The PR2124-N-100BLK is the 10,000-reaction double-throughput bulk pack of the Accuris qMAX Multiplex One-Step 4× RT-qPCR Kit in the No ROX formulation. Same chemistry as the PR2124-N-50BLK (5,000-reaction sister pack) — 4× concentrated multiplex probe-based one-step RT-qPCR with MMLV reverse transcriptase + Hot Start Taq polymerase + RNase inhibitor, No ROX formulation. The 100BLK pack delivers double the reaction count for higher-throughput institutional workflows where 5,000 reactions per cycle of master-mix purchase is insufficient. Pricing scales linearly at this bulk tier ($10,295 for 100BLK vs $5,437 for 50BLK = exactly 2×, no incremental bulk discount over the already-discounted 50BLK).
Key Specifications
| SKU | PR2124-N-100BLK |
| Type | One-Step RT-qPCR Kit — 4× concentrated multiplex probe-based |
| Reactions per pack | 10,000 reactions (double-throughput bulk pack) |
| Master mix concentration | 4× concentrated (vs PR2122 sister kit at standard 2× concentration) |
| Detection chemistry | High-multiplex probe-based real-time qPCR |
| Multiplexing capacity | High-multiplex panels — typically 5+ targets per reaction |
| ROX formulation | No ROX — compatible with cyclers that don't require ROX normalization |
| Workflow | One-step (RNA template → high-multiplex qPCR result in one tube) |
| Reverse transcriptase | Thermostable MMLV-derived |
| Polymerase | Accuris Hot Start Taq with antibody-mediated hot-start activation |
| RNase inhibitor | Included in optimized buffer |
| Cycler compatibility (No ROX) | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| Sister pack size | PR2124-N-50BLK (5,000 reactions, half-throughput) |
| Pack size context | 100BLK = 10,000-reaction double-bulk pack at $10,295 (exact 2× scale of the 50BLK at $5,437) |
| List price | $10,295 |
When to Order the 100BLK vs 50BLK
The double-throughput 100BLK pack is the right pick when:
- Workflow throughput exceeds 5,000 reactions per master-mix purchase cycle — labs running multiplex RT-qPCR at >5,000 reactions per quarter benefit from the larger pack to reduce reorder frequency.
- Institutional cold-storage capacity supports the larger pack — the 10,000-reaction pack requires more freezer storage volume; confirm the lab's −20 °C cold-storage capacity at order.
- Workflow consistency across the pack lifetime is important — using a single master-mix lot across more reactions reduces lot-to-lot variation in workflow results (relevant for translational research and validation studies).
For lower-throughput workflows or when freezer storage is constrained, the PR2124-N-50BLK sister pack (5,000 reactions) is the right pick.
Pricing note: Pricing scales exactly 2× from 50BLK to 100BLK (no incremental volume discount at this tier). The procurement decision is workflow-throughput-driven and reorder-frequency-driven, not price-driven.
Multiplex Family Position & ROX Variants
See the PR2124-N-50BLK page for the full multiplex family context (PR2120 SYBR vs PR2121 Probe vs PR2122 Multiplex vs PR2124 Multiplex 4X) and ROX variant family (N / L / H).
Workflow & Application
For the complete workflow specifications, multiplex panel design considerations, and application profile, see the PR2124-N-50BLK sister page. The 100BLK pack delivers identical chemistry at double the reaction count.
Application profile (summary):
- Institutional pathogen-detection panels at high-throughput scale
- Comprehensive gene-expression pathway panels
- Allele-specific multiplex at institutional scale
- Diagnostic-research-grade high-multiplex RT-qPCR validation studies
- Multi-site or multi-cohort clinical-research studies that need consistent master-mix lot across all sites/cohorts
Ordering & Configuration
Storage & handling. Per kit instructions — typical RT-qPCR master mix storage at −20 °C with limited freeze-thaw cycle tolerance. The larger 100BLK pack requires more freezer storage volume than the 50BLK; confirm storage capacity at order.
Cycler compatibility verification. Critical at order: confirm the lab's real-time cycler platform requires the No ROX formulation AND has sufficient channel capability for the high-multiplex panel design.
Reorder-frequency planning. The 100BLK pack reduces reorder frequency for high-throughput workflows; pair with a workflow-volume forecast to size the procurement scope correctly.
Confirm at quote: real-time cycler platform and ROX requirement, multiplex panel design and channel count, expected workflow throughput (5,000-reaction PR2124-N-50BLK vs 10,000-reaction this SKU), cold-storage capacity, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
qMAX Multiplex 4X — pack-size variants for No ROX:
- PR2124-N-100BLK (this product) — 10,000 reactions, double-throughput bulk pack
- PR2124-N-50BLK — 5,000 reactions, standard bulk pack
- PR2124-N-500 — 500 reactions
- PR2124-N-100 — 100 reactions
- PR2124-N-S — sample size
Pick the 100BLK for high-throughput institutional workflows requiring single-lot consistency across 10,000 reactions.
