Accuris qMAX Probe qPCR Mix, High ROX, 100 Reactions

The PR2001-H-100 is the High ROX formulation of the Accuris qMAX Probe qPCR Master Mix — sold as 100-reaction entry pack. Per the Accuris Life Science Reagents Catalog: *"qMAX Probe qPCR Mix — Single tube formulation: Ready to use 2X Master Mix (Includes Hot Start Taq DNA Polymerase). Compatible with popular hydrolysis & beacon probes. Optimized buffer for maximum efficiency."* The probe-based detection chemistry is engineered for TaqMan™ hydrolysis probes, Scorpion® probes, and molecular beacon probes — the workhorse chemistry for sequence-specific qPCR detection where SYBR-class intercalating chemistry would be insufficiently specific.

The High ROX (-H) formulation designates compatibility with ABI 7000-series Standard-block cyclers (7300, 7500 Standard, 7700, 7900) that require High ROX passive reference dye normalization.

Key Specifications

Probe-Based vs Intercalating-Dye qPCR Chemistry Decision

The Accuris qMAX qPCR family includes two parallel chemistry options:

Pick the qMAX Probe (PR2001) chemistry when:

• The workflow needs sequence-specific detection beyond what primer-pair specificity alone provides
• Multiplex qPCR with multiple targets in one reaction (each target's probe carries a different fluorophore)
• Pathogen ID workflows where primer-pair non-specifics would compromise calls
• Clinical-research diagnostic workflows where probe-based specificity is the standard

Conversely, pick the qMAX Green (PR2000) intercalating-dye chemistry when the workflow is single-target gene expression with well-validated primers, probe cost is prohibitive, or HRM (high-resolution melt) downstream analysis is part of the workflow.

ROX Formulation Selection — Pick the Right Variant for the Cycler

Same as PR2000 family — three ROX-level formulations matched to qPCR cycler family:

Pack-Size Family

Per Brochure qMAX Probe qPCR Mix Features

Per the Accuris Life Science Reagents Catalog, the qMAX Probe qPCR Mix is a single-tube, ready-to-use 2X master mix that includes Accuris Hot Start Taq DNA Polymerase, dNTPs, and an optimized buffer for maximum efficiency, and is compatible with popular hydrolysis and beacon probes.

Application Profile

Sequence-specific qPCR for clinical-research diagnostics — pathogen detection, viral-load quantification, mutation-detection assays where probe-based specificity is the standard.

Multiplex qPCR — multi-target detection in a single reaction with different fluorophore-labeled probes per target (typical multiplex panels: 2–5 targets).

Gene expression with reference-gene normalization — workflows running target gene + reference gene (e.g., GAPDH, β-actin) probes simultaneously in multiplex.

Genotyping with allele-specific probes — SNP detection workflows using competing probes for each allele.

Pathogen detection workflows — research-use viral / bacterial detection with sequence-specific probes.

Method-development qPCR — protocol-development workflows where probe-based detection is being optimized.

Ordering & Configuration

Per-reaction workflow consumable. Each qPCR reaction uses 12.5 µL of the 2X master mix.

Probe inventory. Operator supplies the sequence-specific probe (TaqMan / Scorpion / molecular beacon — typically purchased separately from probe-synthesis vendors per assay design).

Cycler-compatibility confirmation at order. High ROX is for Standard-block ABI 7000-series cyclers, not Fast-block.

Storage. -20 °C, protect from light to preserve fluorescent dye chemistry.

Confirm at quote: cycler ROX requirement, workflow throughput, multiplex panel design (probe count and fluorophore-channel assignments), evaluation interest (PR2001-H-S sample at $14), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR2001 qMAX Probe qPCR Mix family — ROX variant selection:

- PR2001-N-* — No ROX (Bio-Rad CFX, Roche, Stratagene Mx, etc.)

- PR2001-L-* — Low ROX (ABI ViiA 7, QuantStudio Fast, etc.)

- PR2001-H-* (this product family) — High ROX (ABI 7300 / 7500 Standard / 7700 / 7900)

Pick the High ROX variant for ABI 7000-series Standard-block cyclers; sister chemistry PR2000-H-* covers intercalating-dye SYBR-class workflows.