The PR2001-N-25BLK is the 2,500-reaction bulk pack of the Accuris qMAX Probe qPCR Master Mix in the No ROX formulation — a probe-based qPCR master mix optimized for TaqMan, Scorpion, and molecular beacon probe detection chemistries on DNA template input (no reverse transcriptase included). Supplied as a single 25 mL bottle. Probe-based sister to the PR2000 qMAX SYBR Green qPCR Mix — same pack format, same bulk economics, probe-based detection chemistry instead of SYBR Green dye-based detection.
Pricing note: The PR2000 PCR Bulk Reagents brochure prints a $1,030 list price for this pack; the 2026 universe price list specifies $1,220. Accuris has updated list pricing between brochure issue and 2026 master price list — this page reflects the current 2026 list price.
Key Specifications
| SKU | PR2001-N-25BLK |
| Type | qPCR Master Mix — probe-based detection chemistry, DNA template input (no RT) |
| Reactions per pack | 2,500 reactions (bulk pack: 1 × 25 mL master mix bottle) |
| Concentration | 2× master mix |
| Detection chemistry | Probe-based — optimized for TaqMan™, Scorpion®, and molecular beacon probes |
| Template input | DNA (genomic, plasmid, PCR product, separately-prepared cDNA) |
| Reverse transcriptase | NOT included (RT-qPCR workflows use the PR2121 One-Step RT-qPCR Probe family) |
| ROX formulation | No ROX — compatible with cyclers that don't require ROX normalization |
| Polymerase | Accuris Hot Start Taq with antibody-mediated hot-start activation |
| Cycler compatibility (No ROX) | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| Larger bulk sister | PR2001-N-50BLK (5,000 reactions / 50 mL — same chemistry, larger pack) |
| Sister product (SYBR Green) | PR2000-N-25BLK (qMAX SYBR Green — dye-based detection) |
| Sister product (RT-qPCR variant) | PR2121-N-25BLK (One-Step RT-qPCR Probe with RT enzyme included) |
| List price | $1,220 |
| Returns | Non-returnable — perishable reagent per Accuris reagent-return policy |
Probe-Based vs SYBR Green Detection — Why Two Master Mix Chemistries
Accuris ships two qPCR master-mix chemistries for different detection-system requirements:
| Master mix | Detection chemistry | When to pick |
| PR2001 qMAX Probe (this product's family) | Probe-based — labeled hydrolysis probe (TaqMan), molecular beacon, or Scorpion probe binds the amplicon and reports signal | Sequence-specific detection (multiplex, allele-specific, low-copy targets, clinical-diagnostic assays where specificity is critical) |
| PR2000 qMAX SYBR Green | Dye-based — SYBR Green I binds double-stranded DNA non-specifically | Single-target qPCR with melt-curve confirmation; cost-effective screening; gene-expression workflows where probe design overhead is unwarranted |
Probe-based is the right pick when the workflow is multiplex (multiple targets in one tube, each with a differently-labeled probe), the assay must distinguish sequence variants or alleles (allele-specific probes), specificity matters more than the cost overhead of probe synthesis, the assay is a clinical-research or diagnostic-style workflow with regulatory specificity expectations, or low-copy / rare-target detection needs probe specificity to reduce false-positive risk.
SYBR Green is the right pick when the workflow is single-target qPCR with melt-curve verification of amplicon identity, for cost-effective screening or large-scale gene-expression workflows, or when probe design overhead is unwarranted for the application.
TaqMan / Scorpion / Molecular Beacon Probe Compatibility
Per the PR2000 PCR Bulk Reagents brochure: the qMAX Probe master mix is optimized for TaqMan™ (hydrolysis probes), Scorpion®, and molecular beacon probe detection chemistries. The buffer composition supports the kinetics of all three probe formats:
- TaqMan (hydrolysis) — 5'-fluorophore / 3'-quencher hydrolysis probe; Taq polymerase 5'→3' exonuclease activity cleaves the probe during extension, separating fluorophore from quencher and increasing fluorescence with each cycle
- Scorpion — bifunctional primer-probe hybrid; the probe portion folds back to a self-quenched conformation that opens during extension to report signal
- Molecular beacon — stem-loop probe with fluorophore / quencher held adjacent in the closed conformation; opens upon hybridization to amplicon, separating fluorophore from quencher
Pick whichever probe format the assay uses; the qMAX Probe master mix supports all three.
