The PR2000-N-25BLK is the 2,500-reaction bulk pack of the Accuris qMAX SYBR Green qPCR Master Mix in the No ROX formulation — a SYBR Green-based qPCR master mix for DNA template input (no reverse transcriptase included), supplied as a single 25 mL bottle. Smaller bulk-pack sister to the PR2000-N-50BLK (5,000 reactions / 50 mL bottle) — same chemistry, half the reaction count and half the volume. Bulk packaging is positioned by Accuris for high-throughput users in pathogen detection, clinical assay, and cannabis-testing workflows where per-reaction cost economics matter.
Pricing note: The PR2000 PCR Bulk Reagents brochure prints a $1,030 list price for this pack; the 2026 universe price list specifies $1,220. Accuris has updated list pricing between the brochure issue date and the 2026 master price list — this page reflects the current 2026 list price. Confirm at quote.
Key Specifications
| SKU | PR2000-N-25BLK |
| Type | qPCR Master Mix — SYBR Green detection chemistry, DNA template input (no RT) |
| Reactions per pack | 2,500 reactions (bulk pack: 1 × 25 mL master mix bottle) |
| Concentration | 2× master mix |
| Detection chemistry | SYBR Green I dye-based real-time qPCR |
| Template input | DNA (genomic, plasmid, PCR product, separately-prepared cDNA) |
| Reverse transcriptase | NOT included (RT-qPCR workflows use the PR2120 One-Step RT-qPCR family instead) |
| ROX formulation | No ROX — compatible with cyclers that don't require ROX normalization |
| Polymerase | Accuris Hot Start Taq with antibody-mediated hot-start activation |
| Cycler compatibility (No ROX) | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| Larger bulk sister | PR2000-N-50BLK (5,000 reactions / 50 mL — same chemistry, larger pack) |
| Sister product (probe-based) | PR2001-N-25BLK (qMAX Probe — TaqMan / probe-based detection) |
| Sister product (RT-qPCR variant) | PR2120-N-25BLK (One-Step RT-qPCR with RT enzyme included) |
| List price | $1,220 |
| Returns | Non-returnable — perishable reagent per Accuris reagent-return policy |
Bulk-Pack Format — Why 25 mL vs 50 mL
Per the PR2000 PCR Bulk Reagents brochure, Accuris ships the qMAX Green family in two bulk-pack formats to match different workflow throughputs:
| Bulk pack | Format | Reactions | When to pick |
| PR2000-N-25BLK (this product) | 1 × 25 mL bottle | 2,500 reactions | High-throughput workflows where 2,500 reactions matches a quarterly or sub-annual reagent cycle without over-stocking |
| PR2000-N-50BLK | 1 × 50 mL bottle | 5,000 reactions | Highest-throughput workflows — pathogen-detection production labs, large clinical-research cohorts, multi-bench facilities consolidating procurement |
Both packs are the No ROX formulation. Pick the 25BLK pack when 2,500 reactions matches the workflow stocking cadence (quarterly turnover for moderate-throughput labs); pick the 50BLK pack when reagent throughput is fast enough that the 5,000-reaction pack turns over before stability becomes a concern.
qPCR vs RT-qPCR — Why Two Separate Kits
The Accuris qPCR family ships in two formulations for different template inputs:
| Kit | Template input | Includes RT? | Bulk pack 25BLK list (2026 universe) |
| PR2000 qMAX SYBR Green qPCR (this kit's family) | DNA only | No | $1,220 |
| PR2120 qMAX Green One-Step RT-qPCR | RNA (one-step RT + qPCR in one tube) | Yes | (See PR2120-N-25BLK page) |
Pick the PR2000 qPCR-only kit when:
- The template is DNA (genomic, plasmid, PCR product, cDNA from a separate RT reaction)
- The lab does its own RT reaction separately and stores cDNA for batch qPCR
- Cost-per-reaction matters and the workflow doesn't need One-Step convenience
Pick the PR2120 One-Step RT-qPCR kit when:
- Template is RNA and a one-step (RT + qPCR in one tube) workflow is preferred
- The workflow benefits from reduced pipetting and reduced contamination risk
ROX Variant Family — N / L / H
Same convention as the rest of the qMAX family — pick by cycler platform's ROX requirement:
| Suffix | ROX level | Compatible cyclers |
| N (this SKU) | No ROX | Bio-Rad CFX, Roche LightCycler, Eppendorf Mastercycler RealPlex |
| L | Low ROX | Applied Biosystems QuantStudio (newer), Stratagene Mx3000P / Mx3005P |
| H | High ROX | Applied Biosystems 7000 / 7300 / 7500 / 7900HT (older) |
The 25BLK and 50BLK bulk packs are currently shipped in the No ROX (N) formulation per the brochure ordering table; the L and H ROX bulk-pack variants may not be available — confirm at quote if the cycler requires Low or High ROX.
