The PR2001-N-50BLK is the 5,000-reaction bulk pack of the Accuris qMAX Probe qPCR Master Mix in the No ROX formulation — a probe-based qPCR master mix for DNA template input (no reverse transcriptase included). Distinct from the PR2121 qMAX Probe One-Step RT-qPCR Kit which combines RT + qPCR in one tube for RNA template input — this PR2001 master mix is the qPCR-only variant for workflows where the template is DNA and the detection chemistry is probe-based (TaqMan®, Scorpions®, or molecular beacon probes). Roughly half the price of the One-Step RT-qPCR kit at the same pack size ($2,196 vs $4,350 for PR2121-N-50BLK) reflects the simpler qPCR-only chemistry without the RT enzyme component.
Universe class flag: The universe price list classifies this SKU as accessory, but a $2,196 reagent master-mix pack at 5,000 reactions is clearly consumable. Page authored as consumable; flagging for post-launch class correction.
Key Specifications
| SKU | PR2001-N-50BLK |
| Type | qPCR Master Mix — probe-based detection chemistry, DNA template input (no RT) |
| Reactions per pack | 5,000 reactions (bulk pack: 1 × 50 mL master mix) |
| Detection chemistry | TaqMan®, Scorpions®, and molecular beacon probe-based real-time qPCR |
| Template input | DNA (genomic DNA, plasmid, PCR product, separately-prepared cDNA) |
| Reverse transcriptase | NOT included (RT-qPCR workflows use the PR2121 One-Step RT-qPCR kit instead) |
| ROX formulation | No ROX — compatible with cyclers that don't require ROX normalization |
| Polymerase | Accuris Hot Start Taq with antibody-mediated hot-start activation |
| Cycler compatibility (No ROX) | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| Sister product (RT-qPCR variant) | PR2121-N-50BLK (One-Step RT-qPCR with RT enzyme included) |
| Sister product (SYBR Green qPCR-only) | PR2000-N-50BLK (SYBR Green chemistry instead of probe) |
| List price | $2,196 |
qPCR vs RT-qPCR — Why Two Separate Kits
The Accuris qMAX Probe family ships in two formulations for different template inputs:
| Kit | Template input | Includes RT? | Price (5,000 rxn) |
| PR2001 qMAX Probe qPCR (this kit's family) | DNA only | No | $2,196 |
| PR2121 qMAX Probe One-Step RT-qPCR | RNA (one-step RT + qPCR in one tube) | Yes | $4,350 |
Pick the PR2001 qPCR-only kit when the template is DNA and a probe-based qPCR workflow is needed. Pick the PR2121 One-Step RT-qPCR kit when the template is RNA and the convenience of one-step RT + qPCR matters.
Probe vs SYBR Green Detection — qPCR-Only Pair
The Accuris qPCR-only master mix family (no RT enzyme) ships in two chemistries:
| Kit | Detection chemistry | When to pick |
| PR2000 qMAX SYBR Green qPCR | SYBR Green I dye | Cost-effective primary screening with simple primer-based detection; melt-curve analysis confirms specificity |
| PR2001 qMAX Probe qPCR (this kit's family) | TaqMan / probe-based | Specific target detection with low-noise quantification; multiplex with single probe per target |
Pick PR2001 (Probe) when probe-based detection is required for specificity beyond what SYBR Green provides (low-abundance targets, splice-variant discrimination, allele-specific quantification, low-level multiplex).
ROX Variant Family — N / L / H
Same convention as the rest of the qMAX family:
| Suffix | ROX level | Compatible cyclers |
| N (this SKU) | No ROX | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| L | Low ROX | Applied Biosystems QuantStudio (newer), Stratagene Mx3000P/Mx3005P |
| H | High ROX | Applied Biosystems 7000/7300/7500/7900HT (older) |
Application Profile
DNA-template probe-based qPCR workflows — clinical-research and pharma R&D labs running probe-based qPCR on genomic DNA, plasmid DNA, or PCR product templates.
Two-step RT-qPCR with probe detection — labs that perform reverse transcription separately and store cDNA for batch probe-based qPCR analysis.
Genotyping with allele-specific probes — workflows using TaqMan or similar probe-based detection for allele-specific quantification.
Pathogen detection workflows — probe-based DNA detection (bacterial, viral DNA) at scale.
Bulk-pack institutional procurement — labs running >5,000 probe-based qPCR reactions per year benefit from the bulk-pack per-reaction discount.
Ordering & Configuration
Storage & handling. Per kit instructions — typical qPCR master mix storage at −20 °C with limited freeze-thaw cycle tolerance.
Cycler compatibility verification. Critical at order: confirm the lab's real-time cycler platform requires the No ROX formulation.
Probe procurement separately. This kit supplies the master mix; probes (TaqMan, Scorpions, molecular beacon) are operator-supplied per the workflow's specific target.
Confirm at quote: real-time cycler platform and ROX requirement, template type (DNA — confirms PR2001 vs PR2121 RT-qPCR sister kit), expected workflow throughput, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
qMAX Probe qPCR Mix family — pack-size variants for No ROX:
- PR2001-N-50BLK (this product) — 5,000 reactions, bulk pack
- PR2001-N-25BLK — 2,500 reactions, smaller bulk pack
- PR2001-N-1000 — 1,000 reactions
- PR2001-N-500 — 500 reactions
- PR2001-N-100 — 100 reactions
- PR2001-N-S — sample size
For RNA template input requiring one-step RT-qPCR, see the PR2121 qMAX Probe One-Step RT-qPCR Kit sister family. For SYBR Green detection (vs probe), see PR2000 qMAX SYBR Green qPCR Mix.
