Accuris qMAX Probe One-Step 4× Multiplex RT-qPCR Kit, No ROX, 100 Reactions

The PR2124-N-100 is the No ROX formulation of the Accuris qMAX Probe One-Step 4× Multiplex RT-qPCR Kit — sold as 100-reaction entry pack. The highest-multiplex variant in the qMAX RT-qPCR family — engineered for 4-target multiplex (vs PR2122's 2–3 target capacity), supporting 4-fluorophore-channel detection in a single reaction. Same probe-based hydrolysis-probe / molecular-beacon detection chemistry as the PR2121 single-target and PR2122 2–3 target multiplex sister chemistries, but with master-mix formulation engineered for the higher-target-count multiplex efficiency requirements.

Key Specifications

qMAX Multiplex RT-qPCR Family Architecture

The Accuris qMAX One-Step Multiplex RT-qPCR family scales across multiplex capacity:

Pricing observation: PR2124 4× multiplex carries the same per-reaction price as PR2122 2–3 target multiplex ($196 at 100-rxn pack) — Accuris does NOT charge a per-target-count multiplex premium beyond the standard multiplex chemistry tier.

Why a Dedicated 4× Multiplex Master Mix

Standard 2-target multiplex master mixes can support 4-target multiplex with reasonable performance, but 4-target multiplex efficiency requires master-mix optimization:

• Higher polymerase concentration to support 4-target amplification within standard cycle counts
• Buffer composition optimized for 8-primer + 4-probe interaction (4 primer pairs = 8 oligos plus 4 probes simultaneously)
• dNTP balance matched to 4-target amplification stoichiometry
• Hot Start chemistry quality that prevents primer-dimer artifacts when 8 primers are present
• Engineered probe-fluorophore-channel non-interference — 4 fluorophores must report independently

The PR2124 master mix is engineered for these 4-target multiplex requirements.

4-Fluorophore-Channel Cycler Requirement

Critical: 4× multiplex requires a 4-fluorophore-channel qPCR cycler:

Pre-procurement check: Confirm cycler has at least 4 active fluorophore channels (with the 5th typically reserved for ROX on cyclers requiring passive reference) before committing to 4× multiplex chemistry.

Pack-Size Family

Application Profile

4-target pathogen detection panels — the workhorse 4× multiplex application. Multi-pathogen panels (e.g., influenza A + influenza B + RSV + internal control), multi-strain typing, multi-marker diagnostic.

Gene expression with 3-reference normalization — workflows running target gene + 3 reference genes (GAPDH, β-actin, 18S rRNA) for high-confidence normalization.

Allelic discrimination 4-target panels — multi-allele probe panels for SNP-rich genotyping.

Viral subtyping panels — multi-target panels distinguishing viral subtypes via probe-based discrimination.

Cancer-marker multiplex panels — clinical-research workflows running 4-target oncogene / suppressor-gene panels.

Method-development for high-multiplex panel design — protocol-development workflows where 4-target amplification efficiency is being optimized.

Ordering & Configuration

Probe inventory. Operator supplies 4 sequence-specific probes (each with different fluorophore label per target).

Cycler-compatibility confirmation. No ROX for non-ABI cyclers AND at least 4 active fluorophore channels required.

Storage. -20 °C, RNase-free handling, protect from light.

Confirm at quote: cycler model + channel count, multiplex panel design (4-target + fluorophore channel assignments), workflow throughput, evaluation interest (PR2124-N-S sample), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR2124 qMAX Probe One-Step 4× Multiplex RT-qPCR No ROX family pack-size ladder:

- PR2124-N-S — sample (universe blank — confirm)

- PR2124-N-100 (this product) — 100 rxn ($196, $1.96/rxn)

- PR2124-N-500 — 500 rxn ($854, $1.71/rxn)

- PR2124-N-1000 / -2000 / -100BLK / -50BLK — production-scale and bulk packs (already authored)

Pick the 4× multiplex variant for 4-target panels; sister chemistry PR2121 (single-target) and PR2122 (2–3 target multiplex) cover lower-multiplex workflows.