SYBR Green Detection Chemistry
SYBR Green I dye binds double-stranded DNA non-specifically; fluorescence signal increases as the qPCR amplicon accumulates. The qMAX SYBR Green chemistry:
- Hot-start Taq polymerase with antibody-mediated activation prevents nonspecific extension at low temperatures and reduces primer-dimer artifacts
- 2× master mix simplifies reaction setup (combine equal volumes master mix + sample / primers)
- Inert blue tracking dye (per the standard qMAX SYBR Green formulation) helps the operator visualize pipetting during reaction setup
For probe-based detection (TaqMan, molecular beacon, Scorpion), use the PR2001 qMAX Probe sister kit instead.
Application Profile
Pathogen-detection qPCR — DNA-template pathogen-detection assays (bacterial, fungal, viral DNA targets) where SYBR Green-based detection with melt-curve confirmation is the assay format.
Cannabis-testing qPCR — cannabis-genomics qPCR assays (sex determination, strain ID, pathogen testing on cannabis material).
Clinical-research DNA qPCR — clinical-research workflows on DNA templates (genomic SNP confirmation, copy-number qPCR, MRD-style DNA-based monitoring).
Two-step RT-qPCR workflows — labs that perform reverse transcription separately (using a dedicated RT kit) and store cDNA for batch qPCR analysis on the qMAX SYBR Green qPCR mix.
Genotyping and variant-confirmation qPCR — DNA-template qPCR with melt-curve analysis to confirm genotypes or sequence variants.
Cost-effective qPCR primary screens — large-scale primary screening workflows where the bulk-pack per-reaction cost makes the workflow economically viable.
Bulk-pack institutional procurement — labs running >2,500 DNA-template qPCR reactions per quarter benefit from the bulk-pack per-reaction discount.
Ordering & Configuration
Storage & handling. Per kit instructions — typical qPCR master mix storage at −20 °C with limited freeze-thaw cycle tolerance. The 25 mL single-bottle format requires the lab to plan working-aliquot workflow to limit freeze-thaw on the parent bottle.
Cycler compatibility verification. Critical at order: confirm the lab's real-time cycler platform requires the No ROX formulation. If the cycler requires Low ROX or High ROX, confirm whether bulk-pack L or H variants are currently available, or order in non-bulk pack sizes.
Working-aliquot practice. Single 25 mL bottle benefits from making working aliquots on first thaw to limit freeze-thaw cycles on the parent bottle.
Returns. Per the universe price-list category header: reagent products including qPCR mixes are non-returnable due to perishable nature.
Confirm at quote: real-time cycler platform and ROX requirement, template type (DNA — confirms PR2000 vs PR2120 RT-qPCR sister kit), expected workflow throughput, shipping conditions (cold-chain), and lead time.
Warranty: Per Accuris Life Science Reagents standard kit warranty terms — confirm at quote.
qMAX SYBR Green qPCR Mix family — bulk and pack-size variants for No ROX:
- PR2000-N-25BLK (this product) — 2,500 reactions, 1 × 25 mL bulk pack
- PR2000-N-50BLK — 5,000 reactions, 1 × 50 mL bulk pack (largest)
- PR2000-N-1000 / PR2000-N-500 / PR2000-N-100 — non-bulk pack sizes
- PR2000-N-S — sample size
For probe-based detection (TaqMan, Scorpion, molecular beacon), see the PR2001 qMAX Probe sister family. For RNA template input requiring one-step RT-qPCR, see the PR2120 qMAX Green One-Step RT-qPCR sister family.
