The EB1201 is the PBS-T (phosphate-buffered saline with Tween-20) buffer powder in the Accuris QuickSilver Powdered Buffer Pack family — sold as 100 pre-measured pouches, each making 1 L of 1× working buffer when dissolved in water. PBS-T is the standard ELISA / immunoassay wash buffer — the Tween-20 detergent supplements the base PBS chemistry to reduce non-specific antibody binding to plate surfaces during the multi-wash steps of ELISA workflows.
Per brochure: *"PBST is a wash buffer and diluent for ELISA. PBST is supplemented with 0.05% Tween-20."* The base PBS formulation is identical to EB1200 (137 mM NaCl, 2.7 mM KCl, 10 mM phosphate) — the only difference is the addition of 0.05 % Tween-20.
Key Specifications
| SKU | EB1201 |
| Type | PBS-T (PBS + 0.05 % Tween-20) buffer powder, pre-measured pouches |
| Pack quantity | 100 pouches per box |
| Per-pouch yield | 1 L of 1× working buffer (each pouch = 1 L) |
| 1× formulation | 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate, 0.05 % Tween-20 |
| Workflow application | ELISA wash buffer, immunoassay wash steps, primary / secondary antibody dilution buffer for ELISA |
| Sister buffer | EB1200 (PBS without Tween-20 — same $181 price) |
| Quality / handling | Nuclease-free, protease-free, shelf-stable; preprinted ID labels included |
| List price | $181 (100 pouches) |
Why Tween-20 in ELISA Wash Buffer
ELISA workflows involve multiple wash steps between protocol stages (after coating, after blocking, after primary antibody, after secondary antibody, after substrate addition for some protocols). Each wash step removes unbound antibody / antigen / substrate, but non-specific binding of antibodies to the polystyrene plate surface (or to non-target proteins) introduces background signal that degrades assay sensitivity.
Tween-20 (polysorbate 20) is a non-ionic detergent at 0.05 % concentration that:
- Disrupts hydrophobic interactions between antibodies and the plate surface
- Reduces non-specific antibody adsorption during the wash steps
- Maintains specific antibody-antigen binding (the 0.05 % concentration is gentle enough to preserve specific interactions)
- Improves signal-to-background ratio in the final ELISA readout
The 0.05 % concentration is the standard ELISA-optimized concentration — high enough to disrupt non-specific binding, low enough to preserve specific binding. Per-pouch pre-measured Tween-20 ensures consistent concentration across batches (vs make-your-own PBS-T where Tween-20 viscosity makes accurate per-prep dispensing tricky).
ELISA Workflow — Per-Step Buffer Volumes
Typical 96-well ELISA workflow buffer consumption:
| Protocol step | PBS-T volume per plate |
| Wash after coating | 3 × 200 µL × 96 wells = 57.6 mL |
| Wash after blocking | 3 × 200 µL × 96 wells = 57.6 mL |
| Wash after primary antibody | 3–5 × 200 µL × 96 wells = 57.6–96 mL |
| Wash after secondary antibody | 3–5 × 200 µL × 96 wells = 57.6–96 mL |
| Total per plate | ~230–310 mL PBS-T |
A pack of 100 pouches (100 L) supports ~320–435 ELISA plates before reorder — substantial throughput for most clinical-research labs.
EB1201 vs EB1200 — Pick the Right Buffer
| Buffer | Use case |
| EB1201 PBS-T (this product) | ELISA wash buffer, immunoassay wash buffer, antibody dilution for ELISA |
| EB1200 PBS | Cell-culture wash, HPLC mobile phase, MS sample buffer, general physiological buffer |
Pick PBS-T (EB1201) when: the workflow includes ELISA / immunoassay wash steps where Tween-20 detergent matters for assay performance.
Pick PBS (EB1200) when: the workflow does not need detergent (cell culture, HPLC, MS, antibody dilution for IHC / IF rather than ELISA).
Application Profile
ELISA wash workflows — the workhorse application; PBS-T is the standard wash buffer for sandwich ELISA, indirect ELISA, competitive ELISA, and direct ELISA workflows.
Immunoassay wash buffer — Western-blot membrane wash workflows (PBS-T as a Western-wash alternative to TBS-T); flow-cytometry wash where Tween-20 reduces non-specific binding.
Antibody dilution for ELISA — primary and secondary antibody dilutions in ELISA workflows often use PBS-T as the diluent (Tween-20 reduces non-specific binding during the antibody-incubation step).
Microplate-washer workflows — automated microplate washers (Accuris MW9600 SmartWasher 96 or compatible) use PBS-T as the standard wash buffer reservoir charge.
Multi-investigator clinical-research labs — shared ELISA equipment in clinical-research and diagnostic-development labs benefits from per-pouch fresh PBS-T prep (no shared-stock-solution Tween-20-precipitation drift between investigators).
Educational laboratory ELISA exercises — undergraduate / graduate immunology coursework requires PBS-T for student-cohort ELISA exercises; pre-measured pouches simplify per-class buffer prep.
Ordering & Configuration
Per-pouch workflow consumable. Each pouch makes 1 L of 1× buffer; pack of 100 pouches supports ~320–435 96-well ELISA plates.
Pre-printed ID labels included. Each pack ships with preprinted bottle labels for buffer bottles.
Confirm at quote: workflow buffer-volume consumption (ELISA throughput drives pack-quantity needs), PBS-vs-PBS-T selection, microplate-washer pairing (MW9600 / other), shelf-storage capacity, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris QuickSilver ELISA / cell-culture / Western-blot buffer family:
- EB1200 — PBS (general physiological, 100 pouches, $181)
- EB1201 (this product) — PBS-T (ELISA wash, 100 pouches, $181)
- EB1202 — TBS (Western-blot Tris-buffered saline, 100 pouches, $254)
- EB1203 — TBS-T (Western-blot wash, 100 pouches, $254)
Pick PBS-T for ELISA wash workflows; pair with EB1200 PBS for non-detergent steps in the same workflow.
