The PR2120-N-50BLK is the 5,000-reaction bulk pack of the Accuris qMAX Green One-Step RT-qPCR Kit in the No ROX formulation — a SYBR Green-based one-step RNA-to-qPCR kit that combines reverse transcription and qPCR amplification in a single tube. The kit's optimized buffer includes a powerful RNase inhibitor and an extremely thermostable MMLV-derived reverse transcriptase for robust first-strand cDNA synthesis, paired with Accuris Hot Start Taq Polymerase using rapid antibody-mediated hot-start activation (allows reaction preparation at room temperature). The No ROX formulation is compatible with cyclers that don't require ROX normalization (Bio-Rad CFX, Roche LightCycler, and similar real-time cyclers). The 50BLK suffix denotes the 5,000-reaction bulk-discount pack — institutional procurement scale at substantial per-reaction discount over smaller pack sizes.
Key Specifications
| SKU | PR2120-N-50BLK |
| Type | One-Step RT-qPCR Kit — SYBR Green detection chemistry |
| Reactions per pack | 5,000 reactions (bulk pack: typically 1 × 50 mL master mix + 4 × 1.25 mL ROX-free buffer per universe label hint) |
| Detection chemistry | SYBR Green I dye-based real-time qPCR |
| ROX formulation | No ROX — compatible with cyclers that don't require ROX normalization |
| Workflow | One-step (RNA template → qPCR result in one tube, one reaction setup) |
| Reverse transcriptase | Thermostable MMLV-derived |
| Polymerase | Accuris Hot Start Taq with antibody-mediated hot-start activation |
| RNase inhibitor | Included in optimized buffer |
| Cycler compatibility (No ROX) | Bio-Rad CFX, Roche LightCycler, and similar real-time cyclers that don't require ROX normalization |
| Pack size context | 50BLK = bulk-discount pack at as low as $0.50 per reaction (per Accuris brochure) |
| List price | $5,153 |
ROX Variant Family — N / L / H
The qMAX Green One-Step RT-qPCR Kit ships in three ROX-formulation variants for different real-time cycler platforms:
| Suffix | ROX level | Compatible cyclers |
| N (this SKU) | No ROX | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| L | Low ROX | Applied Biosystems QuantStudio (newer), Stratagene Mx3000P/Mx3005P |
| H | High ROX | Applied Biosystems 7000/7300/7500/7900HT (older) |
Match the ROX variant to the lab's real-time cycler platform — using the wrong ROX formulation produces inaccurate normalization in cyclers that depend on ROX as a passive reference dye.
SYBR Green vs Probe Detection — qMAX Family Position
The qMAX RT-qPCR family ships in four detection chemistries:
| Kit family | Detection | When to pick |
| PR2120 qMAX Green (this kit's family) | SYBR Green I dye | Cost-effective primary screening, gene-expression analysis with simple primer-based detection |
| PR2121 qMAX Probe | TaqMan / probe-based | Specific target detection, multiplex with single probe per target, low-noise quantification |
| PR2122 qMAX Multiplex | Multiplex probe | Multiple targets per reaction (multiplex panel) |
| PR2124 qMAX Multiplex 4X | Concentrated multiplex | High-multiplex panels, concentrated workflow |
Pick PR2120 (Green) when the workflow uses SYBR-based detection and the cost-per-reaction matters; pick the probe variants for higher specificity or multiplex requirements.
Workflow & Application
One-step RT-qPCR workflow. RNA template + kit master mix + gene-specific primers in a single tube → reverse transcription + qPCR amplification + real-time detection in one thermal cycler run. Reduces pipetting steps and contamination risk vs two-step (separate RT then qPCR) workflows.
SYBR Green detection. SYBR Green I dye binds to dsDNA produced during qPCR amplification, generating fluorescence proportional to product concentration. Cost-effective vs probe-based chemistry; requires melt-curve analysis to confirm specificity (no probe means SYBR detects any dsDNA, including primer-dimer artifacts).
Hot-start polymerase. Antibody-mediated hot-start activation prevents non-specific amplification during reaction setup at room temperature — improves specificity vs non-hot-start Taq.
Cycler compatibility — No ROX. This formulation works with cyclers that don't use ROX as a passive reference dye for normalization. Confirm cycler ROX requirement at order; mismatched ROX formulation produces inaccurate Ct values on cyclers that require ROX normalization.
Application Profile
Routine gene-expression analysis at scale — institutional clinical-research, pharma R&D, and academic core facilities running gene-expression profiling workflows benefit from bulk-pack pricing economics.
Pathogen detection workflows — clinical-research and surveillance labs running RT-qPCR-based pathogen detection (viral RNA quantification, bacterial RNA detection) at scale.
Diagnostic-research-grade RT-qPCR — translational research workflows that require validated RT-qPCR chemistry at institutional volume.
Educational and training labs — molecular biology curricula teaching RT-qPCR methodology benefit from bulk-pack pricing for student-cohort throughput.
Bulk-pack institutional procurement — labs running >5,000 RT-qPCR reactions per year benefit from the bulk-pack per-reaction discount vs purchasing smaller packs.
Ordering & Configuration
Storage & handling. Per kit instructions — typical RT-qPCR master mix storage at −20 °C with limited freeze-thaw cycle tolerance. Confirm storage and handling requirements at receipt per Accuris kit instructions.
Cycler compatibility verification. Critical at order: confirm the lab's real-time cycler platform requires the No ROX formulation. If the cycler requires Low ROX or High ROX, order the PR2120-L-50BLK or PR2120-H-50BLK variant instead.
Confirm at quote: real-time cycler platform and ROX requirement, expected workflow throughput (5,000-reaction bulk pack appropriate for institutional volume; smaller packs for lower-throughput labs), shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
qMAX Green One-Step RT-qPCR Kit family — pack-size variants for No ROX:
- PR2120-N-50BLK (this product) — 5,000 reactions, bulk pack
- PR2120-N-1000 — 1,000 reactions
- PR2120-N-500 — 500 reactions
- PR2120-N-100 — 100 reactions
- PR2120-N-S — sample size
Match the pack size to the lab's expected workflow throughput. The 50BLK bulk pack delivers institutional-scale economics at the lowest per-reaction price point.
