Accuris qMAX Probe One-Step RT-qPCR Kit, High ROX, 100 Reactions

The PR2121-H-100 is the High ROX formulation of the Accuris qMAX Probe One-Step RT-qPCR Kit — sold as 100-reaction entry pack. The probe-based sister chemistry to the PR2120 qMAX Green One-Step RT-qPCR family — combines reverse transcription (RT) + sequence-specific probe-based qPCR in a single-tube workflow for RNA → quantitative-DNA-readout in one tube with TaqMan / Scorpion / molecular beacon hydrolysis-probe detection. The High ROX formulation is for ABI 7300 / 7500 Standard / 7700 / 7900 cyclers.

Key Specifications

Probe-Based vs Intercalating-Dye One-Step RT-qPCR Decision

The Accuris qMAX One-Step RT-qPCR family includes two parallel chemistry options:

Pick the qMAX Probe One-Step (PR2121) chemistry when:

• The workflow needs sequence-specific detection beyond what primer-pair specificity alone provides
• Pathogen ID workflows where primer-pair non-specifics would compromise calls
• Clinical-research diagnostic workflows where probe-based specificity is the standard
• Multi-target RT-qPCR multiplex (step to PR2122 for explicit multiplex chemistry)

Pick the qMAX Green One-Step (PR2120) chemistry when:

• Workflow is single-target gene expression with well-validated primers
• Probe-cost is prohibitive
• HRM (high-resolution melt) downstream analysis is part of the workflow

qMAX Probe RT-qPCR Multiplex Family

For multi-target probe-based RT-qPCR workflows, step to:

ROX Formulation Selection

Same as PR2120 / PR2000 / PR2001 family — three ROX-level formulations:

Pack-Size Family

Application Profile

Sequence-specific RT-qPCR for clinical-research diagnostics — pathogen RT-qPCR, viral-load quantification, mutation-detection assays where probe-based specificity is the standard.

Viral RNA quantification (research-use) — pathogen-detection workflows where one-step probe chemistry combines workflow simplification + sequence-specificity.

Single-target gene expression with reference-gene-aware design — workflows running probe-based detection for a single target (multiplex with reference gene = step to PR2122).

Method-development RT-qPCR — protocol-development workflows where probe-based detection is optimized.

Multi-investigator clinical-research labs — facilities running standardized probe-based RT-qPCR protocols benefit from the One-Step format's reduced contamination risk vs Two-Step.

Ordering & Configuration

Per-reaction workflow consumable. Each One-Step RT-qPCR reaction consumes proportional reagent.

Probe inventory. Operator supplies sequence-specific probe (TaqMan / Scorpion / molecular beacon — typically purchased separately from probe-synthesis vendors per assay design).

Cycler-compatibility confirmation. High ROX is for Standard-block ABI 7000-series cyclers.

Storage. -20 °C, protect from light. RNase-free handling required throughout.

Pair with RNA template + sequence-specific primers + probe. The kit includes the polymerase + RT + buffer + dNTPs + RNase inhibitor + ROX; operator supplies RNA template, primer pair, and labeled probe.

Confirm at quote: cycler ROX requirement, workflow throughput, evaluation interest (PR2121-H-S sample), one-step vs two-step decision, multiplex panel design (vs PR2122 / PR2124 if multi-target needed), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR2121 qMAX Probe One-Step RT-qPCR Kit family — High ROX:

- PR2121-H-S — Sample, 10 reactions ($14)

- PR2121-H-100 (this product) — 100 reactions ($189, $1.89/rxn)

- PR2121-H-500 — 500 reactions ($821, $1.64/rxn)

- PR2121-H-1000 — 1000 reactions ($1,506, $1.51/rxn)

Pick the qMAX Probe One-Step for sequence-specific RT-qPCR / clinical-research diagnostic / viral RNA quantification; sister chemistry PR2120 (Green) covers SYBR-class workflows; sister chemistries PR2122 / PR2124 cover multi-target multiplex.