The PR2124-N-S is the trial / sample-size SKU of the Accuris qMAX Probe 4× Multiplex No ROX One-Step RT-qPCR Kit family — 20-reaction pack for first-use evaluation of the 4-target multiplex chemistry on Bio-Rad CFX / Roche / Stratagene Mx / Eppendorf / Qiagen 4-fluorophore-channel cyclers.
For complete chemistry rationale (4-target multiplex, 8-primer + 4-probe simultaneous amplification, 4-fluorophore-channel cycler requirement), see the PR2124-N-100 parent page.
Key Specifications
| SKU | PR2124-N-S |
| Type | Sample / trial pack — qMAX Probe 4× Multiplex One-Step RT-qPCR Kit, No ROX |
| Pack quantity | 20 reactions |
| Multiplex capacity | 4 targets per reaction |
| ROX level | No ROX — Bio-Rad CFX, Roche, Stratagene Mx, Eppendorf, Qiagen |
| Cycler requirement | 4-fluorophore-channel cycler |
| Per-reaction cost (sample) | $1.05 (same as PR2122-N-S 2–3 target multiplex sample) |
| Sister chemistry samples | PR2122-N-S (2–3 target multiplex, $21), PR2121-N-S (single-target, $20) |
| Production-scale follow-on | PR2124-N-100 ($196), PR2124-N-500 ($854), PR2124-N-1000 / -2000 / -100BLK / -50BLK (already authored) |
| List price | $21 |
What 20 Reactions Supports — 4× Multiplex Validation Plan
A typical 20-reaction validation plan for the PR2124 4× multiplex sample:
| Validation step | Reactions used |
| No-template control (NTC) | 1 |
| Single-target positive control per probe (4 probes × 1 control each) | 4 |
| 2-target multiplex (each pair) | 6 |
| 3-target multiplex | 3 |
| Full 4-target multiplex | 3 |
| Reserve for re-test | 3 |
| Total | 20 |
This plan supports a comprehensive 4-target multiplex validation: confirm individual probe performance + pairwise multiplex efficiency + full 4-target multiplex amplification — the validation depth needed for confident bulk procurement.
Multiplex Chemistry Comparison Strategy
Labs evaluating the full Accuris multiplex RT-qPCR family can order three samples for direct comparison:
| Sample SKU | Multiplex capacity | Per-rxn cost | Total |
| PR2121-N-S | Single-target | $2.02 | $20 |
| PR2122-N-S | 2–3 target multiplex | $1.05 | $21 |
| PR2124-N-S (this product) | 4 target multiplex | $1.05 | $21 |
| Three-sample evaluation total | | | $62 |
Supports an informed multiplex-capacity-selection decision for the lab's specific panel design.
Application Profile
See PR2124-N-100 parent page for the full application profile (4-target pathogen detection panels, gene expression with 3-reference normalization, allelic discrimination 4-target panels, viral subtyping, cancer-marker multiplex).
Ordering & Configuration
Single-use sample SKU. 20-reaction pack supports a meaningful 4-target multiplex panel validation run.
Probe inventory. Operator supplies 4 sequence-specific probes per panel (each with different fluorophore label per target).
Cycler-compatibility confirmation. No ROX matches non-ABI cyclers AND at least 4 active fluorophore channels required.
Storage. -20 °C, RNase-free handling, protect from light.
Confirm at quote: cycler model + channel count, 4-target multiplex panel design, evaluation intent, and lead time.
Warranty: Per Accuris standard reagent terms — confirm at quote.
Accuris qMAX Probe Multiplex RT-qPCR sample comparison ($62 total for full multiplex evaluation):
- PR2121-N-S — Single-target ($20)
- PR2122-N-S — 2–3 target multiplex ($21)
- PR2124-N-S (this product) — 4-target multiplex ($21)
Pick the 4× multiplex sample for 4-target panel evaluation; sister samples cover single-target and 2–3 target multiplex chemistries.
