Accuris qMAX Green One-Step RT-qPCR Kit, High ROX, 100 Reactions

The PR2120-H-100 is the High ROX formulation of the Accuris qMAX Green One-Step RT-qPCR Kit — sold as 100-reaction entry pack. The kit combines reverse transcription (RT) + quantitative real-time PCR (qPCR) in a single-tube workflow for RNA → quantitative-DNA-readout in one tube — the workhorse chemistry for gene expression quantification, viral RNA quantification, and other RNA-target qPCR workflows where two-step (separate cDNA synthesis + qPCR) workflow time is a bottleneck.

The High ROX (-H) formulation designates compatibility with ABI 7000-series Standard-block cyclers (7300, 7500 Standard, 7700, 7900) requiring High ROX passive reference dye normalization.

Key Specifications

qMAX One-Step RT-qPCR Family Architecture

The Accuris qMAX One-Step RT-qPCR family includes two parallel chemistry options matched to detection chemistry:

Plus the higher-multiplex chemistry families:

• PR2122 qMAX Probe One-Step Multiplex RT-qPCR — multi-target multiplex with multiple fluorophore-labeled probes
• PR2124 qMAX Probe One-Step Multiplex 4× RT-qPCR — high-multiplex (4-target) workflows

qMAX Green One-Step RT-qPCR vs Two-Step Workflow

Two-step workflow (PR2110 cDNA Flex Kit + PR2000 qMAX Green qPCR Mix):

1. cDNA synthesis (~30 min, separate tube)
2. cDNA aliquoting into qPCR tubes
3. qPCR (~1 hr)
4. Total: ~1.5–2 hr workflow with two-tube transfer + aliquoting

One-step workflow (this kit):

1. Combined RT + qPCR in single tube (~1.5 hr including initial RT incubation + qPCR cycling)
2. Total: ~1.5 hr workflow with no inter-step transfer

Workflow advantages of one-step:

• Reduced contamination risk — closed-tube workflow
• Reduced sample loss — no transfer step
• Faster workflow — single-tube setup
• Better low-copy sensitivity — entire RT product participates in qPCR

Trade-off: Two-step workflow allows multiple downstream qPCR readouts from same cDNA prep (one cDNA → many qPCR reactions for multi-gene expression panel). One-step is single-target per reaction — for multi-target panels, the kit is consumed per target × per sample.

ROX Formulation Selection

Same as PR2000 / PR2001 family — three ROX-level formulations matched to qPCR cycler family:

PR2120-H Pack-Size Family

Per-reaction cost note: The 100-rxn pack at $1.89/rxn is significantly higher than the 500-rxn pack at $0.73/rxn — reflects the fixed cost of the multi-component kit format distributed across pack size. The 100-rxn pack is the entry-tier evaluation procurement; production-scale workflows benefit substantially from stepping to 500 or 1000-rxn packs.

Application Profile

Single-target gene expression quantification — the workhorse application. Quantitative mRNA expression measurement from RNA template in one tube.

Viral RNA quantification (research-use) — pathogen-detection workflows where one-step format reduces contamination risk.

Low-copy RNA target detection — workflows where the one-step's preserved sensitivity matters.

Multi-sample single-target qPCR — gene expression workflows running the same target across multiple sample inputs.

Method-development RT-qPCR — protocol-development where one-step workflow simplification is the priority.

Multi-investigator shared facilities — facilities running standardized one-step RT-qPCR protocols.

Ordering & Configuration

Per-reaction workflow consumable. Each One-Step RT-qPCR reaction consumes proportional reagent.

Cycler-compatibility confirmation at order. High ROX is for Standard-block ABI 7000-series cyclers.

Storage. -20 °C, protect from light. RNase-free handling required throughout.

Pair with RNA template + sequence-specific primers. The kit includes the polymerase + RT + buffer + dNTPs + dye + RNase inhibitor + ROX; operator supplies RNA template and gene-specific primer pair.

Confirm at quote: cycler ROX requirement, workflow throughput, evaluation interest (PR2120-H-S sample at $14), one-step vs two-step decision (vs PR2110 + PR2000 separate cDNA + qPCR procurement), and lead time.

Warranty: Per Accuris standard reagent terms — confirm at quote.

Accuris PR2120 qMAX Green One-Step RT-qPCR Kit family — High ROX:

- PR2120-H-S — Sample, 20 reactions ($14)

- PR2120-H-100 (this product) — 100 reactions ($189, $1.89/rxn)

- PR2120-H-500 — 500 reactions ($366, $0.73/rxn)

- PR2120-H-1000 — 1000 reactions ($672, $0.67/rxn)

Pick the qMAX Green One-Step RT-qPCR for single-target gene expression / viral RNA quantification workflows; sister chemistry PR2121 (Probe One-Step) covers sequence-specific multiplex; sister ROX variants cover other cycler families.