The P7700-10 is the 0.5 to 10 µL low-microliter variable-volume pipette in the Accuris NextPette P7700 family — the workhorse pipette for PCR setup, NGS library prep, master mix aliquoting, and other low-microliter pipetting workflows. The 0.5–10 µL range covers the most common molecular-biology bench-pipetting volumes for primer addition, template addition, enzyme aliquoting, and reagent component handling.
Author confidence note (MEDIUM): Same as P7700-1 — NextPette brochure is not in the available corpus; specifications confirmed from universe label. Specific calibration accuracy, precision specs, and ergonomic features should be confirmed at first order.
Voltage options: This unit can be ordered with European voltage requirements (230V/50Hz). Contact us to order.
Key Specifications
| SKU | P7700-10 |
| Type | Manual variable-volume pipette |
| Volume range | 0.5 µL to 10 µL (low-microliter) |
| Family position | Second-smallest in the NextPette P7700 family (above the 0.1–1.0 µL P7700-1) |
| Workflow context | Low-microliter pipetting — PCR setup, NGS library prep, master mix aliquoting |
| List price | $283 |
Why the 0.5–10 µL Range Is the Workhorse
The 0.5–10 µL pipette range covers the highest-density per-protocol pipetting volume range in molecular-biology workflows:
| Workflow | Typical 0.5–10 µL pipetting steps |
| PCR setup (25 µL reaction) | Primer (1 µL each), template (1–5 µL), enzyme master mix (12.5 µL — at top of P7700-10 range) |
| qPCR setup (10–25 µL reaction) | Probe (0.5–1 µL), primer (1 µL each), template (1–5 µL) |
| NGS library prep | Adapter (1–2.5 µL), barcode (1–2.5 µL), enzyme aliquots (variable) |
| Restriction digest (20–50 µL reaction) | Enzyme (0.5–2 µL), buffer (2–5 µL), template (variable) |
| Sequencing reactions | Sequencing primer (1 µL), template (1–8 µL) |
| Cloning ligation | T4 ligase (1 µL), buffer (2 µL), insert / vector (2–8 µL) |
A typical molecular-biology bench setup runs 5–10× more 0.5–10 µL pipetting steps per day than any other volume range — making the P7700-10 the most-used pipette in many lab equipping plans.
NextPette Volume-Range Family
| SKU | Volume range | List price |
| P7700-1 | 0.1 – 1.0 µL | $311 |
| P7700-10 (this product) | 0.5 – 10 µL | $283 |
| P7700-20 | 2 – 20 µL | (sister SKU) |
| P7700-100 | 10 – 100 µL | $283 |
| P7700-1000 | 100 – 1,000 µL | (sister SKU) |
| P7700-10M | 1 – 10 mL | (macro-volume) |
Application Profile
PCR / qPCR setup workflows — the workhorse application. Primer addition, template addition, enzyme aliquoting at the 0.5–10 µL range.
NGS library prep facilities — library construction workflows with adapter / barcode / enzyme handling at the 0.5–10 µL range.
Sanger sequencing reactions — sequencing primer and template addition at low-microliter volumes.
Restriction-digest and cloning workflows — enzyme aliquoting and template addition.
Educational laboratory PCR exercises — molecular-biology coursework PCR setup teaches students standard low-microliter pipetting at this range.
Multi-pipette laboratory equipping — labs equipping new bench setups consider the P7700-10 the highest-priority single-pipette purchase if only one variable-volume pipette is initially budgeted.
Frequently Asked Questions
What is the volume range of the P7700-10? 0.5 µL to 10 µL — the low-microliter range, second-smallest in the NextPette P7700 family (above the 0.1–1.0 µL P7700-1).
What workflows is the P7700-10 used for? PCR and qPCR setup, NGS library prep, master mix aliquoting, restriction-digest and cloning steps — the highest-density pipetting range in molecular-biology workflows.
Which pipette tips does the P7700-10 use? Typically 10 µL low-retention tips; confirm tip-format compatibility at order.
How does the P7700-10 fit into a full pipette set? It is typically the first-priority variable-volume purchase for a new bench (highest utilization), followed by the P7700-100, P7700-1000, then P7700-1 and P7700-20.
What is the list price of the P7700-10? $283.
Ordering & Configuration
Pipette tip pairing. The P7700-10 typically uses 10 µL low-retention pipette tips; confirm tip-format compatibility at order. Tip procurement is operator-supplied per workflow.
Calibration and maintenance. Same calibration program as the rest of the NextPette family — typical 6–12 month calibration interval per ISO 8655. Pair with the AccuCheck pipette calibration system (W3002A-120ACS) for in-lab calibration.
Workflow-priority sequencing in lab equipping. When equipping a new molecular-biology bench, the P7700-10 is typically the first-priority pipette purchase (highest workflow utilization), followed by P7700-100 (mid-volume buffer / sample handling), P7700-1000 (high-volume buffer transfer), and then P7700-1 and P7700-20 to round out the range.
Confirm at quote: workflow PCR / NGS / cloning pipetting needs, full NextPette family equipping plan, tip-format pairing, calibration program, and lead time.
Warranty: Per Accuris standard warranty terms — confirm at quote.
Accuris NextPette P7700 manual variable-volume pipette family:
- P7700-1 — 0.1–1.0 µL (sub-microliter, $311)
- P7700-10 (this product) — 0.5–10 µL (low-microliter workhorse, $283)
- P7700-20 — 2–20 µL
- P7700-100 — 10–100 µL ($283)
- P7700-1000 — 100–1,000 µL
- P7700-10M — 1–10 mL (macro-volume)
Pick the P7700-10 as the first-priority pipette purchase for new lab equipping; size the full pipette family to the workflow's volume-range needs.
