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The PR4005-100 is the entry pack of the Accuris SmartCheck 50 bp DNA Ladder — sold as 500 µL / 100 lanes (5 µL per lane standard loading volume). Per the Accuris Life Science Reagents Catalog, the SmartCheck DNA Ladders are ready-to-use formulations including loading buffer & tracking dye and are purified by multistep chromatography to produce clear, distinct bands with increased intensity for easy size determination.
| SKU | PR4005-100 |
| Type | DNA Ladder — 50 bp resolution |
| Pack quantity | 500 µL / 100 lanes (5 µL per lane standard loading) |
| Concentration | 0.1 mg/mL |
| Format | Ready-to-use — includes loading buffer & tracking dye |
| Range | 50 bp resolution — fine-resolution DNA sizing for short-amplicon and small-fragment workflows |
| Sister SKUs (same range) | PR4005-500 (500 lanes, $194) |
| Sister SKUs (different range) | PR4010-100 / -500 (100 bp range), PR4100-100 / -500 (1 kb range) |
| List price | $43 |
| SKU family | bp range | Best for |
| PR4005 (this product family) | 50 bp | Short-amplicon PCR (50–500 bp); fine-resolution genotyping; STR analysis |
| PR4010 | 100 bp | Standard PCR products (100 bp – 1 kb); routine genotyping; restriction-digest analysis |
| PR4100 | 1 kb | Larger fragments (500 bp – 10 kb+); plasmid analysis; large-amplicon PCR |
Pick the 50 bp ladder when: the workflow's PCR products or fragments are in the 50–500 bp range and require fine-resolution sizing.
Per Accuris Life Science Reagents Catalog, SmartCheck DNA Ladders feature:
| SKU | Lanes | List price | Per-lane cost |
| PR4005-100 (this product) | 100 | $43 | $0.43/lane |
| PR4005-500 | 500 | $194 | $0.39/lane |
Pick the 100-lane entry pack when: workflow throughput is light (< 100 gel lanes per quarter) — e.g., method-development workflows, small-research-group PCR-product screening, teaching-lab demonstrations. Step to 500-lane pack for production-scale gel screening with meaningful per-lane cost saving.
Short-amplicon PCR-product sizing — the workhorse application. PCR-product confirmation gels for 50–500 bp amplicons.
Fine-resolution genotyping — STR (short tandem repeat) genotyping where 50 bp resolution distinguishes allelic variants.
Restriction-fragment analysis — small-fragment patterns from restriction digests.
Method-development gel runs — protocol-development workflows where gel-based confirmation supports primer design optimization.
Educational laboratory demonstrations — molecular-biology coursework introducing DNA-ladder use and gel-based fragment sizing.
Clinical-research diagnostic gel verification — clinical-research labs running PCR-based diagnostic assays with gel-band size verification.
Per-lane workflow consumable. Each gel lane uses 5 µL of ladder. The 500 µL pack supports 100 lanes before reorder.
Pair with green-light-compatible stain for blue-light visualization (SmartGlow / SYBR Green / Gel Green) or with the SmartGlow PS / LD product line for Accuris-paired workflow.
Storage. Ready-to-use ladder; standard reagent storage applies — typically -20 °C; protect from light if loading-buffer dye is light-sensitive.
Confirm at quote: workflow throughput (drives 100-lane vs 500-lane pack selection), gel-stain chemistry compatibility, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris SmartCheck DNA Ladder family — 50 bp resolution:
- PR4005-100 (this product) — 50 bp ladder, 100 lanes ($43, $0.43/lane)
- PR4005-500 — 50 bp ladder, 500 lanes ($194, $0.39/lane)
Pick the 100-lane entry pack for light workflow; step to 500-lane for production-scale gel screening. Sister ranges PR4010 (100 bp) and PR4100 (1 kb) cover larger-fragment workflows.
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