots (variable) |
| Restriction digest (20–50 µL reaction) | Enzyme (0.5–2 µL), buffer (2–5 µL), template (variable) |
| Sequencing reactions | Sequencing primer (1 µL), template (1–8 µL) |
| Cloning ligation | T4 ligase (1 µL), buffer (2 µL), insert / vector (2–8 µL) |
A typical molecular-biology bench setup runs 5–10× more 0.5–10 µL pipetting steps per day than any other volume range, making the P7700-10 the most-used pipette in many lab equipping plans.
NextPette Volume-Range Family
| SKU | Volume range | List price |
| P7700-1 | 0.1 – 1.0 µL | $311 |
| P7700-10 (this product) | 0.5 – 10 µL | $283 |
| P7700-20 | 2 – 20 µL | (sister SKU) |
| P7700-100 | 10 – 100 µL | $283 |
| P7700-1000 | 100 – 1,000 µL | (sister SKU) |
| P7700-10M | 1 – 10 mL | (macro-volume) |
Application Profile
PCR / qPCR setup workflows. The workhorse application. Primer addition, template addition, enzyme aliquoting at the 0.5–10 µL range.
NGS library prep facilities. Library construction workflows with adapter / barcode / enzyme handling at the 0.5–10 µL range.
Sanger sequencing reactions. Sequencing primer and template addition at low-microliter volumes.
Restriction-digest and cloning workflows. Enzyme aliquoting and template addition.
Educational laboratory PCR exercises. Molecular-biology coursework PCR setup teaches students standard low-microliter pipetting at this range.
Multi-pipette laboratory equipping. Labs equipping new bench setups consider the P7700-10 the highest-priority single-pipette purchase if only one variable-volume pipette is initially budgeted.
Frequently Asked Questions
What is the volume range of the P7700-10? 0.5 µL to 10 µL, the low-microliter range, second-smallest in the NextPette P7700 family (above the 0.1–1.0 µL P7700-1).
What workflows is the P7700-10 used for? PCR and qPCR setup, NGS library prep, master mix aliquoting, restriction-digest and cloning steps, the highest-density pipetting range in molecular-biology workflows.
Which pipette tips does the P7700-10 use? Typically 10 µL low-retention tips; confirm tip-format compatibility at order.
How does the P7700-10 fit into a full pipette set? It is typically the first-priority variable-volume purchase for a new bench (highest utilization), followed by the P7700-100, P7700-1000, then P7700-1 and P7700-20.
What is the list price of the P7700-10? $283.
Ordering & Configuration
Pipette tip pairing. The P7700-10 typically uses 10 µL low-retention pipette tips; confirm tip-format compatibility at order. Tip procurement is operator-supplied per workflow.
Calibration and maintenance. Same calibration program as the rest of the NextPette family, typical 6–12 month calibration interval per ISO 8655. Pair with the AccuCheck pipette calibration system (W3002A-120ACS) for in-lab calibration.
Workflow-priority sequencing in lab equipping. When equipping a new molecular-biology bench, the P7700-10 is typically the first-priority pipette purchase (highest workflow utilization), followed by P7700-100 (mid-volume buffer / sample handling), P7700-1000 (high-volume buffer transfer), and then P7700-1 and P7700-20 to round out the range.
Confirm at quote: workflow PCR / NGS / cloning pipetting needs, full NextPette family equipping plan, tip-format pairing, calibration program, and lead time.
Warranty: Per Accuris standard warranty terms. Confirm at quote.
Accuris NextPette P7700 manual variable-volume pipette family:
- P7700-1: 0.1–1.0 µL (sub-microliter, $311)
- P7700-10 (this product): 0.5–10 µL (low-microliter workhorse, $283)
- P7700-20: 2–20 µL
- P7700-100: 10–100 µL ($283)
- P7700-1000: 100–1,000 µL
- P7700-10M: 1–10 mL (macro-volume)
Pick the P7700-10 as the first-priority pipette purchase for new lab equipping; size the full pipette family to the workflow's volume-range needs.
