The PR2124-N-50BLK is the 5,000-reaction bulk pack of the Accuris qMAX Multiplex One-Step 4X RT-qPCR Kit in the No ROX formulation — a 4× concentrated multiplex master-mix variant of the qMAX Multiplex family (sister to the standard 2× PR2122 kit). The 4× concentration delivers two key workflow improvements over the standard PR2122: lower per-reaction master-mix volume (master mix takes a smaller fraction of the total reaction volume, leaving more room for sample template + primers + probes) and higher-multiplex panel support (more probe sets fit in the reaction without diluting the master-mix below working concentration). The N suffix denotes No ROX; 50BLK denotes the 5,000-reaction bulk-discount pack.
Key Specifications
| SKU | PR2124-N-50BLK |
| Type | One-Step RT-qPCR Kit — 4× concentrated multiplex probe-based |
| Reactions per pack | 5,000 reactions (bulk pack: 2 × 25 mL master mix per universe label hint — concentrated 4× formulation) |
| Master mix concentration | 4× concentrated (vs PR2122 sister kit at standard 2× concentration) |
| Detection chemistry | High-multiplex probe-based real-time qPCR |
| Multiplexing capacity | High-multiplex panels — typically 5+ targets per reaction with the concentrated 4× chemistry |
| ROX formulation | No ROX — compatible with cyclers that don't require ROX normalization |
| Workflow | One-step (RNA template → high-multiplex qPCR result in one tube, one reaction setup) |
| Reverse transcriptase | Thermostable MMLV-derived |
| Polymerase | Accuris Hot Start Taq with antibody-mediated hot-start activation |
| RNase inhibitor | Included in optimized buffer |
| Cycler compatibility (No ROX) | Bio-Rad CFX, Roche LightCycler, and similar real-time cyclers that don't require ROX normalization |
| Sister pack size | PR2124-N-100BLK (10,000 reactions, double-throughput bulk pack) |
Why a 4× Concentrated Variant
The standard 2× master-mix formulation occupies half the reaction volume by design (typical 20 µL reaction = 10 µL of 2× master mix + 10 µL of sample/primers/probes/water). For high-multiplex panels (5+ targets), the primers + probes alone may exceed the available 10 µL — requiring either reaction-volume increases (which dilute the cycler's signal-readout volume) or smaller per-component volumes (which compromise primer/probe concentration optimization).
The 4× concentrated formulation allocates only 25% of the reaction volume to master mix (5 µL of 4× master mix in a 20 µL reaction), leaving 75% for sample + multiplex primer/probe sets. This enables high-multiplex panel design without reaction-volume bloat or reagent-concentration compromise.
Multiplex Family Position
| Kit | Concentration | Multiplex capacity | When to pick |
| PR2122 qMAX Multiplex | Standard 2× | 3-5 targets per reaction | Mid-multiplex panels |
| PR2124 qMAX Multiplex 4X (this kit's family) | 4× concentrated | High-multiplex panels (5+ targets) | High-multiplex panel design with reaction-volume constraints |
Pick the PR2124 4X variant when the multiplex panel design requires more than the standard 2× master mix's available reaction-volume allocation supports.
ROX Variant Family — N / L / H
Same convention as the rest of the qMAX family:
| Suffix | ROX level | Compatible cyclers |
| N (this SKU) | No ROX | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| L | Low ROX | Applied Biosystems QuantStudio (newer), Stratagene Mx3000P/Mx3005P |
| H | High ROX | Applied Biosystems 7000/7300/7500/7900HT (older) |
Application Profile
High-multiplex pathogen detection panels — respiratory viral panels with 5-8 targets, sexually transmitted infection panels with multiple pathogen targets, gastrointestinal pathogen panels.
Comprehensive gene-expression panels — pathway-analysis workflows quantifying multiple target genes plus housekeeping genes per reaction.
Allele-specific multiplex — workflows quantifying multiple allele variants in a single reaction with high-multiplex probe sets.
Diagnostic-research-grade high-multiplex RT-qPCR — translational research workflows developing high-multiplex panels for downstream diagnostic applications.
Bulk-pack institutional procurement at high-multiplex scale — labs running >5,000 high-multiplex RT-qPCR reactions per year benefit from the bulk-pack per-reaction discount; for higher throughput, the PR2124-N-100BLK 10,000-reaction sister pack is available.
Ordering & Configuration
Storage & handling. Per kit instructions — typical RT-qPCR master mix storage at −20 °C with limited freeze-thaw cycle tolerance.
Cycler compatibility verification. Critical at order: confirm the lab's real-time cycler platform requires the No ROX formulation AND has sufficient channel capability for the high-multiplex panel design (5+ channel cyclers required for 5+ target panels).
Reaction-volume planning. The 4× concentrated formulation supports more flexible reaction-volume allocation; confirm the lab's standard reaction volume (20 µL or other) and primer/probe concentrations at order to validate the 4× chemistry matches the workflow design.
Probe procurement separately. This kit supplies the master mix; multiplex probe sets are operator-supplied.
Confirm at quote: real-time cycler platform and ROX requirement, multiplex panel design and channel count, expected workflow throughput (5,000-reaction pack vs the 10,000-reaction PR2124-N-100BLK sister), shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
qMAX RT-qPCR family — pack-size and concentration variants:
- PR2124-N-50BLK (this product) — Multiplex 4X, 5,000 reactions
- PR2124-N-100BLK — Multiplex 4X, 10,000 reactions (double-throughput bulk pack)
- PR2122-N-50BLK — Multiplex (standard 2× concentration), 5,000 reactions
- PR2121 — Single-target Probe; PR2120 — SYBR Green
Pick the PR2124 4X concentrated variant for high-multiplex panel design where reaction-volume allocation matters.
