The PR2000-N-50BLK is the 5,000-reaction bulk pack of the Accuris qMAX SYBR Green qPCR Master Mix in the No ROX formulation — a SYBR Green-based qPCR master mix for DNA template input (no reverse transcriptase included). Distinct from the PR2120 qMAX Green One-Step RT-qPCR Kit which combines RT + qPCR in one tube for RNA template input — this PR2000 master mix is the qPCR-only variant for workflows where the template is DNA (genomic DNA, plasmid, PCR product, cDNA from a separate RT reaction). Roughly half the price of the One-Step RT-qPCR kit at the same pack size ($2,196 vs $4,350 for PR2120-N-50BLK) reflects the simpler qPCR-only chemistry without the RT enzyme component.
Universe class flag: The universe price list classifies this SKU as capital, but a $2,196 reagent master-mix pack at 5,000 reactions is clearly consumable. Page authored as consumable; flagging for post-launch class correction.
Key Specifications
| SKU | PR2000-N-50BLK |
| Type | qPCR Master Mix — SYBR Green detection chemistry, DNA template input (no RT) |
| Reactions per pack | 5,000 reactions (bulk pack: 1 × 50 mL master mix) |
| Detection chemistry | SYBR Green I dye-based real-time qPCR |
| Template input | DNA (genomic DNA, plasmid, PCR product, separately-prepared cDNA) |
| Reverse transcriptase | NOT included (RT-qPCR workflows use the PR2120 One-Step RT-qPCR kit instead) |
| ROX formulation | No ROX — compatible with cyclers that don't require ROX normalization |
| Polymerase | Accuris Hot Start Taq with antibody-mediated hot-start activation |
| Cycler compatibility (No ROX) | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| Sister product (RT-qPCR variant) | PR2120-N-50BLK (One-Step RT-qPCR with RT enzyme included) |
| List price | $2,196 |
qPCR vs RT-qPCR — Why Two Separate Kits
The Accuris qMAX Green family ships in two formulations for different template inputs:
| Kit | Template input | Includes RT? | Price (5,000 rxn) |
| PR2000 qMAX SYBR Green qPCR (this kit's family) | DNA only | No | $2,196 |
| PR2120 qMAX Green One-Step RT-qPCR | RNA (one-step RT + qPCR in one tube) | Yes | $4,350 |
Pick the PR2000 qPCR-only kit when:
- The template is DNA (genomic, plasmid, PCR product, cDNA from a separate RT reaction)
- The lab does its own RT reaction separately and stores cDNA for batch qPCR
- Cost-per-reaction matters and the workflow doesn't need the One-Step convenience
Pick the PR2120 One-Step RT-qPCR kit when:
- The template is RNA and a one-step (RT + qPCR in one tube) workflow is preferred
- The workflow benefits from the reduced pipetting and contamination risk of the one-step format
ROX Variant Family — N / L / H
Same convention as the rest of the qMAX family:
| Suffix | ROX level | Compatible cyclers |
| N (this SKU) | No ROX | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| L | Low ROX | Applied Biosystems QuantStudio (newer), Stratagene Mx3000P/Mx3005P |
| H | High ROX | Applied Biosystems 7000/7300/7500/7900HT (older) |
Application Profile
DNA-template qPCR workflows — clinical-research and pharma R&D labs running qPCR on genomic DNA, plasmid DNA, or PCR product templates.
Two-step RT-qPCR workflows — labs that perform reverse transcription separately (using a dedicated RT kit) and store cDNA for batch qPCR analysis on the PR2000 qPCR mix.
Genotyping and variant-confirmation qPCR — workflows where DNA-template qPCR with melt-curve analysis confirms genotypes or variants.
Cost-effective qPCR primary screens — when the workflow uses DNA templates and per-reaction cost matters.
Bulk-pack institutional procurement — labs running >5,000 DNA-template qPCR reactions per year benefit from the bulk-pack per-reaction discount.
Ordering & Configuration
Storage & handling. Per kit instructions — typical qPCR master mix storage at −20 °C with limited freeze-thaw cycle tolerance.
Cycler compatibility verification. Critical at order: confirm the lab's real-time cycler platform requires the No ROX formulation. If the cycler requires Low ROX or High ROX, order the PR2000-L-50BLK or PR2000-H-50BLK variant instead.
Confirm at quote: real-time cycler platform and ROX requirement, template type (DNA — confirms PR2000 vs the PR2120 RT-qPCR sister kit), expected workflow throughput, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
qMAX SYBR Green qPCR Mix family — pack-size variants for No ROX:
- PR2000-N-50BLK (this product) — 5,000 reactions, bulk pack
- PR2000-N-25BLK — 2,500 reactions, smaller bulk pack
- PR2000-N-1000 — 1,000 reactions
- PR2000-N-500 — 500 reactions
- PR2000-N-100 — 100 reactions
- PR2000-N-S — sample size
For RNA template input requiring one-step RT-qPCR, see the PR2120 qMAX Green One-Step RT-qPCR Kit sister family. For probe-based detection (vs SYBR Green), see PR2001 qMAX Probe qPCR Mix.
