The EB1000 is the TAE (Tris-acetate-EDTA) buffer powder in the Accuris QuickSilver Powdered Buffer Pack family — sold as 100 pre-measured pouches, each making 1 L of 1× working buffer when dissolved in water. TAE is the DNA agarose-gel-electrophoresis buffer of choice for workflows that recover DNA from gels for downstream enzymatic processing (cloning, restriction digestion, ligation), where the buffer's lower ionic strength supports better DNA recovery vs higher-ionic-strength alternatives like TBE. Per brochure: *"TAE is the buffer of choice when recovering DNA from gels and when downstream processing involves enzymatic reactions."*
Key Specifications
| SKU | EB1000 |
| Type | TAE (Tris-acetate-EDTA) buffer powder, pre-measured pouches |
| Pack quantity | 100 pouches per box |
| Per-pouch yield | 1 L of 1× working buffer (each pouch = 1 L) |
| 1× formulation | 40 mM Tris, 20 mM acetic acid, 1 mM EDTA |
| Workflow application | DNA agarose-gel electrophoresis — both running buffer and gel-preparation buffer |
| Best fit | Workflows recovering DNA from gels for downstream enzymatic processing (cloning, restriction digestion, ligation) |
| Quality / handling | Nuclease-free, protease-free, shelf-stable per brochure; preprinted ID labels included for bottle labeling |
| List price | $285 (100 pouches) |
QuickSilver Workflow — Why Powdered Buffer Pouches
Traditional buffer preparation in the lab:
- Weigh out individual buffer components (Tris, acetic acid, EDTA)
- Dissolve in DI water
- Adjust pH with acid / base
- Verify concentration / pH
- Aliquot for storage; manage stock-solution dilution and re-pH-adjustment for working concentrations
QuickSilver eliminates steps 1, 3, 4, and 5:
- Each pouch contains exactly the right amount of pre-measured powder for 1 L of 1× buffer
- No pH adjustment — formulation is balanced to deliver the correct pH on dissolution
- No weighing — eliminates per-component weighing and weighing-error variance
- No stock-dilution — each pouch makes 1 L of 1× working concentration directly
- Shelf-stable — unlike stock solutions, no evaporation or component crystallization
Workflow time savings. A traditional 1 L TAE buffer preparation takes 10–15 minutes (weigh, dissolve, pH adjust, verify); a QuickSilver pouch takes ~2 minutes (open pouch, empty into water, stir until dissolved).
TAE vs Other Electrophoresis Buffers
| Buffer | Use case | QuickSilver SKU |
| TAE (this product) | DNA agarose gels, especially when DNA recovery for downstream enzymatic processing is needed | EB1000 |
| TBE (Tris-borate-EDTA) | DNA agarose gels, alternative when sharper-band resolution is preferred and downstream enzymatic recovery is not required | (see Accuris reagents catalog for TBE format if available) |
| Tris-Glycine-SDS | SDS-PAGE protein electrophoresis (most common protein running buffer) | EB1100 |
| Tris-MES-SDS | SDS-PAGE for small-to-medium proteins (faster runs than MOPS) | EB1101 |
| Tris-MOPS-SDS | SDS-PAGE for medium-to-large proteins (slower runs than MES) | EB1102 |
Pick TAE (EB1000) when: the workflow recovers DNA from agarose gels for downstream cloning / digestion / ligation. The TAE buffer's lower ionic strength supports cleaner DNA recovery than TBE.
Application Profile
DNA agarose-gel electrophoresis — the workhorse application for TAE; both gel-preparation buffer and running buffer in agarose-gel workflows.
DNA-band excision and recovery for downstream cloning — TAE is the buffer of choice when downstream workflow involves restriction digestion, ligation, or PCR-amplicon recovery from agarose gels.
Restriction-digest verification gels — clone-screening gels where the recovered DNA may be used in downstream verification steps benefit from TAE's enzyme-friendly chemistry.
Routine PCR-product gels — daily PCR-product screening workflows benefit from the rapid QuickSilver buffer-prep workflow.
Teaching laboratory consumable — undergraduate and graduate molecular-biology programs benefit from the simplified buffer-prep workflow (eliminates pH-adjustment training overhead, reduces per-prep error variance for student-cohort exercises).
Field-deployment workflows — environmental sampling, agricultural diagnostics, veterinary diagnostics labs benefit from the shelf-stable, no-equipment-required buffer prep (no pH meter required at point of preparation).
Ordering & Configuration
Per-pouch workflow consumable. Each pouch makes 1 L of 1× buffer; pack of 100 pouches supports 100 L of buffer prep before reorder. Plan procurement to match the workflow's per-month or per-quarter buffer-volume consumption.
Pre-printed ID labels included. Each pack ships with preprinted bottle labels for buffer bottles — no separate labeling-supplies purchase required.
Confirm at quote: workflow buffer-volume consumption, buffer chemistry needed (TAE vs Tris-Glycine-SDS vs PBS / TBS — order the right SKU), shelf-storage capacity, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote. Lot-traceability and shelf-life information per Accuris product-insert documentation.
Accuris QuickSilver buffer-powder family — electrophoresis:
- EB1000 (this product) — TAE buffer (DNA agarose, 100 pouches)
- EB1100 — Tris-Glycine-SDS (SDS-PAGE, 50 pouches)
- EB1102 — Tris-MOPS-SDS (SDS-PAGE for larger proteins, 50 pouches)
See EB1200 / EB1201 / EB1202 / EB1203 for Western-blot / ELISA / cell-culture buffers (PBS, PBS-T, TBS, TBS-T) in the same QuickSilver family.