ROX Variant Family — N / L / H
Same convention as the qMAX Green family — pick by cycler platform's ROX requirement:
| Suffix | ROX level | Compatible cyclers |
| N (this SKU) | No ROX | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| L | Low ROX | Applied Biosystems QuantStudio (newer), Stratagene Mx3000P / Mx3005P |
| H | High ROX | Applied Biosystems 7000 / 7300 / 7500 / 7900HT (older) |
The 25BLK and 50BLK bulk packs ship in the No ROX (N) formulation per the brochure ordering table; L and H ROX bulk packs may not be available — confirm at quote if the cycler requires Low or High ROX.
qPCR vs RT-qPCR — Why Two Probe-Based Kits
The Accuris probe-based qPCR family ships in two formulations for different template inputs:
| Kit | Template input | Includes RT? | When to pick |
| PR2001 qMAX Probe qPCR (this kit's family) | DNA only | No | DNA-template probe-based qPCR (genomic SNP, copy-number qPCR, MRD-style DNA monitoring) |
| PR2121 qMAX Probe One-Step RT-qPCR | RNA (one-step RT + qPCR in one tube) | Yes | Probe-based RT-qPCR for gene expression, viral RNA, RNA-template diagnostics |
Pick PR2001 for DNA-template probe-based qPCR. Pick PR2121 for one-step RT-qPCR with probe-based detection on RNA template.
Bulk-Pack Format — 25 mL vs 50 mL
| Bulk pack | Format | Reactions | When to pick |
| PR2001-N-25BLK (this product) | 1 × 25 mL bottle | 2,500 reactions | High-throughput workflows where 2,500 reactions matches a quarterly or sub-annual reagent cycle |
| PR2001-N-50BLK | 1 × 50 mL bottle | 5,000 reactions | Highest-throughput workflows — pathogen-detection production, large clinical-research cohorts, multi-bench facilities |
Application Profile
Multiplex pathogen-detection qPCR — multiplex assays detecting multiple pathogen targets in one tube using differently-labeled TaqMan probes.
Allele-specific qPCR / SNP genotyping — TaqMan SNP Assays and similar probe-based allele-discrimination workflows.
Clinical-research probe-based qPCR — workflows where probe-based detection delivers the specificity required for clinical-research applications (where SYBR Green's non-specific dsDNA binding is insufficient).
Cannabis-testing probe-based qPCR — multiplex cannabis-genomics qPCR assays using probe-based detection for sex determination, strain-ID multiplexing, or pathogen testing on cannabis material.
Two-step RT-qPCR with probe-based detection — labs that perform reverse transcription separately and then run probe-based qPCR on the cDNA template.
Bulk-pack institutional procurement — labs running >2,500 probe-based qPCR reactions per quarter benefit from the bulk-pack per-reaction discount.
Ordering & Configuration
Storage & handling. Per kit instructions — typical qPCR master mix storage at −20 °C with limited freeze-thaw cycle tolerance. Single 25 mL bottle benefits from making working aliquots on first thaw.
Cycler compatibility verification. Critical at order: confirm the lab's real-time cycler platform requires the No ROX formulation. If the cycler requires Low or High ROX, confirm bulk-pack variant availability or order non-bulk pack sizes.
Probe procurement. Probes (TaqMan, Scorpion, molecular beacon) are ordered separately — confirm probe inventory and design at order.
Returns. Per the universe price-list category header: reagent products including qPCR mixes are non-returnable due to perishable nature.
Confirm at quote: real-time cycler platform and ROX requirement, probe format and design (TaqMan / Scorpion / molecular beacon), template type (DNA — confirms PR2001 vs PR2121 RT-qPCR sister), expected workflow throughput, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
qMAX Probe qPCR Mix family — bulk and pack-size variants for No ROX:
- PR2001-N-25BLK (this product) — 2,500 reactions, 1 × 25 mL bulk pack
- PR2001-N-50BLK — 5,000 reactions, 1 × 50 mL bulk pack (largest)
- PR2001-N-1000 / PR2001-N-500 / PR2001-N-100 — non-bulk pack sizes
- PR2001-N-S — sample size
For dye-based detection (SYBR Green), see the PR2000 qMAX SYBR Green sister family. For RNA template input requiring one-step probe-based RT-qPCR, see the PR2121 qMAX Probe One-Step RT-qPCR sister family.
